Next Article in Journal / Special Issue
Positional Distribution of Fatty Acids in Triacylglycerols and Phospholipids from Fillets of Atlantic Salmon (Salmo Salar) Fed Vegetable and Fish Oil Blends
Previous Article in Journal
Structure and Effects of Cyanobacterial Lipopolysaccharides
Previous Article in Special Issue
Hyperoxia Elevates Adrenic Acid Peroxidation in Marine Fish and Is Associated with Reproductive Pheromone Mediators
Article Menu

Export Article

Mar. Drugs 2015, 13(7), 4231-4254;

Advances in Microalgae-Derived Phytosterols for Functional Food and Pharmaceutical Applications
1,2, 1,2 and 1,2,*
Flinders Centre for Marine Bioproducts Development, Flinders University, Adelaide, SA 5042, Australia
Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide, SA 5042, Australia
Author to whom correspondence should be addressed.
Academic Editor: Gilles Barnathan
Received: 8 June 2015 / Accepted: 29 June 2015 / Published: 9 July 2015


Microalgae contain a variety of bioactive lipids with potential applications in aquaculture feed, biofuel, food and pharmaceutical industries. While microalgae-derived polyunsaturated fatty acid (PUFA) and their roles in promoting human health have been extensively studied, other lipid types from this resource, such as phytosterols, have been poorly explored. Phytosterols have been used as additives in many food products such as spread, dairy products and salad dressing. This review focuses on the recent advances in microalgae-derived phytosterols with functional bioactivities and their potential applications in functional food and pharmaceutical industries. It highlights the importance of microalgae-derived lipids other than PUFA for the development of an advanced microalgae industry.
microalgae; lipids; phytosterols; functional food; pharmaceuticals

1. Phytosterols: Chemistry, Origin and Applications

1.1. Chemistry of Phytosterols

There has been no standardisation of phytosterol nomenclature. The most commonly adopted phytosterol nomenclature is in the International Union of Pure and Applied Chemistry and International Union of Biochemistry recommendations 1989 [1] (Figure 1). As shown, phytosterols are characterised by a tetracyclic cyclopenta (α) phenanthrene structure (ring A, B, C and D) and an aliphatic side chain (R) at C17 of ring D. They are amphiphilic due to the polar hydroxyl group (OH) at C3 of ring A and have a non-polar structure for the rest. In most cases, phytosterols have a double bond between C5 and C6 and methyl groups at C10 and C13. From domain D, the length, position of double bond, absence or presence of a methyl or ethyl group, saturation and stereochemistry of the C24 alkyl side chain are critical to intermolecular contacts and function of phytosterols [2]. Phytosterols may also be represented using CxΔy where x indicates the total carbon number and y shows the location of double bonds [3]. Most of the microalgal phytosterols are in the free form with a relatively small number of conjugated forms. Conjugates are present as phytosterols with covalently bounded molecules particularly fatty acids and sugars at the OH group at C3 [4].
Figure 1. Nomenclature of phytosterols. Numbering follows the International Union of Pure and Applied Chemistry and International Union of Biochemistry 1989 recommendations with modifications [1].
Figure 1. Nomenclature of phytosterols. Numbering follows the International Union of Pure and Applied Chemistry and International Union of Biochemistry 1989 recommendations with modifications [1].
Marinedrugs 13 04231 g001

1.2. Origin and Applications of Phytosterols

There are more than 100 different types of phytosterols, which are under the triterpene family of nature products [5]. Cholesterol is the predominant sterol in animals whereas it is barely found in plants. Instead, plants contain several types of phytosterols, which are structurally similar and functionally analogous to cholesterols [6]. Phytosterols are present in all eukaryotic organisms, through either de novo synthesis or taken up from the environment [7]. They are important structural components of the cellular membrane and have important functions in regulating membrane fluidity and permeability. They also exist as hormones or hormonal precursors and are involved in signal transductions in the organisms [6].
Unlike cholesterol, humans cannot endogenously synthesise phytosterols and have to gain them from diet [8]. In the western diet, the average daily intake of phytosterols (mainly from vegetable oils, cereals, and fruits) is around 250 mg [9]. This amount of intake is estimated to be doubled for vegetarians [10]. Since the mid-1990s, phytosterol products have been commercialised as nutraceuticals or pharmaceuticals with the ability of lowering the blood cholesterol level, such as Cytellin marketed by Eli Lilly [11]. The main ingredient of Cytellin is sitosterol, which was used as either a supplement or as a drug for lowering cholesterol [12]. However, the market of phytosterols has not been revived until 1990s when Miettinen, Vanhanen [13] solved the issue of poor solubility and bioavailability of free phytosterols and achieved the consistency of the cholesterol-lowering effects with minimum amount of intake (2–3 g/day). Apart from its therapeutic values to treat hypercholesterolemia, phytosterols are also applied in other pharmaceutical areas as precursors of some bioactive molecules. An example is ergosterol as a precursor of vitamin D2 and an ingredient for producing cortisone and hormone flavone [14]. According to the Phytosterols Market Analysis by GVR [15], the global phytosterol demand was 49,299.6 tons (estimated revenue of USD 292.8 million) in 2013 and is expected to reach 80,535.9 tons (estimated revenue of 989.8 million) in 2020. Because of a forecasted increase in phytosterol market demand, alternative sources with high phytosterol content will generate great research and industry interest.

1.3. Health Promoting Effects of Phytosterol and Its Regulations

Phytosterols have received great attention because of its capacity of reducing the concentration of blood cholesterol and preventing the onset of cardiovascular disorders. In 2000, FDA issued an interim final rule authorising health claims for reduced risk of coronary heart disease (CHD) for phytosterol esters containing foods (65 FR54686) [16]. In 2010, FDA authorised the fortification of foods using nonesterified or free phytosterols. There are three phytosterols subject to the FDA health claim: β-sitosterol, campesterol and stigmasterol [17]. The Board of Food Standards Australia New Zealand (Proposal P1025) also gave notice of safe use of phytosterols and their esters in foods including breakfast cereals, cereal bars, milk and yoghurt. The European Atherosclerosis Society Consensus Panel approved the utilisation of phytosterol-enriched foods among patients with high cholesterol levels [18]. This favourable regulatory scenario is again going to further propel the global market growth of phytosterol and its products.

