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2-Alkyl-4-hydroxyquinolines from a Marine-Derived Streptomyces sp. Inhibit Hyphal Growth Induction in Candida albicans

1
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
2
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(2), 133; https://doi.org/10.3390/md17020133
Received: 2 February 2019 / Revised: 18 February 2019 / Accepted: 20 February 2019 / Published: 22 February 2019
(This article belongs to the Special Issue Bioactive Compounds from Marine Microbes - II)
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Abstract

Four 2-alkyl-4-hydroxyquinoline derivatives (14) were isolated from a semisolid rice culture of the marine-derived actinomycete Streptomyces sp. MBTG13. The structures of these compounds were elucidated by a combination of spectroscopic methods, and their data were in good agreement with previous reports. Compounds 1 and 2 exhibited weak to moderate antibacterial activity against pathogenic bacteria. Unexpectedly, we found that compound 1 acted as a potent inhibitor of hyphal growth induction in the dimorphic fungus Candida albicans, with an IC50 value of 11.4 μg/mL. Growth experiments showed that this compound did not inhibit yeast cell growth, but inhibited hyphal growth induction. Semi-quantitative reverse transcription (RT)-PCR analysis of hyphal-inducing signaling pathway components indicated that compound 1 inhibited the expression of mRNAs related to the cAMP-Efg1 pathway. The expression of HWP1 and ALS3 mRNAs (hypha-specific genes positively regulated by Efg1, an important regulator of cell wall dynamics) was significantly inhibited by the addition of compound 1. These results indicate that compound 1 acts on the Efg1-mediated cAMP pathway and regulates hyphal growth in Candida albicans. View Full-Text
Keywords: 2-alkyl-4-hydroxyquinolines; marine actinomycete; Streptomyces sp.; Candida albicans; morphogenesis; cAMP-Efg1 pathway 2-alkyl-4-hydroxyquinolines; marine actinomycete; Streptomyces sp.; Candida albicans; morphogenesis; cAMP-Efg1 pathway
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Kim, H.; Hwang, J.-Y.; Chung, B.; Cho, E.; Bae, S.; Shin, J.; Oh, K.-B. 2-Alkyl-4-hydroxyquinolines from a Marine-Derived Streptomyces sp. Inhibit Hyphal Growth Induction in Candida albicans. Mar. Drugs 2019, 17, 133.

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