Dynamical Behavior of Disordered Regions in Disease-Related Proteins Revealed by Quasielastic Neutron Scattering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
3. Results
3.1. Analysis of the QENS Spectra of the Tn-CD
3.2. Analysis of the QENS Spectra of αSyn
4. Discussion
5. Conclusions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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wtTn-CD Low Ca2+ | wtTn-CD High Ca2+ | mtTn-CD Low Ca2+ | mtTn-CD High Ca2+ | αSyn Low Salt | αSyn High Salt | |
---|---|---|---|---|---|---|
DT (×10−7 cm2/s) | 4.64 ± 0.01 | 4.68 ± 0.02 | 4.79 ± 0.02 | 4.87 ± 0.02 | 5.59 ± 0.03 | 4.70 ± 0.04 |
DR (×106 1/s) | 1.62 ± 0.13 | 1.64 ± 0.17 | 1.76 ± 0.16 | 1.86 ± 0.18 | 2.54 ± 0.03 | 1.63 ± 0.06 |
Dapp (×10−7 cm2/s) | Dtrans+rot (×10−7 cm2/s) | Dseg (×10−7 cm2/s) | |
---|---|---|---|
wtTn-CD, Low-Ca2+ state | 8.02 ± 0.15 | 5.92 ± 0.02 | 2.28 ± 0.16 |
wtTn-CD, High-Ca2+ state | 8.24 ± 0.14 | 5.99 ± 0.02 | 2.43 ± 0.15 |
mtTn-CD, Low-Ca2+ state | 8.24 ± 0.14 | 6.09 ± 0.02 | 2.39 ± 0.16 |
mtTn-CD, High-Ca2+ state | 8.18 ± 0.14 | 6.22 ± 0.02 | 2.02 ± 0.15 |
Dapp (×10−7 cm2/s) | Dtrans+rot (×10−7 cm2/s) | Dseg (×10−7 cm2/s) | |
---|---|---|---|
Low salt | 20.3 ± 1.1 | 6.88 ± 0.01 | 14.3 ± 0.9 |
High salt | 21.7 ± 0.9 | 6.49 ± 0.02 | 15.1 ± 0.8 |
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Fujiwara, S. Dynamical Behavior of Disordered Regions in Disease-Related Proteins Revealed by Quasielastic Neutron Scattering. Medicina 2022, 58, 795. https://doi.org/10.3390/medicina58060795
Fujiwara S. Dynamical Behavior of Disordered Regions in Disease-Related Proteins Revealed by Quasielastic Neutron Scattering. Medicina. 2022; 58(6):795. https://doi.org/10.3390/medicina58060795
Chicago/Turabian StyleFujiwara, Satoru. 2022. "Dynamical Behavior of Disordered Regions in Disease-Related Proteins Revealed by Quasielastic Neutron Scattering" Medicina 58, no. 6: 795. https://doi.org/10.3390/medicina58060795