Next Article in Journal
Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources
Previous Article in Journal
Dokdolipids A−C, Hydroxylated Rhamnolipids from the Marine-Derived Actinomycete Actinoalloteichus hymeniacidonis
Previous Article in Special Issue
Comparison of the Sulfonamide Inhibition Profiles of the α-Carbonic Anhydrase Isoforms (SpiCA1, SpiCA2 and SpiCA3) Encoded by the Genome of the Scleractinian Coral Stylophora pistillata
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle

Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines

1
Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Via U. Schiff 6, Sesto Fiorentino, 50019 Florence, Italy
2
Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy
3
Department of Chemistry, College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia
4
School of Chemistry, University of New South Wales, Dalton Building, Sydney, New South Wales 2052, Australia
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(4), 238; https://doi.org/10.3390/md17040238
Received: 25 March 2019 / Revised: 3 April 2019 / Accepted: 17 April 2019 / Published: 22 April 2019
(This article belongs to the Special Issue Carbonic Anhydrase in Marine Organism)
  |  
PDF [603 KB, uploaded 22 April 2019]
  |  

Abstract

The γ-carbonic anhydrases (CAs, EC 4.2.1.1) present in the Antarctic marine bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea, herein referred to as PhaCA and CpsCA, respectively, were investigated for their activation with a panel of 24 amino acids and amines. Both bacteria are considered Antarctic models for the investigation of photosynthetic and metabolic pathways in organisms adapted to live in cold seawater. PhaCA was much more sensitive to activation by these compounds compared to the genetically related enzyme CpsCA. The most effective PhaCA activators were d-Phe, l-/d-DOPA, l-Tyr and 2-pyridyl-methylamine, with the activation constant KA values of 0.72–3.27 µM. d-His, l-Trp, d-Tyr, histamine, dopamine, serotonin anddicarboxylic amino acids were also effective activators of PhaCA, with KA values of 6.48–9.85 µM. CpsCA was activated by d-Phe, d-DOPA, l-Trp, l-/d-Tyr, 4-amino-l-Phe, histamine, 2-pyridyl-methylamine and l-/d-Glu with KA values of 11.2–24.4 µM. The most effective CpsCA activator was l-DOPA (KA of 4.79 µM). Given that modulators of CAs from Antarctic bacteria have not been identified and investigated in detail for their metabolic roles to date, this research sheds some light on these poorly understood processes. View Full-Text
Keywords: Antarctic bacteria; carbonic anhydrase; metalloenzymes; amine; amino acid; activator; Pseudoalteromonas haloplanktis; Colwellia psychrerythraea Antarctic bacteria; carbonic anhydrase; metalloenzymes; amine; amino acid; activator; Pseudoalteromonas haloplanktis; Colwellia psychrerythraea
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Angeli, A.; Del Prete, S.; Osman, S.M.; AlOthman, Z.; Donald, W.A.; Capasso, C.; Supuran, C.T. Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines. Mar. Drugs 2019, 17, 238.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top