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Open AccessArticle

The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2

1
Blue-Bio Industry RIC and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea
2
Department of Microbiology, College of Medicine, Inje University, Busan 608-756, Korea
3
Marine Biodiversity Institute of Korea, Seocheon 325-902, Korea
4
Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea
5
Department of Food and Nutrition, College of Natural Sciences & Human Ecology, Dongeui University, Busan 614-714, Korea
6
Department of Life Science and Biotechnology, College of Natural Sciences & Human Ecology, Dongeui University, Busan 614-714, Korea
7
Department of Biochemistry, Busan National University College of Medicine, Yangsan 626-870, Korea
8
Department of Anatomy and Cell Biology, Dong-A University College of Medicine & Mitochondria Hub Regulation Center, Busan 602-714, Korea
9
Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 614-052, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Colin Barrow
Mar. Drugs 2015, 13(5), 2666-2679; https://doi.org/10.3390/md13052666
Received: 13 March 2015 / Revised: 8 April 2015 / Accepted: 21 April 2015 / Published: 29 April 2015
(This article belongs to the Special Issue Marine Functional Food)
This study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H2O2)-induced growth inhibition and exhibited scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2 in mouse-derived C2C12 myoblasts. PBME also significantly attenuated H2O2-induced comet tail formation in a comet assay, histone γH2A.X phosphorylation, and annexin V-positive cells, suggesting that PBME prevented H2O2-induced cellular DNA damage and apoptotic cell death. Furthermore, PBME increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a HO-1 competitive inhibitor, significantly abolished the protective effects of PBME on H2O2-induced ROS generation, growth inhibition, and apoptosis. Collectively, these results demonstrate that PBME augments the antioxidant defense capacity through activation of the Nrf2/HO-1 pathway. View Full-Text
Keywords: Petalonia binghamiae; oxidative stress; ROS; DNA damage; Nrf2/HO-1 Petalonia binghamiae; oxidative stress; ROS; DNA damage; Nrf2/HO-1
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Kang, J.S.; Choi, I.-W.; Han, M.H.; Lee, D.-S.; Kim, G.-Y.; Hwang, H.J.; Kim, B.W.; Kim, C.M.; Yoo, Y.H.; Choi, Y.H. The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2. Mar. Drugs 2015, 13, 2666-2679.

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