2. Microalgae as a Potential Source of Phytosterols

2.1. Types of Phytosterols from Microalgae for Human Consumption

To date, higher plants have been the main industrial sources of phytosterols [11], which are naturally present in vegetable oils, legumes, nuts, seeds, whole grains and dried fruits [4,19]. They are also found in algae: Chlorophyceae, Rhodophyceae and Phaeophyceae [6]. A standard reference regarding phytosterol content in different food sources can be found in United States Department of Agriculture’s National Nutrient Database. Phytosterol contents varied from 8.09 to 15.57 g per kg (equivalent to 0.809%–1.557% of oil weight) in corn oil, 19.7 g per kg (1.97%) in wheat germ oil and 32.25g per kg (3.225%) in rice bran oil [7]. In contrast, phytosterol content ranged from 7 to 34 g per kg (0.7%–3.4%) in four different microalgae oil extracts (Isochrysis galbana, Nannochloropis gaditana, Nannochloropsis sp. and Phaeodactylum tricornutum) depending on the solvent system being chosen [20]. More recently, Pavlova lutheri, Tetrasellimis sp. M8 and Nannochloropsis sp. BR2 were identified as the top three highest phytosterol producers (0.4%–2.6% dry weight) after screening hundreds of Australian isolates [21]. Research has reported that 5.1% dry weight of phytosterol could be achieved in P. lutheri by adjusting the nutrient, salinity and cultivation duration. Given that these phytosterol contents on the basis of microalgae dry biomass weight are equal or higher than all the plant oils extracted, it presents clear advantages to use microalgae directly as phytosterol supplements in various applications. The other two reasons that microalgae might be more advantageous to other common sources are their fast-growing characteristics and rich nutrient content [22]. The annual oil yield of some oil rich microalgal species varies from 19,000 to 57,000 L oil per acre, which is 60–200 times higher than the best-performing vegetable oils [23]. Some of them are also rich in other nutrients including proteins, carbohydrates, vitamins (vitamin A, B1, B2, B6, B12 and K, folate, niacin), minerals (calcium, phosphorous, iron, iodine, magnesium, zinc, selenium, copper, potassium, manganese and sodium) and various antioxidants (carotenoids, xanthophylls and chlorophyll) [24,25]. Many researches have reported the side effects of short and long term consumption of phytosterols as interfering with the absorption of β-carotenoid and vitamin E [4]. Therefore, it is suggested that dietary intake of these nutrients has to be increased at the same time to offset this absorption interference caused by phytosterol [26]. Microalgae may offer a complete and easy option due to its high vitamins, antioxidants and phytosterol contents and solve the issue of multi-supplementations for dietary needs.
In contrast with higher plants, there is a larger diversity of sterol distributions in microalgae [27] as listed in Table 1. Among microalgae, Glaucocystophyte are characterised by the presence of sitosterol and campesterol [28], Cyanobacteria by 24-ethylcholesterol [3], Cryptophytes by epibrassicasterol [29], Haptophytes by unusual dihydroxysterol from the genus Pavlova [30], Pelagophyceae by unusual 24-propylidenecholesterol mainly as the 24E-isomer [31], marine diatoms by 4-desmethyl-23,24-dimethyl steroid [32], Prasinophyceae by 24-methylenecholesterol and campesterol [33], Chlorarachniophyceae by crinosterol and stigmasterol [34], and most dinoflagellates by 4α-methyl sterols (especially dinosterol) [35] with the exception of Kareniaceae and Polarella glacialis [36].
Microalgae-derived phytosterols can be divided into four groups, 4-desmethyl-Δ5-sterols, 4-desmetyl-Δ7-sterols, 4-methyl sterols and dihydroxylated sterols [3]. The predominant phytosterol obtained from microalgae has Δ5 double bond and has no methyl groups at C4. Volkman (2003) summarised the occurrence of major sterols in different families of microalgae. Most sterols have 27 to 29 carbon atoms. Some species are exceptions. For example, the dinoflagellate Prorocentrum contains trace amount of 23 carbon sterol, the diatom Chaetoceros contains 26 carbon sterol, and Chrysophyte Sarcinochrysis and Nematochrysopsis contain 30 carbon sterol [37,38]. The composition of phytosterols varies depending on the strain and can be affected by factors such as light intensity, temperature and growth stage [39,40]. Some species such as dinoflagellates may contain a mixture of ten or even more sterol types [3]. With high phytosterol content in biomass and structure diversity between species, microalgae are promising sources of novel phytosterols with potential novel bioactivities.
Table 1. Example of phytosterols identified from different microalgae species. Common names are used where applicable.
Table 1. Example of phytosterols identified from different microalgae species. Common names are used where applicable.
SpeciesIdentified PhytosterolsReferences
Attheya ussurensis sp. nov.24-Ethylcholest-5-en-3β-ol[27]
BigelowiellaCrinosterol, Stigmasterol[34]
Chattonella antiqueIsofucosterol[41]
Chattonella marinaIsofucosterol[41]
Chattonella subsalsaIsofucosterol[41]
Chlorella vulgarisErgosterol, 7-Dehydroporiferasterol, Ergosterol peroxide, 7-Oxocholesterol[42]
Chrysoderma sp.Stigmasterol, Sitosterol, Fucosterol[38]
ChrysomerisStigmasterol, Sitosterol, Fucosterol[38]
ChrysowaernellaStigmasterol, Sitosterol, Fucosterol[38]
Crypthecodinium cohnii4α-Methyl sterols, Dinosterols, Dehydrodinosterol
Cyanophora paradoxaSitosterol, Campesterol and
Diacronema vlkianum24-Ethylcholesta-5,22E-dien-3β-ol
Dunaliella salinaErgosterol, 7-Dehydroporiferasterol,
7-Dehydroporiferasterol peroxide, Ergosterol peroxide
Dunaliella tertiolectaErgosterol, 7-Dehydroporiferasterol[44,46]
Fragilaria pinnata23,24-Dimethylcholesta-5,22E-dien-3β-ol[47]
GiraudyopsisStigmasterol, Sitosterol, Fucosterol[38]
Glaucocystis nostochinearumSitosterol, Campesterol,
GymnochloraCrinosterol, Stigmasterol[34]
Isochrysis galbana24-Oxocholesterol acetate, Ergost-5-en-3β-ol,
Karenia brevis27-Nor-(24R)-4α-methyl-5α-ergosta-8(14),22-dien-3β-ol
its 27-Nor derivative
Karenia mikimotoi27-Nor-(24R)-4α-methyl-5α-ergosta-8(14),22-dien-3β-ol
Brevesterol, Gymnodinosterol
Karenia papilionacea23-Methyl-27-norergosta-8(14),22-dien-3β-ol[49]
Karenia umbella(24R)-4α-Methyl-5α-ergosta-8(14),22-dien-3β-ol
Karlodinium veneficum(24R)-4α-Methyl-5α-ergosta-8(14),22-dien-3β-ol
LotharellaCrinosterol and Stigmasterol[34]
Micromonas aff.pusilla24-Methycholesta-5,24(28)-dien-3β-ol
28-Isofucosterol and saringosterol
Micromonas pusilla24-Methycholesta-5,24(28)-dien-3β-ol
Navicula incertaStigmasterol, 5β-Hydroxysitostanol[52,53]
Nematochrysopsis sp.(24E)-24-n-propylidenecholesterol[38]
Nitzschia closteriumCholesta-5,24-dien-3β-ol
Nostoc commune var. sphaeroides KützingCampesterol, Sitosterol, Clionasterol[54,55]
Olisthodiscus luteusBrassicasterol, Stigmasterol, Fucosterol[41]
Phaeodactylum tricornutum(24S)-24-Methylcholesta-5,22E-dien-3β-ol[39]
Polarella glacialis27-Nor-24-Methylcholest-5,22E-dien-3β-ol[35]
Porphyridium cruentumStigmasterol, β-Sitosterol[56]
Pycnococcus provasolii24-Methycholesta-5,24(28)-dien-3β-ol
Pyramimonas cf. cordataStigmasterol[27]
Pyramimonas cordata24-Methycholesta-5,24(28)-dien-3β-ol
Rhizosolenia setigeraCholesta-5,24-dien-3β-ol[47]
Sarcinochrysis sp.(24E)-24-n-propylidenecholesterol[38]
Schizochytrium aggregatumCampesterol, 24-Methylene cholesterol, Ergosterol,
24-Methyl-colest-7-en-3β-ol, Stigmasterol and others
Schizochytrium sp.Lathosterol, Ergosterol, Stigmasterol,
24-Ethylcholesta-5,7,22-trienol, Stigmasta-7,24-(241)-dien-3β-ol,
Stephanodiscus meyerii24-Methycholesta-5,24(28)-dien-3β-ol[27]
Takayama helix27-Nor-(24R)-4α-methyl-5α-ergosta-8(14),22-dien-3β-ol
Takayama tasmanica27-Nor-(24R)-4α-methyl-5α-ergosta-8(14),22-dien-3β-ol
Tetraselmis chui24-Methycholesta-5,24(28)-dien-3β-ol
Tetraselmis suecica24-Methylcholest-5-en-3β-ol
Thalassi-onema nitzschioides23-Methylcholesta-5,22E-dien-3β-ol

2.2. Biosynthesis of Phytosterols in Microalgae

The occurrence of sterols varies among plants, microorganisms, prokaryotes, yeasts and algae [59]. Similar to other organisms, microalgal phytosterols are also the end products of isoprenoid biosynthesis, from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) to squalene. Some microalgae retain two distinct and compartmentalised pathways for isoprenoid synthesis, mevalonic acid (MVA) pathway in the cytosol and the methyl-d-erythritol 4-phosphate (MEP) pathway in the plastid [7]. These microalgae arise from secondary endosymbiosis including Euglenophyta, Chlorarachniophyta, Heterokontophyta, Bacillariophyta and Haptophyta [59]. Some using both pathways may also arise from primary endosymbiosis such as Glaucophyta [60,61]. Exceptions are Prasinophyta and Chlorophyta, arising from primary endosymbiosis. They have completely lost the MVA pathway and exclusively produce sterols from MEP pathway. The exceptions within these two families are microalgae Galdieria sulphuraria [62] and Cyanidium caldarium [63], both of which use MVA pathway. A comprehensive review on the distribution of pathways of different microalgae families can be found by Lohr, Schwender [60]. The side chain of microalgal phytosterol contains an alkyl substitution at C24, which is added by sterol methyltransferase (SMT) in a step other than MEP or MVA pathways. This pathway is not necessary for the biosynthesis of some 27 carbon sterols [6].

2.3. Bio-Functionalities of Microalgal Phytosterols and Their Mechanisms of Action

Phytosterols have been reported to have many beneficial health effects in humans, including immunomodulatory [46], anti-inflammatory [42,46], antihypercholesterolemic [54,58], antioxidant [64], anticancer [65,66] and antidiabetic [67]. Table 2 summarises the microalgal phytosterols undergoing functional tests. As shown, even though microalgae-derived phytosterols are diverse, limited studies have addressed their health-promoting activities.

2.3.1. Cholesterol-Lowering Activity

Many studies have reported the cholesterol-lowering activity of consuming phytosterols and their esters, with 10%–15% reduction of low density lipoprotein serum cholesterol (LDL-C: major risk factors for CHD) shown among individuals with hypercholesterolemia [4]. The reduction was even more outstanding among patients who have been put on anti-hypercholesterolemic drugs such as statins [68] and fibrates [69]. The cholesterol-lowering activity was also observed for microalgae-derived phytosterols, which functioned by decreasing the dietary cholesterol absorption and endogenously-produced cholesterols from the gastrointestinal tract [58].
Schizochytrium sterol extract down-regulated the expression of intestinal gene ACAT2 [58], which is responsible for cholesterol absorption in the intestine [70]. The cholesterol-lowering ability of this extract is also related to the down-regulation of hepatic 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, which is an enzyme involved in the synthesis of cholesterol. Meanwhile, Schizochytrium sterols stimulated the LDL-C receptor that facilitates the removal of plasma cholesterol from the circulation. Research showed that hamsters being fed on 0.06 and 0.3 g of Schizochytrium sterol extract per kg diet demonstrated a reduction of cholesterol level by 19.5% and 34%, respectively. The bioactivity of Schizochytrium sterol extract was as effective as the positive control group supplied with β-sitosterol, which is a phytosterol already added to food products, such as margarine and vegetable oils as healthy supplements. The mechanisms of action among different phytosterols are not the same. The lipid extract of blue-green alga, Nostoc commune var. sphaeroides Kützing (N. commune) has an inhibitory effect in cholesterol synthesis on human hepatoma cell lines by reducing the mRNA expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and LDL receptor [54]. The lipid of interest was not identified in this research; however, a previous study has reported that this species was characterized by the presence of campesterol, β-sitosterol and clionasterol [71]. Cholesterol-lowering activity of β-sitosterol is achieved by competing with cholesterol for transporter NPC1L1 as well as down-regulating its mRNA expression in the gastrointestinal tract [58]. β-Sitosterol are also found in some microalgae species (Table 3), however with their activity yet to be determined. As shown, some microalgae species have demonstrated a great potential in lowering plasma cholesterol and should be further explored.

