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Open AccessArticle

In Vitro Vascular-Protective Effects of a Tilapia By-Product Oligopeptide on Angiotensin II-Induced Hypertensive Endothelial Injury in HUVEC by Nrf2/NF-κB Pathways

1
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
2
School of Chemistry and Environmental Science, Guangdong Ocean University, Zhanjiang 524088, China
3
Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(7), 431; https://doi.org/10.3390/md17070431
Received: 3 June 2019 / Revised: 20 July 2019 / Accepted: 22 July 2019 / Published: 23 July 2019
Angiotensin II (Ang II) is closely involved in endothelial injury during the development of hypertension. In this study, the protective effects of the tilapia by-product oligopeptide Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) on oxidative stress and endothelial injury in Angiotensin II (Ang II)-stimulated human umbilical vein endothelial cells (HUVEC) were evaluated. LSGYGP dose-dependently suppressed the fluorescence intensities of nitric oxide (NO) and reactive oxygen species (ROS), inhibited the nuclear factor-kappa B (NF-κB) pathway, and reduced inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and endothelin-1 (ET-1) expression, as shown by western blot. In addition, it attenuated the expression of gamma-glutamyltransferase (GGT) and heme oxygenase 1 (HO-1), as well as increasing superoxide dismutase (SOD) and glutathione (GSH) expression through the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. Other experiments revealed that LSGYGP increased the apoptotic inhibition ratio between cleaved-caspase-3/procaspase-3, reduced expressions of pro-apoptotic ratio between Bcl-2/Bax, inhibited phosphorylation of mitogen-activated protein kinases (MAPK), and increased phosphorylation of the serine/threonine kinase (Akt) pathway. Furthermore, LSGYGP significantly decreased Ang II-induced DNA damage in a comet assay, and molecular docking results showed that the steady interaction between LSGYGP with NF-κB may be attributed to hydrogen bonds. These results suggest that this oligopeptide is effective in protecting against Ang II-induced HUVEC injury through the reduction of oxidative stress and alleviating endothelial damage. Thus, it has the potential for the therapeutic treatment of hypertension-associated diseases. View Full-Text
Keywords: tilapia; HUVEC; angiotensin II; NF-κB; Nrf2; endothelial dysfunction tilapia; HUVEC; angiotensin II; NF-κB; Nrf2; endothelial dysfunction
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MDPI and ACS Style

Chen, J.; Gong, F.; Chen, M.-F.; Li, C.; Hong, P.; Sun, S.; Zhou, C.; Qian, Z.-J. In Vitro Vascular-Protective Effects of a Tilapia By-Product Oligopeptide on Angiotensin II-Induced Hypertensive Endothelial Injury in HUVEC by Nrf2/NF-κB Pathways. Mar. Drugs 2019, 17, 431.

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