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Comparison of the Effects of Fucoidans on the Cell Viability of Tumor and Non-Tumor Cell Lines

1
Pharmaceutical Institute, Kiel University, Gutenbergstraße 76, 24118 Kiel, Germany
2
Department of Ophthalmology, University of Kiel, University Medical Center, Arnold-Heller-Str. 3, Haus 25, 24105 Kiel, Germany
3
GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Am Kiel-Kanal 44, 24106 Kiel, Germany
4
Faculty of Mathematics and Natural Sciences, Kiel University, Christian-Albrechts-Platz 4, 24118 Kiel, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work.
Mar. Drugs 2019, 17(8), 441; https://doi.org/10.3390/md17080441
Received: 26 June 2019 / Revised: 15 July 2019 / Accepted: 23 July 2019 / Published: 26 July 2019
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Abstract

Fucoidans extracted from brown algae exert manifold biological activities paving the way for the development of numerous applications including treatments outside tumor therapy such as age-related macular degeneration or tissue engineering. In this study, we investigated the antiproliferative effects of fucoidans extracted from six different algae (Fucus vesiculosus, F. serratus, F. distichus subsp. evanescens, Dictyosiphon foeniculaceus, Laminaria digitata, Saccharina latissima) as well as three reference compounds (Sigma fucoidan, heparin, enoxaparin) on tumor (HL-60, Raji, HeLa, OMM-1, A-375, HCT-116, Hep G2) and non-tumor (ARPE-19, HaCaT) cell lines. All fucoidans were extracted according to a standardized procedure and tested in a commercially available MTS assay. Cell viability was measured after 24 h incubation with test compounds (1–100 µg/mL). Apart from few exceptions, fucoidans and heparins did not impair cell viability. In contrast, fucoidans significantly increased cell viability of suspension cell lines, but not of adherent cells. Fucoidans slightly increased viability of tumor cells and had no impact on the viability of non-tumor cells. The cell viability of HeLa and ARPE-19 cells negatively correlated with protein content and total phenolic content (TPC) of fucoidans, respectively. In summary, none of the tested fucoidans turned out to be anti-proliferative, rendering them interesting for future studies and applications. View Full-Text
Keywords: Fucus vesiculosus; Fucus serratus; Fucus evanescens; Laminaria digitata; Saccharina latissima; Dictyosiphon foeniculaceus; heparin; cancer; cytotoxic; antiproliferative Fucus vesiculosus; Fucus serratus; Fucus evanescens; Laminaria digitata; Saccharina latissima; Dictyosiphon foeniculaceus; heparin; cancer; cytotoxic; antiproliferative
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Bittkau, K.S.; Dörschmann, P.; Blümel, M.; Tasdemir, D.; Roider, J.; Klettner, A.; Alban, S. Comparison of the Effects of Fucoidans on the Cell Viability of Tumor and Non-Tumor Cell Lines. Mar. Drugs 2019, 17, 441.

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