2.3.2. Anti-Inflammatory Activity

Ergosterol isolated from edible mushrooms has the ability to suppress LPS-induced inflammatory responses of RAW264.7 macrophages in vitro through the inhibition of highly proinflammatory cytokine (TNF-α) production and COX-2 expression [72]. Ergosterol was found in Chlorella vulgaris [42] and Dunalliella tertiolecta, which demonstrated a similar mechanism of action via the reduction of LPS-induced response [46]. Apart from ergosterol, ergosterol peroxide, 7-dehydroporiferasterol peroxide and 7-oxocholesterol from Chlorella vulgaris also showed effective anti-inflammatory activities on 12-O-tetradecanoylphorbol-13-acetate (TPA: a potent tumour promoter)-induced mice with 0.2–0.7 mg/ear as 50% inhibitory dose [42]. The microalga Dunaliella tertiolecta has also been recently identified with ergosterol, 7-dehydroporiferasterol and ergosterol peroxide, and considered as future commercial source of phytosterol [44,45]. Some research reported a synergistic mechanism of some microalgal phytosterols regarding the enhancement of the bioactivity of another phytosterol. For example, the mixture of 7-dehydroporiferasterol with ergosterol (both from Dunalliella tertiolecta) further suppressed the proliferation of concanavalin A (ConA)-stimulated ovine peripheral blood mononuclear cells (PBMCs) compared with ergosterol alone at the same concentration [46]. Microalgal phytosterols and their secondary metabolites are promising potential anti-inflammatory agents and the synergistic effect should always take into consideration when optimising the functionality.

2.3.3. Anticancer Activity

Several studies have reported that phytosterols may have bioactivities against tumours [73]. For example, ergosterol showed cytostatic effect on human colorectal adenocarcinoma cells [72]. Ergosterol peroxide showed inhibitory effect on the growth of MCF-7 human mammary adenocarinoma and Walker 256 carcinosarcoma cells in vitro [74]. 2 μmol ergosterol peroxide from Chlorella vulgaris remarkably inhibited (77% reduction) the tumour progression by TPA and 7,12-dimethylbenz[a]anthracene (DMBA: immunosuppressor and tumour initiator)-initiated mice [42]. It was suggested that these bioactive sterols were functioned by inhibiting the accumulation of ornithine decarboxylase (ODC), which is a polyamine biosynthetic enzyme induced by TPA treatment. Stigmasterol isolated from Navicula incerta showed a significant toxicity on hepatocarcinoma (HepG2) cells in a dose-dependent manner and are effective to induce apoptosis via the up-regulation of pro-apoptotic gene Bax and p53 and down-regulation of the anti-apoptotic gene Bcl-2 [52,53]. Fucosterols and oxygenated fucosterol isolated from brown alga Sargassum carpophyllum exhibited cytotoxicity against different cancer cell lines [65,75]. The same phytosterols have also been identified in microalgae Chrysoderma sp. and Olisthodiscus luteus (Table 3); however, with functionality undetermined. Collectively, microalgae are promising resource for chemopreventive agents in cancer therapy but further studies are required to identify the equality between the phytosterols of interest and those derived from microalgae species.

2.3.4. Antioxidant

Evidence of antioxidant activity from microalgae-derived phytosterols was in scarcity. However, phytosterols derived from other resources have been reported showing positive effects. For instance, stigmasterol derived from the bark of Butea monosperma showed highest pro-oxidative dose of 5.2 mg per kg of food intake per day by reducing the tissue lipid peroxidation (major cause of cellular damage) and increasing the activities of catalase, superoxide dismutase (SOD) and glutathione, which are endogenous antioxidants [76]. In addition, carbon tetrachloride (CCl4)-intoxicated rats, undergoing treatment with 30 mg (fucosterol derived from marine algae Pelvetia siliquosa) per kg of food intake per day for seven consecutive days, showed significant decrease of serum transaminase activities and increase of free radical scavenging enzymes such as SOD, catalase and glutathione peroxidise by 33.89%, 21.56% and 39.24%, respectively [64]. Even though fucosterols have been found in several microalgae species such as Chrysoderma sp., Chrysomeris, Chrysowaernella and Giraudyopsis [38], no research could be found specifically analysing the activity of microalgae-derived fucosterols. Lipid extracts of some microalgae species have been identified with antioxidant activity, such as Schizochytrium aggregatum [57], however, the compounds of interest remain to be determined. This identification is urgently required, because of the health concern and risks caused by synthetic antioxidants in the market such as butylated hydroxytoluen (BHT) and propyl gallate (PG) used in food and pharmaceutical industries [77]. Microalgal phytosterols as natural products are more preferable alternatives for antioxidants for human consumption.

2.3.5. Other Activities

Other activities relating to microalgae-derived phytosterols include antibacterial and antidiabetic activities. Tuberculosis is the second most common cause of human death, and it is contagious and airborne [78]. Prakash, Sasikala [48] found that the extract of microalgae Isochrysis galbana (with 24-oxocholesterol acetate, ergost-5-en-3β-ol and cholest-5-en-24-1,3-(acetyloxy)-,3β-ol) at 50 μg per mL inhibited multidrug resistant Mycobacterium tuberculosis compared to the tuberculosis drug amikacin at 700 μg per mL, pyrazinamide at 200 μg per mL and rifambicin at 40 μg per mL. Furthermore, 0.5 μg per mL saringosterol isolated from brown algae Sargassum ringgoldianum, could also inhibit the growth of M. tuberculosis H37Rv, which has been found to be as efficient as tuberculosis drug rifampicin in the same assay [79]. Saringosterol has also been found in microalgae Micromonas aff. pusilla [51] but with undefined bioactivity. Diabetes is a chronic disease characterised by high blood glucose level and acute complications such as hypoglycaemia. Fucosterols isolated from the seaweed Pelvetia siliquosa have been identified with anti-diabetic activity in streptozotocin-induced diabetic rats [67]. Several microalgae species have also been reported producing fucosterols such as Chrysoderma sp. and Olisthodiscus luteus [38,41]; however, the functionality of these fucosterol are remained to be tested. Due to the high annual yield of microalgae lipids, microalgae-derived phytosterols as natural products [44] have a much greater potential to the drug industry; which may not only solve the issue of the upcoming surge of the global demand but also offer more preferable alternatives due to side effects associated with synthetic drugs.
Table 2. Bioactivities of phytosterols derived from microalgae. Abbreviation: DPPH: 2, 2-diphenyl-1-picrylhydrazyl; HMGR: 3-hydroxy-3-methylglutaryl-CoA reductase; SREBP-1: sterol regulatory element binding protein -1.
Table 2. Bioactivities of phytosterols derived from microalgae. Abbreviation: DPPH: 2, 2-diphenyl-1-picrylhydrazyl; HMGR: 3-hydroxy-3-methylglutaryl-CoA reductase; SREBP-1: sterol regulatory element binding protein -1.
Microalgae SpeciesMajor PhytosterolsBiological ActivityFunctionReferences
Chlorella vulgarisErgosterol,
Ergosterol peroxide,
7-Dehydroporiferasterol peroxide,
Anti-inflammatory50% inhibitory dose was 0.2–0.7 mg/ear[42]
Chlorella vulgarisErgosterol peroxideAnti-cancer2 μmol led to 77% reduction in tumour progression[42]
Dunaliella tertiolectaErgosterol,
Immunomodulatory Anti-inflammatory0.4 mg/mL mixture for the highest production of IL-10, 0.8mg/mL for ergosterol alone[46]
Dunaliella tertiolectaErgosterol,
NeuromodulatoryNeuromodulatory action was found in selective brain areas of rats[80]
Isochrysis galbana24-Oxocholesterol acetate,
3β-ol and others
AntituberculosisMinimum inhibitory concentration of 50–60 μg/mL against M. tuberculosis[48]
Navicula incertaStigmasterol,
Anti-cancer40%, 43% and 54% toxicity at 5, 10 and 20 μM, respectively[52,53]
Nostoc commune var. sphaeroides KützingLipid extractCholesterol-lowering activityReduced HMGR activity by 90% and reduced SREBP-1 mature protein by 30%[54]
Schizochytrium aggregatumCampesterol,
24-Methylene cholesterol,
Ergosterol, Stigmasterol and other lipids
AntioxidantIC50 in DPPH radical scavenging study was 5.76 mg/mL.
Digested microalgae oil had an α-tocopherol equivalent antioxidant capacity of 42.071 μg/mg
At 10 mg/mL, reducing power was 0.874
Schizochytrium sp.Lathosterol, Ergosterol, Stigmasterol,
Stigmasta-7,24-(241)-dien-3β-ol and others
Cholesterol-lowering activity0.06–0.3 g/kg diet decreased blood cholesterol by 19.5%–34%[58]
Table 3. Functional phytosterols observed in microalgae and the original resources for the function identification. Information on nomenclatures gathered from [4] with adaptations.
Table 3. Functional phytosterols observed in microalgae and the original resources for the function identification. Information on nomenclatures gathered from [4] with adaptations.
Chemical StructureNomenclaturesSpecies of OriginBioactivitySame Sterol(s) Observed in Microalgae
Campesterol Marinedrugs 13 04231 i001Campesterin
Flower Chrysanthemum coronarium L. [66]
Red algae Porphyra dentata [81]
Shorea singkawang [82]
Tetraselmis [33]
Porphyridium cruentum [83]
Schizochytrium aggregatum [57]
7-Dehydroporiferasterol Marinedrugs 13 04231 i002(22E,24R)-Ethylcholesta-5,7,22-trien-3β-ol
Rarely found in other organisms-Chlorella vulgaris [84]
Chlamydomonas reinhardtii [85]
Dictyonella incisa [86]
Ergosterol Marinedrugs 13 04231 i003(22E)-Ergosta-5,7,22-trien-3β-ol
Mushroom Sarcodon aspratus [72]
Mushroom Inonotus obliquus [87]
Ganoderma lucidum [88]
Agaricus bisporus [89]
Chlorella pyranoidosa [90]
Dunaliella tertiolecta [80]
Schizochytrium aggregatum [57]
Fucosterol Marinedrugs 13 04231 i004(24(28)E)-Stigmasta-5,24(28)-dien-3β-ol
Macroalgae Pelvetia siliquosa [64,67]
Brown alga Turbinaria conoides [65]
Macroalgae Himanthalia elongate, Undaria pinnatifida, Phorphyra sp., Chondus crispus, Cystoseira sp. and Ulva sp. [91]
Chrysoderma sp.
Giraudyopsis [38]
Olisthodiscus luteus [41]
Saringosterol Marinedrugs 13 04231 i00524(S)-Saringosterol
Sargasso sterol
Brown algae Sargassum ringgoldianum [79]
Sargassum thunbergii [92]
Lessonia nigrescens [93]
Seaweed Sargassum fusiforme [94]
Micromonas aff.pusilla [51]
β-Sitosterol Marinedrugs 13 04231 i006Sitosterol
Peanuts [95]
Coral subergorgia reticulate [96]
Plant Verbena officinalis [97]
Leaves of Mentha cordifolia Opiz [98]
Analgesic activity
Bigelowiella natans
Gymnochlora stellata
Lotharella amoeboformis [34]
Porphyridium cruentum [56]
Stigmasterol Marinedrugs 13 04231 i0075,22E) (24α=24S)
Butea monosperma [76]
Parkia speciosa seeds [99]
Thyroid-inhibitory Antioxidant
Porphyridium cruentum [56]
Δ5-Avenasterol Marinedrugs 13 04231 i008(5-Avenasterol) (Δ5,24Z)
Brown algae Fucus vesiculosus
Green algae Ulva lactuca [100]
Wheat germ oil [101]
Tomato seed oil [102]
Sargassum thunbergii [92]
Rape bee pollen [103]
Marine sponge Petrosia weinbergi [104]
Antioxidant Lipase-inhibitory
Precursor of antiviral orthoesterol
Chlorophyceae [55]
Chattonella marina [41]
Brassicasterol Marinedrugs 13 04231 i00924-Methyl cholest-5,22-dien-3β-ol
Rapeseed oil [105]Cholesterol-loweringIsochrysis galbana and Chaetoceros calcitrans [106]
Rhodomonas salina [107]

