Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae
Abstract
:1. Introduction
2. Results and Discussion
2.1. C816 Is More Potent on S. cerevisiae than C800 and C830, and Has a Fungicidal Effect
2.2. C816 Induces Cell Cycle Arrest and an Increase in S. cerevisiae DNA Content
2.3. C816 Induces an Increase in Cell Size
2.4. C816 Induces Apoptosis in S. cerevisiae
2.5. C816 Disrupts the Mitochondrial Membrane Potential (ΔΨm) in S. cerevisiae
3. Experimental Section
3.1. Molecules Tested
3.2. Cell Strain and Culture
3.3. Cell Growth Inhibition Determination
3.4. Determination of Fungicidal Effect
3.5. Cell Cycle Assay by Flow Cytometry
3.6. Determination of Apoptosis by Annexin V and Propidium Iodide (PI) Staining
3.7. Determination of Mitochondrial Transmembrane Potential (ΔΨm)
3.8. Statistics
4. Conclusion
Acknowledgments
Conflicts of Interest
References
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Rubiolo, J.A.; Ternon, E.; López-Alonso, H.; Thomas, O.P.; Vega, F.V.; Vieytes, M.R.; Botana, L.M. Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae. Mar. Drugs 2013, 11, 4419-4434. https://doi.org/10.3390/md11114419
Rubiolo JA, Ternon E, López-Alonso H, Thomas OP, Vega FV, Vieytes MR, Botana LM. Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae. Marine Drugs. 2013; 11(11):4419-4434. https://doi.org/10.3390/md11114419
Chicago/Turabian StyleRubiolo, Juan A., Eva Ternon, Henar López-Alonso, Olivier P. Thomas, Félix V. Vega, Mercedes R. Vieytes, and Luis M. Botana. 2013. "Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae" Marine Drugs 11, no. 11: 4419-4434. https://doi.org/10.3390/md11114419