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Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources

1
MARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal
2
MARE—Marine and Environmental Sciences Centre, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal
3
BioCeramed, S.A., Rua José Gomes Ferreira nº 1 - Armazém D 2660-360 São Julião do Tojal, Portugal
4
CENIMAT/I3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia FCT, Universidade Nova de Lisboa, Campus da Caparica, 2829-516 Caparica, Portugal
5
Instituto Português do Mar e da Atmosfera (IPMA) Rua Alfredo Magalhães Ramalho, 6, 1449-006 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(4), 239; https://doi.org/10.3390/md17040239
Received: 27 January 2019 / Revised: 24 March 2019 / Accepted: 24 March 2019 / Published: 22 April 2019
(This article belongs to the Special Issue Marine Chitin 2019)
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Abstract

Chitin was extracted from Polybius henslowii, a swimming crab, captured in large quantities throughout the Portuguese coast by purse seine vessels as bycatch. After standard chitin extraction procedures, water-soluble chitosan products were obtained via two different methods: (1) N-acetylation with the addition of acetic anhydride and (2) a reaction with hydrogen peroxide. The chemical structure and molecular weight of chitosan derivatives, water-soluble chitosan (WSC) and chitooligosaccharides (COS), were confirmed by Fourier Transform Infrared Spectroscopy (FT-IR) and gel permeation chromatography (GPC). Antioxidant and metal chelation activities were evaluated, and the growth inhibition capacity was tested on four phytopatogens. The chitooligosaccharides from pereopods (pCOS) and shell body parts (sCOS) inhibited all fungal species tested, particularly Cryphonectria parasitica with 84.7% and 85.5%, respectively. Both radical scavenging and antifungal activities proved to be dose-dependent. Chitooligosaccharides with a low molecular weight (2.7, 7.4, and 10.4 Kg·mol−1) showed the highest activity among all properties tested. These results suggested that chitosan derivatives from P. henslowii raw material could potentially be used against phytopathogens or as ingredient in cosmetics and other products related to oxidative stress. View Full-Text
Keywords: Polybius henslowii; marine resources; chitosan; chitooligosaccharides; antifungal activity; antioxidant activity Polybius henslowii; marine resources; chitosan; chitooligosaccharides; antifungal activity; antioxidant activity
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MDPI and ACS Style

Avelelas, F.; Horta, A.; Pinto, L.F.; Cotrim Marques, S.; Marques Nunes, P.; Pedrosa, R.; Leandro, S.M. Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources. Mar. Drugs 2019, 17, 239.

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