2.4. Advanced Green Extraction and Purification Technology of Phytosterols

The industry-scale technology for phytosterol recovery utilises sterol containing materials such as tall oils or vegetable oils and is processed by hydrolysation of the steryl esters into free sterols [11]. This process is rather complex, energy intensive and involves organic solvents and toxic chemicals, such as chloroform, methanol, hexane and sodium hydroxide [108,109,110,111]. The same extraction process was also found when extracting phytosterols from D. tertiolecta, D. salina [44] and Pyramimonas cf. cordata [27]. Even though substantial yield of phytosterols might be achieved, the application of toxic chemicals may hamper its application in food and pharmaceutical industry.
Methods for green extraction technology of phytosterols, include supercritical carbon dioxide extraction (SC-CO2) [112,113,114,115,116,117]. Carbon dioxide has been used as the first choice solvent in more than 90% of the supercritical fluid extraction of bioactive compounds from natural resources [118], due to the benefits of safe, inexpensive, recyclable and being non-hazardous to health and environment [119]. Optimization of extraction parameters such as pressure, temperature, flow rate of CO2 was required when perform SC-CO2. This processing technology applies to various sources and is considered to be an effective and environmentally friendly technique for the separation of solvent-free phytosterols [120]. SC-CO2 has also been applied for general microalgal lipid production [121,122]; however, it has been rarely integrated with high performance liquid chromatography (HPLC) or gas chromatography-mass spectrometry (GC-MS) to specifically analyze the phytosterol component. SC-CO2 is a clean and food safety-guaranteed extraction method. Future research should investigate more into applying this technology to phytosterol production from microalgae biomass.

3. Future Prospects of Microalgae-Derived Phytosterols

One of the limitations in the development of microalgae-derived phytosterols is their low sterol content [3,44]. Compared with other commercial plant sources, some microalgae species show an equivalent phytosterol content and this figure may surpass that found in some conventional sources when choosing the best performing microalgae strain with optimised cultivation conditions. In recent years, phytosterols from microalgae are starting to attract more attention due to the diversity of phytosterols in these species. The utilisation of microalgae for phytosterols production offers an opportunity for finding novel phytosterols with potential benefits to human health or a mixture of molecules able to synergistically enhance the bioactivity of a single phytosterol [46]. 7-Dehydroporiferasterol acting as a good example of phytosterol derived from microalgae with outstanding anti-inflammatory activities but has rarely been observed in other organisms. Over the last decade, researchers have started to analyse the bioactive phytosterols isolated from macroalgae [65,75,123,124,125] but studies on microalgal sterols have lagged far away behind. Most microalgal sterol research was conducted on the analysis and characterisation of sterol components within different species (Table 1) but with very limited amount of studies focusing on the bioactivities and functionalities of those sterols (Table 2). For the studies with identified bioactivities, further endeavours should be aimed at identifying the sterol species responsible for the activity.
The screening of microalgal sterols for bioactivity could be directed by the chemical structure of the sterol of interest. A close dependency between the skeletal structure of sterols and their bioactivity has been reported as evidenced by the remarkable anticancer activity within groups of Δ5,7-sterol, 5α,8α-epidioxy-Δ6-sterols and 7-oxo-Δ5-sterol [42]. It was suggested that the double bonds at C5 and C22 in phytosterols are responsible for the apoptosis induction effect [52]. In the same vein, Hernandez-Ledesma, Blanca [6] and Nes [126] reported that rings A and D are of particular importance to sterol’s function and the stereochemistry of the C24 alkyl group is the key to intermolecular interactions. This importance on sterol structure is also proved by the addition of an oxo group at C7 of cholesterol, a Δ5-sterol, which showed poor inflammatory activity on its own. However, after the addition of oxo group, the 7-oxo-Δ5-sterol considerably increased the anti-inflammatory effect on TPA-induced inflammatory mice [42]. The same theory also applied to Δ-5-avenasterol which showed antioxidant activity due to the presence of ethyliden group in 24, 28 position of the R chain [102]. Microalgal species rich in docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA) may also offer a clue for the screening process. Previous studies on DHA and DPA derived from Schizochytrium sp. were identified with cholesterol-lowering activity through the down-regulation of HMG-CoA reductase [127]. The later study observed that the sterols derived from this species also contributed to the activity [58].
Given microalgal phytosterols are diverse, it is essential to differentiate the potential of the different types of sterols in microalgae, including their mechanism of actions, synergic effects with other compounds and the effects of long-term treatment. To emphasise that, the same sterols isolated from different microalgae species might be in a mixture of epimers [93]. The function of the epimers should always be questioned compared to the pure ones. This is because sargosterol (mixture of 24S and 24R epimers) isolated from Lessonia nigrescens were eight times more active against M. tuberculosis H37Rv than 24S isomer alone [93]. Even though microalgae-derived phytosterols have rarely been reported with toxicity, some microalgae like Dinophyceae class may produce toxins especially during harmful algal blooms [128]. Bioactive phytosterol extracts should also undergo toxicity assays (organisms-based or cell line-based) and chemical analysis (such as LC-MS) to verify their applicability in food and pharmaceutical industries [129]. Elimination of toxins, such as okadaic acid, dinophysistoxins and brevetoxins, is compulsory before further processing. In addition, phytosterol-fortified foods are rich in free phytosterols and their fatty acid esters, which are susceptible to oxidation, future research should also investigate the stability of microalgal phytosterol when applied to food fortification. The compounds resulting from phytosterol oxidation could exert toxic effects and initiate the major chronic diseases [130]. This could be done by the analysis of the production of phytosterol oxidation products (POPs). To improve the oxidative stability, phytosterols could be incorporated to a matrix with natural antioxidant compounds such as milk based fruit beverages [131].

4. Conclusions

Phytosterols have grown in popularity due to their health-promoting activities over the past few decades. New sources are urgently needed to meet the growing demand of phytosterols for functional food and pharmaceutical industries. Microalgae as one of the best alternatives could offer different types of phytosterols and other high-valued compounds at a much higher efficiency than terrestrial plants. However, the research on microalgal phytosterols is mainly focusing on the area of analysing and identifying sterol constituents with most of their bioactivities unknown. Thus, research in the future should focus more on the functional activity of microalgae-derived phytosterols and their applications in food and pharmaceutical industries.


Thank you to Shirley Sorokin for edits on the manuscript. Thanks also go to financial support from Centre for Marine Bioproducts Development, Flinders University; and Flinders University for their APA scholarship to Xuan Luo.

