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Mar. Drugs 2018, 16(12), 473; https://doi.org/10.3390/md16120473

Two Novel Multi-Functional Peptides from Meat and Visceral Mass of Marine Snail Neptunea arthritica cumingii and Their Activities In Vitro and In Vivo

1
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China
2
Shandong Provncial Engineering Laboratory for Biological Testing Technology, Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Jinan 250103, China
3
Research Center for Marine Drugs, State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacy, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 31 October 2018 / Revised: 20 November 2018 / Accepted: 22 November 2018 / Published: 27 November 2018
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Abstract

Neptunea arthritica cumingii (Nac) is a marine snail with high nutritional and commercial value; however, little is known about its active peptides. In this study, two multi-functional peptides, YSQLENEFDR (Tyr-Ser-Gln-Leu-Glu-Asn-Glu-Phe-Asp-Arg) and YIAEDAER (Tyr-Ile-Ala-Glu-Asp-Ala-Glu-Arg), were isolated and purified from meat and visceral mass extracts of Nac using a multi-bioassay-guided method and were characterized by using liquid chromatography-tandem mass spectrometry. Both peptides showed high antioxidant, angiotensin-converting enzyme (ACE)-inhibitory, and anti-diabetic activities, with half-maximal effective concentrations values less than 1 mM. Antioxidant and ACE-inhibitory activities were significantly higher for YSQLENEFDR than for YIAEDAER. In a zebrafish model, the two peptides exhibited strong scavenging ability for reactive oxygen species and effectively protected skin cells against oxidative damage without toxicity. Molecular docking simulation further predicted the interactions of the two peptides and ACE. Stability analysis study indicated that the two synthetic peptides maintained their activities under thermal stress and simulated gastrointestinal digestion conditions. The low molecular weight, high proportion of hydrophobic and negatively-charged amino acids, and specific C-terminal and N-terminal amino acids may contribute to the observed bio-activities of these two peptides with potential application for the prevention of chronic noncommunicable diseases. View Full-Text
Keywords: Neptunea arthritica cumingii; multi-functional peptides; antioxidant activity; ACE-inhibitory activity; anti-diabetic activity Neptunea arthritica cumingii; multi-functional peptides; antioxidant activity; ACE-inhibitory activity; anti-diabetic activity
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Zhang, S.-S.; Han, L.-W.; Shi, Y.-P.; Li, X.-B.; Zhang, X.-M.; Hou, H.-R.; Lin, H.-W.; Liu, K.-C. Two Novel Multi-Functional Peptides from Meat and Visceral Mass of Marine Snail Neptunea arthritica cumingii and Their Activities In Vitro and In Vivo. Mar. Drugs 2018, 16, 473.

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