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Open AccessArticle

Anticoagulant Properties of a Green Algal Rhamnan-type Sulfated Polysaccharide and Its Low-molecular-weight Fragments Prepared by Mild Acid Degradation

by Xue Liu 1,2,3, Peng Du 1, Xiao Liu 1, Sujian Cao 1, Ling Qin 1, Meijia He 1, Xiaoxi He 1,2 and Wenjun Mao 1,2,*
1
Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
2
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
3
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2018, 16(11), 445; https://doi.org/10.3390/md16110445
Received: 29 September 2018 / Revised: 25 October 2018 / Accepted: 6 November 2018 / Published: 12 November 2018
(This article belongs to the Special Issue Marine Polysaccharides in Pharmaceutical Applications)
The active sulfated polysaccharide from seaweed possesses important pharmaceutical and biomedical potential. In the study, Monostroma sulfated polysaccharide (MSP) was obtained from Monostroma angicava, and the low-molecular-weight fragments of MSP (MSP-Fs: MSP-F1–MSP-F6) were prepared by controlled acid degradation. The molecular weights of MSP and MSP-F1–MSP-F6 were 335 kDa, 240 kDa, 90 kDa, 40 kDa, 24 kDa, 12 kDa, and 6.8 kDa, respectively. The polysaccharides were sulfated rhamnans that consisted of →3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→ units with partial sulfation at C-2 of →3)-α-l-Rhap-(1→ and C-3 of →2)-α-l-Rhap-(1→. Anticoagulant properties in vitro of MSP and MSP-F1–MSP-F6 were evaluated by studying the activated partial thromboplastin time, thrombin time, and prothrombin time. Anticoagulant activities in vivo of MSP and MSP-F4 were further evaluated; their fibrin(ogen)olytic activities in vivo and thrombolytic properties in vitro were also assessed by D-dimer, fibrin degradation products, plasminogen activator inhibitior-1, and clot lytic rate assays. The results showed that MSP and MSP-F1–MSP-F4 with molecular weights of 24–240 kDa had strong anticoagulant activities. A decrease in the molecular weight of MSP-Fs was accompanied by a decrease in the anticoagulant activity, and higher anticoagulant activity requires a molecular weight of over 12 kDa. MSP and MSP-F4 possessed strong anticoagulant activities in vivo, as well as high fibrin(ogen)olytic and thrombolytic activities. MSP and MSP-F4 have potential as drug or helpful food supplements for human health. View Full-Text
Keywords: sulfated polysaccharide; depolymerization; low-molecular-weight fractions; anticoagulant activity; fibrin(ogen)olytic activity; thrombolytic activity sulfated polysaccharide; depolymerization; low-molecular-weight fractions; anticoagulant activity; fibrin(ogen)olytic activity; thrombolytic activity
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MDPI and ACS Style

Liu, X.; Du, P.; Liu, X.; Cao, S.; Qin, L.; He, M.; He, X.; Mao, W. Anticoagulant Properties of a Green Algal Rhamnan-type Sulfated Polysaccharide and Its Low-molecular-weight Fragments Prepared by Mild Acid Degradation. Mar. Drugs 2018, 16, 445.

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