Author Contribution

Xuan Luo coordinated the data collection for writing this manuscript, structured this manuscript, wrote the major part of the manuscript and did the proofreading. Peng Su helped to collect the data to develop the manuscript and helped to write the manuscript. Wei Zhang helped to plan and structured the manuscript, and also refined the graphs and tables and proof-read the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.


  1. Moss, G.P. Nomenclature of steroids (Recommendations 1989). Pure Appl. Chem. 1989, 61, 1783–1822. [Google Scholar] [CrossRef]
  2. Trautwein, E.A.; Demonty, I. Phytosterols: natural compounds with established and emerging health benefits. Ol. Corps Gras Lipides 2007, 14, 259–266. [Google Scholar] [CrossRef]
  3. Volkman, J. Sterols in microorganisms. Appl. Microbiol. Biotechnol. 2003, 60, 495–506. [Google Scholar] [CrossRef] [PubMed]
  4. Moreau, R.A.; Whitaker, B.D.; Hicks, K.B. Phytosterols, phytostanols, and their conjugates in foods: Structural diversity, quantitative analysis, and health-promoting uses. Prog. Lipid Res. 2002, 41, 457–500. [Google Scholar] [CrossRef]
  5. Goad, J.L. Methods in Plant Biochemistry; Academic Press: London, UK, 1991; pp. 369–434. [Google Scholar]
  6. Hernandez-Ledesma, B.; Herrero, M. Bioactive Compounds from Marine Foods: Plant and Animal Sources, 1st ed.; John Wiley & Sons Ltd: Chichester, UK, 2014; pp. 173–187. [Google Scholar]
  7. Piironen, V.; Lindsay, D.G.; Miettinen, T.A.; Toivo, J.; Lampi, A.M. Plant sterols: Biosynthesis, biological function and their importance to human nutrition. J.Sci. Food Agric. 2000, 80, 939–966. [Google Scholar] [CrossRef]
  8. Geçgel, M.T.B.B.Ü.; Demirci, A. Phytosterols as functional food ingredients. J. Tekirdag Agric. Fac. 2006, 3, 153–159. [Google Scholar]
  9. Hicks, K.B.; Moreau, R.A. Phytosterols and phytostanols: Functional food cholesterol busters. Food Technol. 2001, 55, 63–67. [Google Scholar]
  10. Cerqueira, M.T.; Fry, M.M.; Connor, W.E. The food and nutrient intakes of the Tarahumara Indians of Mexico. Am. J. Clin. Nutr. 1979, 32, 905–915. [Google Scholar] [PubMed]
  11. Fernandes, P.; Cabral, J.M.S. Phytosterols: Applications and recovery methods. Bioresour. Technol. 2007, 98, 2335–2350. [Google Scholar] [CrossRef] [PubMed]
  12. Pollak, O.J. Reduction of blood cholesterol in man. Circulation 1953, 7, 702–706. [Google Scholar] [CrossRef] [PubMed]
  13. Miettinen, T.; Vanhanen, H.; Wester, I. Use of a Stanol Fatty Acid Ester for Reducing Serum Cholesterol Level. U.S. Patent 5,502,045, 26 March 1996. [Google Scholar]
  14. Tan, T.; Zhang, M.; Gao, H. Ergosterol production by fed-batch fermentation of Saccharomyces cerevisiae. Enzym. Microb. Technol. 2003, 33, 366–370. [Google Scholar] [CrossRef]
  15. Global Phytosterols Market By Application (Pharmaceuticals, Cosmetics, Food Ingredients), By Product (Beta-sitosterol, Campesterol, Stigmasterol) is Expected to Reach USD 989.8 Million by 2020: Grand View Research, Inc. Available online: (accessed on 6 July 2015).
  16. FDA. 65 FR 54686—Food Labeling: Health Claims; Plant Sterols/Stanol Esters and Coronary Heart Disease; Federal FR Doc No: 00-22892; Office of the Federal Register, National Archives and Records Administration: Washington, DC, USA, 2000.
  17. Srigley, C.T.; Haile, E.A. Quantification of plant sterols/stanols in foods and dietary supplements containing added phytosterols. J. Food Compos. Anal. 2015, 40, 163–176. [Google Scholar] [CrossRef]
  18. Gylling, H.; Plat, J.; Turley, S.; Ginsberg, H.N.; Ellegård, L.; Jessup, W.; Jones, P.J.; Lütjohann, D.; Maerz, W.; Masana, L. Plant sterols and plant stanols in the management of dyslipidaemia and prevention of cardiovascular disease. Atherosclerosis 2014, 232, 346–360. [Google Scholar] [CrossRef] [PubMed]
  19. Piironen, V.; Toivo, J.; Lampi, A.M. Natural sources of dietary plant sterols. J. Food Compos. Anal. 2000, 13, 619–624. [Google Scholar] [CrossRef]
  20. Ryckebosch, E.; Bruneel, C.; Termote-Verhalle, R.; Muylaert, K.; Foubert, I. Influence of extraction solvent system on extractability of lipid components from different microalgae species. Algal Res. 2014, 3, 36–43. [Google Scholar] [CrossRef]
  21. Ahmed, F.; Zhou, W.; Schenk, P.M. Pavlova lutheri is a high-level producer of phytosterols. Algal Res. 2015, 10, 210–217. [Google Scholar] [CrossRef]
  22. Chisti, Y. Biodiesel from microalgae. Biotechnol. Adv. 2007, 25, 294–306. [Google Scholar] [CrossRef] [PubMed]
  23. Demirbas, A.M.; Fatih, D.M. Importance of algae oil as a source of biodiesel. Energy Convers. Manag. 2011, 52, 163–170. [Google Scholar] [CrossRef]
  24. Brown, M.R. Nutritional Value and Use of Microalgae in Aquaculture. In Proceedings of the Avances en Nutrición Acuícola VI. Memorias del VI Simposium Internacional de Nutrición Acuícola, Cancún, Quintana Roo, México, 3–6 September, 2002; pp. 281–292.
  25. Tang, G.; Suter, P.M. Vitamin A, nutrition, and health values of algae: Spirulina, Chlorella, and Dunaliella. J. Pharm. Nutr. Sci. 2011, 1, 111–118. [Google Scholar] [CrossRef][Green Version]
  26. QuÍlez, J.; GarcÍa-Lorda, P.; Salas-Salvadó, J. Potential uses and benefits of phytosterols in diet: Present situation and future directions. Clin. Nutr. 2003, 22, 343–351. [Google Scholar] [CrossRef]
  27. Ponomarenko, L.P.; Stonik, I.V.; Aizdaicher, N.A.; Orlova, T.Y.; Popovskaya, G.I.; Pomazkina, G.V.; Stonik, V.A. Sterols of marine microalgae Pyramimonas cf. cordata (Prasinophyta), Attheya ussurensis sp. nov. (Bacillariophyta) and a spring diatom bloom from Lake Baikal. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2004, 138, 65–70. [Google Scholar] [CrossRef] [PubMed]
  28. Leblond, J.D.; Timofte, H.I.; Roche, S.A.; Porter, N.M. Sterols of glaucocystophytes. Phycol. Res. 2011, 59, 129–134. [Google Scholar] [CrossRef]
  29. Goad, L.J.; Holz, G.G., Jr.; Beach, D.H. Identification of (24S)-24-methylcholesta-5,22-dien-3β-ol as the major sterol of a marine cryptophyte and a marine prymnesiophyte. Phytochemistry 1983, 22, 475–476. [Google Scholar] [CrossRef]
  30. Volkman, J.K.; Farmen, C.L.; Barrett, S.M.; Sikes, E.L. Unusual dihydroxysterols as chemotaxonoic markers for microalgae from the order Pavlovales (Haptophyceae). J. Phycol. 1997, 33, 1016–1023. [Google Scholar] [CrossRef]
  31. Giner, J.L.; Zhao, H.; Boyer, G.L.; Satchwell, M.F.; Andersen, R.A. Sterol chemotaxonomy of marine Pelagophyte algae. Chem. Biodivers. 2009, 6, 1111–1130. [Google Scholar] [CrossRef] [PubMed]
  32. Rampen, S.W.; Schouten, S.; Hopmans, E.C.; Abbas, B.; Noordeloos, A.A.M.; van Bleijswijk, J.D.L.; Geenevasen, J.A.J.; Sinninghe, D.J.S. Diatoms as a source for 4-desmethyl-23,24-dimethyl steroids in sediments and petroleum. Geochim. Cosmochim. Acta 2009, 73, 377–387. [Google Scholar] [CrossRef]
  33. Patterson, G.W.; Tsitsa-Tzardis, E.; Wikfors, G.H.; Gladu, P.K.; Chitwood, D.J.; Harrison, D. Sterols of Tetraselmis (Prasinophyceae). Comp. Biochem. Physiol. B Comp. Biochem. 1993, 105, 253–256. [Google Scholar] [CrossRef]
  34. Leblond, J.D.; Dahmen, J.L.; Seipelt, R.L.; Elrod-Erickson, M.J.; Kincaid, R.; Howard, J.C.; Evens, T.J.; Chapman, P.J. Lipid composition of chlorarachniophytes (Chlorarachniophyceae) from the Genera Bigelowiella, Gymnochlora, and Lotharella. J. Phycol. 2005, 41, 311–321. [Google Scholar] [CrossRef]
