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Correction published on 5 September 2014, see Mar. Drugs 2014, 12(9), 4741-4742.
Article

Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16

1
Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, Lublin 20-033, Poland
2
Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c, Borstel D-23845, Germany
3
Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, Borstel D-23845, Germany
4
Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, Pulawy 24-100, Poland
*
Author to whom correspondence should be addressed.
Mar. Drugs 2014, 12(3), 1298-1316; https://doi.org/10.3390/md12031298
Received: 24 December 2013 / Revised: 27 January 2014 / Accepted: 8 February 2014 / Published: 7 March 2014
(This article belongs to the Special Issue Marine Lipopolysaccharides)
Chemical analyses, mass spectrometry, and NMR spectroscopy were applied to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas veronii strain Bs19, serotype O16. ESI-MS revealed that the most abundant LPS glycoforms have tetra-acylated or hexa-acylated lipid A species, consisting of a bisphosphorylated GlcN disaccharide with an AraN residue as a non-stoichiometric substituent, and a core oligosaccharide composed of Hep5Hex3HexN1Kdo1P1. Sugar and methylation analysis together with 1D and 2D 1H and 13C NMR spectroscopy were the main methods used, and revealed that the O-specific polysaccharide (OPS) of A. veronii Bs19 was built up of tetrasaccharide repeating units with the structure: →4)-α-d-Quip3NAc-(1→3)-α-l-Rhap-(1→4)-β-d-Galp-(1→3)-α-d-GalpNAc-(1→. This composition was confirmed by mass spectrometry. The charge-deconvoluted ESI FT-ICR MS recorded for the LPS preparations identified mass peaks of SR- and R-form LPS species, that differed by Δm = 698.27 u, a value corresponding to the calculated molecular mass of one OPS repeating unit (6dHexNAc6dHexHexHexNAc-H2O). Moreover, unspecific fragmentation spectra confirmed the sequence of the sugar residues in the OPS and allowed to assume that the elucidated structure also represented the biological repeating unit. View Full-Text
Keywords: lipopolysaccharide; O-specific polysaccharide; Aeromonas veronii; fish pathogen; ESI MS; NMR lipopolysaccharide; O-specific polysaccharide; Aeromonas veronii; fish pathogen; ESI MS; NMR
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MDPI and ACS Style

Turska-Szewczuk, A.; Duda, K.A.; Schwudke, D.; Pekala, A.; Kozinska, A.; Holst, O. Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16. Mar. Drugs 2014, 12, 1298-1316. https://doi.org/10.3390/md12031298

AMA Style

Turska-Szewczuk A, Duda KA, Schwudke D, Pekala A, Kozinska A, Holst O. Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16. Marine Drugs. 2014; 12(3):1298-1316. https://doi.org/10.3390/md12031298

Chicago/Turabian Style

Turska-Szewczuk, Anna, Katarzyna A. Duda, Dominik Schwudke, Agnieszka Pekala, Alicja Kozinska, and Otto Holst. 2014. "Structural Studies of the Lipopolysaccharide from the Fish Pathogen Aeromonas veronii Strain Bs19, Serotype O16" Marine Drugs 12, no. 3: 1298-1316. https://doi.org/10.3390/md12031298

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