Next Article in Journal
Synthesis and Antiproliferative Activity of Marine Bromotyrosine Purpurealidin I and Its Derivatives
Next Article in Special Issue
Analgesic Activity of Acid-Sensing Ion Channel 3 (ASIС3) Inhibitors: Sea Anemones Peptides Ugr9-1 and APETx2 versus Low Molecular Weight Compounds
Previous Article in Journal
A Soft Coral-Derived Compound, 11-Dehydrosinulariolide, Induces G2/M Cell Cycle Arrest and Apoptosis in Small Cell Lung Cancer
Previous Article in Special Issue
Two Novel Multi-Functional Peptides from Meat and Visceral Mass of Marine Snail Neptunea arthritica cumingii and Their Activities In Vitro and In Vivo
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2018, 16(12), 480; https://doi.org/10.3390/md16120480

Modulation of Human Complement System by Antimicrobial Peptide Arenicin-1 from Arenicola marina

1
Institute of Experimental Medicine, Acad. Pavlov Str. 12, Saint Petersburg 197376, Russia
2
Research Institute of Highly Pure Biopreparations, Pudozhskaya Str., 7, Saint Petersburg 197110, Russia
3
Department of Biochemistry, Saint-Petersburg State University, Universitetskaya Embankment, 7/9, Saint-Petersburg 199034, Russia
4
M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow 117997, Russia
*
Author to whom correspondence should be addressed.
Received: 17 October 2018 / Revised: 13 November 2018 / Accepted: 27 November 2018 / Published: 1 December 2018
Full-Text   |   PDF [1858 KB, uploaded 1 December 2018]   |  

Abstract

Antimicrobial peptides from marine invertebrates are known not only to act like cytotoxic agents, but they also can display some additional activities in mammalian organisms. In particular, these peptides can modulate the complement system as was described for tachyplesin, a peptide from the horseshoe crab. In this work, we investigated the influence on complement activation of the antimicrobial peptide arenicin-1 from the marine polychaete Arenicola marina. To study effects of arenicin on complement activation in human blood serum, we used hemolytic assays of two types, with antibody sensitized sheep erythrocytes and rabbit erythrocytes. Complement activation was also assessed, by the level of C3a production that was measured by ELISA. We found that the effect of arenicin depends on its concentration. At relatively low concentrations the peptide stimulates complement activation and lysis of target erythrocytes, whereas at higher concentrations arenicin acts as a complement inhibitor. A hypothetical mechanism of peptide action is proposed, suggesting its interaction with two complement proteins, C1q and C3. The results lead to the possibility of the development of new approaches for therapy of diseases connected with complement dysregulation, using peptide regulators derived from natural antimicrobial peptides of invertebrates. View Full-Text
Keywords: Arenicola marina; antimicrobial peptides; arenicin; complement; C3a Arenicola marina; antimicrobial peptides; arenicin; complement; C3a
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Umnyakova, E.S.; Gorbunov, N.P.; Zhakhov, A.V.; Krenev, I.A.; Ovchinnikova, T.V.; Kokryakov, V.N.; Berlov, M.N. Modulation of Human Complement System by Antimicrobial Peptide Arenicin-1 from Arenicola marina. Mar. Drugs 2018, 16, 480.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top