  35. Thomson, P.G.; Wright, S.W.; Bolch, C.J.S.; Nichols, P.D.; Skerratt, J.H.; McMinn, A. Antarctic distribution, pigment and lipid composition, and molecular identification of the brine dinoflagellate Polarella glacialis (Dinophyceae). J. Phycol. 2004, 40, 867–873. [Google Scholar] [CrossRef]
  36. Kumari, P.; Kumar, M.; Reddy, C.R.K.; Jha, B. Algal Lipids Fat. Acids Sterols; Woodhead Publishing Limited: Cambridge, UK, 2013. [Google Scholar]
  37. Volkman, J.; Rijpstra, W.I.C.; de Leeuw, J.W.; Mansour, M.P.; Jackson, A.E.; Blackburn, S.I. Sterols of four dinoflagellates from the genus Prorocentrum. Phytochemistry 1999, 52, 659–668. [Google Scholar] [CrossRef]
  38. Billard, G.; Dauguet, J.C.; Maume, D.; Bert, M. Sterols and chemotaxonomy of marine Chrysophyceae. Bot. Mar. 1990, 33, 225–228. [Google Scholar] [CrossRef]
  39. Fábregas, J.; Arán, J.; Morales, E.D.; Lamela, T.; Otero, A. Modification of sterol concentration in marine microalgae. Phytochemistry 1997, 46, 1189–1191. [Google Scholar] [CrossRef]
  40. Piretti, M.V.; Pagliuca, G.; Boni, L.; Pistocchi, R.; Diamante, M.; Gazzotti, T. Investigating of 4-methyl sterols from cultured dinoflagellate algal strains. J. Phycol. 1997, 33, 61–67. [Google Scholar] [CrossRef]
  41. Marshall, J.A.; Nichols, P.; Hallegraeff, G. Chemotaxonomic survey of sterols and fatty acids in six marine raphidophyte algae. J. Appl. Phycol. 2002, 14, 255–265. [Google Scholar] [CrossRef]
  42. Yasukawa, K.; Akihisa, T.; Kanno, H.; Kaminaga, T.; Izumida, M.; Sakoh, T.; Tamura, T.; Takido, M. Inhibitory effects of sterols isolated from Chlorella vulgaris on 12-O-tetradecanoylphorbol-13-acetate-Induced inflammation and tumor promotion in mouse skin. Biol. Pharm. Bull. 1996, 19, 573–576. [Google Scholar] [CrossRef] [PubMed]
  43. Mendes, A.; Reis, A.; Vasconcelos, R.; Guerra, P.; da Silva, T.L. Crypthecodinium cohnii with emphasis on DHA production: A review. J. Appl. Phycol. 2009, 21, 199–214. [Google Scholar] [CrossRef]
  44. Francavilla, M.; Trotta, P.; Luque, R. Phytosterols from Dunaliella tertiolecta and Dunaliella salina: A potentially novel industrial application. Bioresour. Technol. 2010, 101, 4144–4150. [Google Scholar] [CrossRef]
  45. Sheffer, M.; Fried, A.; Gottlieb, H.E.; Tietz, A.; Avron, M. Lipid composition of the plasma-membrane of the halotolerant alga, Dunaliella salina. Biochim. Biophys. Acta 1986, 857, 165–172. [Google Scholar] [CrossRef]
  46. Caroprese, M.; Albenzio, M.; Ciliberti, M.G.; Francavilla, M.; Sevi, A. A mixture of phytosterols from Dunaliella tertiolecta affects proliferation of peripheral blood mononuclear cells and cytokine production in sheep. Vet. Immunol. Immunopathol. 2012, 150, 27–35. [Google Scholar] [CrossRef] [PubMed]
  47. Barrett, S.M.; Volkman, J.K.; Dunstan, G.A.; LeRoi, J.M. Sterols of 14 species of marine diatoms (bacillariophyta). J. Phycol. 1995, 31, 360–369. [Google Scholar] [CrossRef]
  48. Prakash, S.; Sasikala, S.; Aldous, V.H.J. Isolation and identification of MDR-Mycobacterium tuberculosis and screening of partially characterised antimycobacterial compounds from chosen marine micro algae. Asian Pac. J. Trop. Med. 2010, 3, 655–661. [Google Scholar] [CrossRef]
  49. Mooney, B.D.; Nichols, P.D.; de Salas, M.F.; Hallegraeff, G.M. Lipid, fatty acid, and sterol composition of eight species of kareniaceae (Dinophyta): Chemotaxonomy and putative lipid phycotoxin. J. Phycol. 2007, 43, 101–111. [Google Scholar] [CrossRef]
  50. Leblond, J.D.; Evans, T.J.; Chapman, P.J. The biochemistry of dinoflagellate lipids, with particular reference to the fatty acid and sterol composition of a Karenia brevis bloom. Phycologia 2003, 42, 324–331. [Google Scholar] [CrossRef]
  51. Volkman, J.K.; Barrett, S.M.; Dunstan, G.A.; Jeffrey, S.W. Sterol biomarkers for microalgae from the green algal class Prasinophyceae. Org. Geochem. 1994, 21, 1211–1218. [Google Scholar] [CrossRef]
  52. Kim, Y.S.; Li, X.F.; Kang, K.H.; Ryu, B.; Kim, S.K. Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells. BMB Rep. 2014, 47, 433. [Google Scholar] [CrossRef] [PubMed]
  53. Kim, S.K.; KANG, K.H.; Kim, Y.S. Pharmaceutical Composition for Preventing or Treating Liver Cancer Comprising Stigmasterol and 5 beta-hydroxysitostanol Isolated from Navicula incerta. U.S. Patent 2014/0057884 A1, 27 February 2014. [Google Scholar]
  54. Rasmussen, H.E.; Blobaum, K.R.; Park, Y.K.; Ehlers, S.J.; Lu, F.; Lee, J.Y. Lipid extract of Nostoc commune var. sphaeroides Kützing, a blue-green alga, inhibits the activation of sterol regulatory element binding proteins in HepG2 Cells. J. Nutr. 2008, 138, 476–481. [Google Scholar]
  55. Paoletti, C.; Pushparaj, B.; Florenzano, G.; Capella, P.; Lercker, G. Unsaponifiable matter of green and blue-green algal lipids as a factor of biochemical differentiation of their biomasses: II. Terpenic alcohol and sterol fractions. Lipids 1976, 11, 266–271. [Google Scholar] [CrossRef]
  56. Durmaz, Y.; Monteiro, M.; Koru, E.; Bandarra, N. Concentration of sterols of Porphyridium cruentum biomass at stationary phase. Pak. J. Biol. Sci. 2007, 10, 1144–1146. [Google Scholar] [CrossRef] [PubMed]
  57. Lv, J.; Yang, X.; Ma, H.; Hu, X.; Wei, Y.; Zhou, W.; Li, L. The oxidative stability of microalgae oil (Schizochytrium aggregatum) and its antioxidant ability after gastrointestinal digestion: relationship with constituents. Eur. J. Lipid Sci. Technol. 2015. [Google Scholar] [CrossRef]
  58. Chen, J.; Jiao, R.; Jiang, Y.; Bi, Y.; Chen, Z.Y. Algal Sterols are as effective as β-sitosterol in reducing plasma cholesterol concentration. J. Agric. Food Chem. 2014, 62, 675–681. [Google Scholar] [CrossRef] [PubMed]
  59. Spanova, M.; Daum, G. Squalene-biochemistry, molecular biology, process biotechnology, and applications. Eur. J. Lipid Sci. Technol. 2011, 113, 1299–1320. [Google Scholar] [CrossRef]
  60. Lohr, M.; Schwender, J.; Polle, J.E.W. Isoprenoid biosynthesis in eukaryotic phototrophs: A spotlight on algae. Plant Sci. 2012, 185–186, 9–22. [Google Scholar]
  61. Kim, D.; Filtz, M.R.; Proteau, P.J. The Methylerythritol phosphate pathway contributes to carotenoid but not phytol biosynthesis in Euglena gracilis. J. Nat. Prod. 2004, 67, 1067–1069. [Google Scholar] [CrossRef] [PubMed]
  62. Grauvogel, C.; Petersen, J. Isoprenoid biosynthesis authenticates the classification of the green alga Mesostigma viride as an ancient streptophyte. Gene 2007, 396, 125–133. [Google Scholar] [CrossRef] [PubMed]
  63. Disch, A.; Schwender, J.; Müller, C.; Lichtenthaler, H.K.; Rohmer, M. Distribution of the mevalonate and glyceraldehyde phosphate/pyruvate pathways for isoprenoid biosynthesis in unicellular algae and the cyanobacterium Synechocystis PCC 6714. Biochem. J. 1998, 333, 381–388. [Google Scholar] [PubMed]
  64. Lee, S.; Lee, Y.S.; Jung, S.H.; Kang, S.S.; Shin, K.H. Anti-oxidant activities of fucosterol from the marine algae Pelvetia siliquosa. Arch. Pharm. Res. 2003, 26, 719–722. [Google Scholar] [CrossRef] [PubMed]
  65. Sheu, J.H.; Wang, G.H.; Sung, P.J.; Duh, C.Y. New Cytotoxic oxygenated fucosterols from the brown alga Turbinaria conoides. J. Nat. Prod. 1999, 62, 224–227. [Google Scholar] [CrossRef] [PubMed]
  66. Choi, J.M.; Lee, E.O.; Lee, H.J.; Kim, K.H.; Ahn, K.S.; Shim, B.S.; Kim, N.I.; Song, M.C.; Baek, N.I.; Kim, S.H. Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities. Phytother. Res. 2007, 21, 954–959. [Google Scholar] [CrossRef] [PubMed]
