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Mar. Drugs 2019, 17(3), 188; https://doi.org/10.3390/md17030188

Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid Ommastrephes bartrami

1
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China
2
Marine School, Ningbo University, Ningbo 315000, China
3
Zhejiang Mariculture Research Institute, Zhoushan 316000, China
*
Author to whom correspondence should be addressed.
Received: 6 February 2019 / Revised: 5 March 2019 / Accepted: 15 March 2019 / Published: 22 March 2019
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Abstract

To explore bioactive polysaccharides from the byproducts of squid processing, a heteropolysaccharide, named SV2-1, was isolated from the viscera of squid Ommastrephes bartrami by autolysis, anion-exchange and gel-permeation chromatography and measured for its neuroprotective activity. It was a homogeneous polysaccharide with a molecular weight of 2.3 kDa by HPSEC analysis. SV2-1 contained glucuronic acid, galactosamine and fucose in the ratio of 1.0:1.1:1.2. Its structural characteristics were elucidated by methylation analysis, gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR). The backbone of SV2-1 was composed of alternant →4)-α-l-Fucp-(1→ and →3)-β-d-GlcUA-(1→ Most of →4)-α-l-Fucp-(1→ (90%) was substituted by single α-d-GlcNAc as the branches. SV2-1 can protect against the death of PC12 induced by 6-OHDA, and effectively improves cell viability and reduces extracellular LDH release in PC12 cells after injury. Moreover, SV2-1 significantly increases SOD activity but decreases MDA levels. View Full-Text
Keywords: polysaccharide; squid autolysate; structure; neuroprotective polysaccharide; squid autolysate; structure; neuroprotective
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Ye, P.; Li, P.; Yang, W.; Zhao, Y.; Zhao, Y.; Sun, K.; Wang, B.; Chen, Y. Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid Ommastrephes bartrami. Mar. Drugs 2019, 17, 188.

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