  67. Lee, Y.S.; Shin, K.H.; Kim, B.K.; Lee, S. Anti-Diabetic activities of fucosterol from Pelvetia siliquosa. Arch. Pharm. Res. 2004, 27, 1120–1122. [Google Scholar] [CrossRef] [PubMed]
  68. Neil, H.; Meijer, G.; Roe, L. Randomised controlled trial of use by hypercholesterolaemic patients of a vegetable oil sterol-enriched fat spread. Atherosclerosis 2001, 156, 329–337. [Google Scholar] [CrossRef]
  69. Nigon, F.; Serfaty-Lacrosnière, C.; Beucler, I.; Chauvois, D.; Neveu, C.; Giral, P.; Chapman, M.J.; Bruckert, E. Plant sterol-enriched margarine lowers plasma LDL in hyperlipidemic subjects with low cholesterol intake: Effect of fibrate treatment. Clin. Chem. Lab. Med. 2001, 39, 634–640. [Google Scholar] [CrossRef] [PubMed]
  70. Bhattacharyya, A.K.; Lopez, L.A. Absorbability of plant sterols and their distribution in rabbit tissues. Biochim. Biophys. Acta 1979, 574, 146–153. [Google Scholar] [CrossRef]
  71. Paoletti, C.; Pushparaj, B.; Florenzano, G.; Capella, P.; Lercker, G. Unsaponifiable matter of green and blue-green algal lipids as a factor of biochemical differentiation of their biomasses: I. Total unsaponifiable and hydrocarbon fraction. Lipids 1976, 11, 258–265. [Google Scholar] [CrossRef]
  72. Kobori, M.; Yoshida, M.; Ohnishi-Kameyama, M.; Shinmoto, H. Ergosterol peroxide from an edible mushroom suppresses inflammatory responses in RAW264.7 macrophages and growth of HT29 colon adenocarcinoma cells. Br. J. Pharm. 2007, 150, 209–219. [Google Scholar] [CrossRef] [PubMed]
  73. Awad, A.B.; Fink, C.S. Phytosterols as Anticancer Dietary Components: Evidence and mechanism of action. J. Nutr. 2000, 130, 2127–2130. [Google Scholar] [PubMed]
  74. Khalos, K.; Kangas, L.; Hiltunen, R. Ergosterol peroxide, an active compound from Inonotus radiatus. Planta Medica 1989, 55, 389–390. [Google Scholar] [CrossRef] [PubMed]
  75. Tang, H.F.; Yi, Y.H.; Yao, X.S.; Xu, Q.Z.; Zhang, S.Y.; Lin, H.W. Bioactive steroids from the brown alga Sargassum carpophyllum. J. Asian Nat. Prod. Res. 2002, 4, 95–101. [Google Scholar] [CrossRef] [PubMed]
  76. Panda, S.; Jafri, M.; Kar, A.; Meheta, B.K. Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated from Butea monosperma. Fitoterapia 2009, 80, 123–126. [Google Scholar] [CrossRef] [PubMed]
  77. Park, P.J.; Jung, W.K.; Nam, K.S.; Shahidi, F.; Kim, S.K. Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin-free egg yolk. J. Am. Oil Chem. Soc. 2001, 78, 651–656. [Google Scholar] [CrossRef]
  78. WHO. Global Tuberculosis Report 2014; World Health Organisation: Geneva, Swizerland, 2014. [Google Scholar]
  79. Ikekawa, N.; Morisaki, N.; Tsuda, K.; Yoshida, T. Sterol compositions in some green algae and brown algae. Steroids 1968, 12, 41–48. [Google Scholar] [CrossRef]
  80. Francavilla, M.; Colaianna, M.; Zotti, M.; Morgese, M.; Trotta, P.; Tucci, P.; Schiavone, S.; Cuomo, V.; Trabace, L. Extraction, Characterization and in vivo neuromodulatory activity of phytosterols from microalga Dunaliella tertiolecta. Curr. Med. Chem. 2012, 19, 3058–3067. [Google Scholar] [CrossRef] [PubMed]
  81. Kazlowska, K.; Lin, H.T.V.; Chang, S.H.; Tasi, G.J. In vitro and in vivo anticancer effects of sterol fraction from red algae Porphyra dentata. Evid. Based Complement. Altern. Med. 2013, 2013, 10. [Google Scholar] [CrossRef] [PubMed]
  82. Yusnelti, Y.M.; Dharma, A.; Darwis, D.; Munaf, E. Steroids from N-Hexane fraction of the stem bark of shorea singkawang mig and anticancer activity as tested with Murin Leukemia P-388 cells. Res. J. Pharm. Biol. Chem. Sci. 2015, 6, 1315–1320. [Google Scholar]
  83. Mohammady, N. Different light spectral qualities influence sterol pool in Porphyridium cruentum (Rhodophyta). Am. J. Plant Physiol. 2007, 2, 115–121. [Google Scholar]
  84. Akihisa, T.; Hori, T.; Suzuki, H.; Sakoh, T.; Yokota, T.; Tamura, T. 24β-methyl-5α-cholest-9(11)-en-3β-ol, two 24β-alkyl-Δ5,7,9(11)-sterols and other 24β-alkylsterol from Chlorella vulgaris. Phytochemistry 1992, 31, 1769–1772. [Google Scholar] [CrossRef]
  85. Miller, M.B.; Haubrich, B.A.; Wang, Q.; Snell, W.J.; Nes, W.D. Evolutionarily conserved Δ25(27)-olefin ergosterol biosynthesis pathway in the alga Chlamydomonas reinhardtii. J. Lipid Res. 2012, 53, 1636–1645. [Google Scholar] [CrossRef] [PubMed]
  86. Ciminiello, P.; Fattorusso, E.; Magno, S.; Mangoni, A.; Pansini, M. Incisterols, a new class of highly degraded sterols from the marine sponge Dictyonella incisa. J. Am. Chem. Soc. 1990, 112, 3505–3509. [Google Scholar] [CrossRef]
  87. Ma, L.; Chen, H.; Dong, P.; Lu, X. Anti-inflammatory and anticancer activities of extracts and compounds from the mushroom Inonotus obliquus. Food Chem. 2013, 139, 503–508. [Google Scholar] [CrossRef] [PubMed]
  88. Wu, Q.P.; Xie, Y.Z.; Deng, Z.; Li, X.M.; Yang, W.; Jiao, C.W.; Fang, L.; Li, S.Z.; Pan, H.H.; Yee, A.J. Ergosterol peroxide isolated from Ganoderma lucidum abolishes microRNA miR-378-mediated tumor cells on chemoresistance. PLoS ONE 2012, 7, e44579. [Google Scholar] [CrossRef] [PubMed]
  89. Gil-Ramírez, A.; Caz, V.; Martin-Hernandez, R.; Marín, F.R.; Largo, C.; Rodríguez-Casado, A.; Tabernero, M.; Ruiz-Rodríguez, A.; Reglero, G.; Soler-Rivas, C. Modulation of cholesterol-related gene expression by ergosterol and ergosterol-enriched extracts obtained from Agaricus bisporus. Eur. J. Nutr. 2015, 1–17. [Google Scholar]
  90. Klosty, M.; Bergmann, W. Sterols of Algae. III. The occurrence of ergosterol in Chlorella pyranoidosa. J. Am. Chem. Soc. 1952, 74, 1601–1601. [Google Scholar] [CrossRef]
  91. Plaza, M.; Cifuentes, A.; Ibáñez, E. In the search of new functional food ingredients from algae. Trends Food Sci. Technol. 2008, 19, 31–39. [Google Scholar] [CrossRef]
  92. Kim, K.B.W.R.; Kim, M.J.; Ahn, D.H. Lipase inhibitory activity of chlorophyll a, isofucosterol and saringosterol isolated from chloroform fraction of Sargassum thunbergii. Nat. Prod. Res. 2014, 28, 1310–1312. [Google Scholar] [CrossRef] [PubMed]
  93. Wächter, G.A.; Franzblau, S.G.; Montenegro, G.; Hoffmann, J.J.; Maiese, W.M.; Timmermann, B.N. Inhibition of Mycobacterium tuberculosis growth by saringosterol from Lessonia nigrescens. J. Nat. Prod. 2001, 64, 1463–1464. [Google Scholar] [CrossRef] [PubMed]
  94. Chen, Z.; Liu, J.; Fu, Z.; Ye, C.; Zhang, R.; Song, Y.; Zhang, Y.; Li, H.; Ying, H.; Liu, H. 24(S)-Saringosterol from edible marine seaweed Sargassum fusiforme is a novel selective LXRβ agonist. J. Agric. Food Chem. 2014, 62, 6130–6137. [Google Scholar] [CrossRef] [PubMed]
  95. Awad, A.B.; Chan, K.C.; Downie, A.C.; Fink, C.S. Peanuts as a source of β-sitosterol, a sterol with anticancer properties. Nutr. Cancer 2000, 36, 238–241. [Google Scholar] [CrossRef] [PubMed]
  96. Byju, K.; Anuradha, V.; Vasundhara, G.; Nair, S.M.; Kumar, N.C. In vitro and in silico studies on the anticancer and apoptosis-inducing activities of the sterols identified from the soft coral, subergorgia reticulata. Pharmacogn. Mag. 2014, 10, S65–S71. [Google Scholar]
  97. Deepak, M.; Handa, S.S. Antiinflammatory activity and chemical composition of extracts of Verbena officinalis. Phytother. Res. 2000, 14, 463–465. [Google Scholar] [CrossRef]
  98. Villaseñor, I.M.; Angelada, J.; Canlas, A.P.; Echegoyen, D. Bioactivity studies on β-sitosterol and its glucoside. Phytother. Res. 2002, 16, 417–421. [Google Scholar] [CrossRef] [PubMed]
  99. Jamaluddin, F.; Mohamed, S.; Lajis, M.N. Hypoglycaemic effect of Parkia speciosa seeds due to the synergistic action of β-sitosterol and stigmasterol. Food Chem. 1994, 49, 339–345. [Google Scholar] [CrossRef]
  100. Gordon, M.H.; Magos, P. The effect of sterols on the oxidation of edible oils. Food Chem. 1983, 10, 141–147. [Google Scholar] [CrossRef]
  101. Karabacak, M.; Kanbur, M.; Eraslan, G.; Soyer, S.Z. The antioxidant effect of wheat germ oil on subchronic coumaphos exposure in mice. Ecotoxicol. Environ. Saf. 2011, 74, 2119–2125. [Google Scholar] [CrossRef] [PubMed]
  102. Małecka, M. Antioxidant properties of the unsaponifiable matter isolated from tomato seeds, oat grains and wheat germ oil. Food Chem. 2002, 79, 327–330. [Google Scholar] [CrossRef]
  103. Wei, W.; Yang, Y. Preparation of two phytosterols from rape bee pollen and determination by HPLC-ELSD. Chin. J. Pharm. 2011, 6, 008. [Google Scholar]
  104. Giner, J.L.; Gunasekera, S.P.; Pomponi, S.A. Sterols of the marine sponge Petrosia weinbergi: Implications for the absolute configurations of the antiviral orthoesterols and weinbersterols. Steroids 1999, 64, 820–824. [Google Scholar] [CrossRef]
  105. Demonty, I.; Haddeman, E.; van der Put, N.; Duchateau, G.; Steenbergen, H.; Diks, R.; Trautwein, E. Spreads fortified with a brassicasterol-rich phytosterol mixture from rapeseed oil lower serum total and LDL-cholesterol concentrations in mildly hypercholesterolemic subjects. J. Fed. Am. Soc. Exp. Biol. 2007, 21, A1089. [Google Scholar]
  106. Napolitano, G.E.; Ackman, R.G.; Silva-Serra, M.A. Incorporation of dietary sterols by the sea scallop Placopecten magellanicus (Gmelin) fed on microalgae. Mar. Biol. 1993, 117, 647–654. [Google Scholar] [CrossRef]
  107. Chu, F.L.E.; Lund, E.D.; Littreal, P.R.; Ruck, K.E.; Harvey, E.; Le Coz, J.; Marty, Y.; Moal, J.; Soudant, P. Sterol production and phytosterol bioconversion in two species of heterotrophic protists, Oxyrrhis marina and Gyrodinium dominans. Mar. Biol. 2008, 156, 155–169. [Google Scholar] [CrossRef]
  108. Ho, D.S.S. Recovery of Phytonutrients from Oils. EP Patent 1689353 B1, 9 January 2005. [Google Scholar]
  109. Smith, F.E. Separation of Tocopherols and Sterols from Deodorizer Sludge and the Like. U.S. Patent 3335154 A, 8 August 1967. [Google Scholar]
  110. Fizet, C. Process for Tocopherols and Sterols from Natural Sources. U.S. Patent 5487817 A, 30 January 1996. [Google Scholar]
  111. Huibers, D.T.A.; Robbins, A.M.; Sullivan, D.H. Method for Separating Sterols from Tall Oil. U.S. Patent 6107456, 22 August 2000. [Google Scholar]
  112. Mouahid, A.; Crampon, C.; Toudji, S.A.; Badens, E. Supercritical CO2 extraction of neutral lipids from microalgae: Experiments and modelling. J. Supercrit. Fluids 2013, 77, 7–16. [Google Scholar]
  113. Snyder, J.M.; King, J.W.; Taylor, S.L.; Neese, A.L. Concentration of phytosterols for analysis by supercritical fluid extraction. J. Am. Oil Chem. Soc. 1999, 76, 717–721. [Google Scholar] [CrossRef]
  114. Dunford, N.T.; King, J.W. Phytosterol Enrichment of Rice Bran Oil by a Supercritical Carbon Dioxide Fractionation Technique. J. Food Sci. 2000, 65, 1395–1399. [Google Scholar] [CrossRef]
  115. Nyam, K.L.; Tan, C.P.; Lai, O.M.; Long, K.; Che, M.Y.B. Optimization of supercritical fluid extraction of phytosterol from roselle seeds with a central composite design model. Food Bioprod. Process. 2010, 88, 239–246. [Google Scholar] [CrossRef]
  116. Sajfrtová, M.; Lickova, I.; Wimmerova, M.; Sovova, H.; Wimmer, Z. β-Sitosterol: Supercritical carbon dioxide extraction from sea buckthorn (Hippophae rhamnoides L.) seeds. Int. J. Mol. Sci. 2010, 11, 1842–1850. [Google Scholar] [CrossRef] [PubMed]
  117. Herrero, M.; Cifuentes, A.; Ibañez, E. Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae: A review. Food Chem. 2006, 98, 136–148. [Google Scholar] [CrossRef]
  118. Capuzzo, A.; Maffei, M.; Occhipinti, A. Supercritical fluid extraction of plant flavors and fragrances. Molecules 2013, 18, 7194–7238. [Google Scholar] [CrossRef] [PubMed]
  119. Wang, L.; Weller, C.L. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Technol. 2006, 17, 300–312. [Google Scholar] [CrossRef]
  120. Uddin, M.S.; Sarker, M.Z.I.; Ferdosh, S.; Akanda, M.J.H.; Easmin, M.S.; Bt Shamsudin, S.H.; Yunus, K.B. Phytosterols and their extraction from various plant matrices using supercritical carbon dioxide: A review. J. Sci. Food Agric. 2015, 95, 1385–1394. [Google Scholar] [CrossRef] [PubMed]
  121. Chen, M.; Liu, T.; Chen, X.; Chen, L.; Zhang, W.; Wang, J.; Gao, L.; Chen, Y.; Peng, X. Subcritical co-solvents extraction of lipid from wet microalgae pastes of Nannochloropsis sp. Eur. J. Lipid Sci. Technol. 2012, 114, 205–212. [Google Scholar] [CrossRef] [PubMed]
  122. Dejoye, C.; Vian, M.A.; Lumia, G.; Bouscarle, C.; Charton, F.; Chemat, F. Combined extraction processes of lipid from Chlorella vulgaris microalgae: Microwave prior to supercritical carbon dioxide extraction. Int. J. Mol. Sci. 2011, 12, 9332–9341. [Google Scholar] [CrossRef] [PubMed]
  123. Ktari, L.; Blond, A.; Guyot, M. 16β-Hydroxy-5α-cholestane-3,6-dione, a novel cytotoxic oxysterol from the red alga Jania rubens. Bioorganic Med. Chem. Lett. 2000, 10, 2563–2565. [Google Scholar] [CrossRef]
  124. Sheu, J.H.; Wang, G.H.; Sung, P.J.; Chiu, Y.H.; Duh, C.Y. Cytotoxic sterols from the formosan brown alga Turbinaria ornata. Planta Medica 1997, 63, 571–572. [Google Scholar] [CrossRef] [PubMed]
  125. Lin, A.S.; Engel, S.; Smith, B.A.; Fairchild, C.R.; Aalbersberg, W.; Hay, M.E.; Kubanek, J. Structure and biological evaluation of novel cytotoxic sterol glycosides from the marine red alga Peyssonnelia sp. Bioorganic Med. Chem. 2010, 18, 8264–8269. [Google Scholar] [CrossRef] [PubMed]
  126. Nes, W.D. Biosynthesis of cholesterol and other sterols. Chem. Rev. 2011, 111, 6423–6451. [Google Scholar] [CrossRef] [PubMed]
  127. Chen, J.; Jiang, Y.; Liang, Y.; Tian, X.; Peng, C.; Ma, K.Y.; Liu, J.; Huang, Y.; Chen, Z.Y. DPA n-3, DPA n-6 and DHA improve lipoprotein profiles and aortic function in hamsters fed a high cholesterol diet. Atherosclerosis 2012, 221, 397–404. [Google Scholar] [CrossRef] [PubMed]
  128. Pistocchi, R.; Guerrini, F.; Pezzolesi, L.; Riccardi, M.; Vanucci, S.; Ciminiello, P.; Dell’Aversano, C.; Forino, M.; Fattorusso, E.; Tartaglione, L. Toxin levels and profiles in microalgae from the North-Western Adriatic Sea—15 years of studies on cultured species. Mar. Drugs 2012, 10, 140–162. [Google Scholar] [CrossRef] [PubMed]
  129. Dorantes-Aranda, J.J.; Waite, T.D.; Godrant, A.; Rose, A.L.; Tovar, C.D.; Woods, G.M.; Hallegraeff, G.M. Novel application of a fish gill cell line assay to assess ichthyotoxicity of harmful marine microalgae. Harmful Algae 2011, 10, 366–373. [Google Scholar] [CrossRef]
  130. Alemany, L.; Barbera, R.; Alegria, A.; Laparra, J.M. Plant sterols from foods in inflammation and risk of cardiovascular disease: A real threat? Food Chem. Toxicol. 2014, 69, 140–149. [Google Scholar] [CrossRef] [PubMed]
  131. González-Larena, M.; Garcia-Llatas, G.; Clemente, G.; Barbera, R.; Lagarda, M.J. Plant sterol oxides in functional beverages: Influence of matrix and storage. Food Chem. 2015, 173, 881–889. [Google Scholar] [CrossRef] [PubMed]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top