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Article

In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin

by
Gabriel Veniamin Cozma
1,†,
Alexandru Apostu
2,3,†,
Ioana Macasoi
4,5,*,
Cristina Adriana Dehelean
4,5,*,
Octavian Marius Cretu
6,
Stefania Dinu
7,8,
Dan Gaiță
2,3 and
Aniko Manea
9
1
Department of Surgical Semiology, Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
2
Department of Cardiology, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 49 No., C. D. Loga Bv., 300041 Timişoara, Romania
3
Advanced Research Center of the Institute for Cardiovascular Diseases, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
4
Departament of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
5
Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
6
Department of Surgery, Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
7
Department of Pedodontics, Faculty of Dental Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania
8
Pediatric Dentistry Research Center, Faculty of Dental Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania
9
Department of Obstetrics and Gynecology, Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Medicina 2022, 58(6), 705; https://doi.org/10.3390/medicina58060705
Submission received: 21 April 2022 / Revised: 21 May 2022 / Accepted: 24 May 2022 / Published: 25 May 2022
(This article belongs to the Section Gastroenterology & Hepatology)
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Abstract

Background and Objectives: Metformin is currently the leading drug of choice for treating type 2 diabetes mellitus, being one of the most widely used drugs worldwide. The beneficial effects of Metformin, however, extend far beyond the reduction of blood glucose. Therefore, this study aimed to evaluate Metformin’s effects both in vitro and in ovo. Materials and Methods: Metformin has been tested in five different concentrations in human hepatocytes —HepaRG, in terms of cell viability, morphology, structure and number of nuclei and mitochondria, as well as the effect on cell migration. Through the application of HET-CAM, the biocompatibility and potential anti-irritant, as well as protective effects on the vascular plexus were also assessed. Results: According to the results obtained, Metformin increases cell viability without causing morphological changes to cells, mitochondria, or nuclei. Metformin displayed an anti-irritant activity rather than causing irritation at the level of the vascular plexus. Conclusions: In conclusion, Metformin enhances cell viability and proliferation and, has a protective effect on the vascular plexus. Nonetheless, more studies are required to clarify the mechanism of hepatoprotective effect of metformin.
Keywords: metformin; hepatoprotective; anti-irritant; chorioallantoic membrane; HET-CAM metformin; hepatoprotective; anti-irritant; chorioallantoic membrane; HET-CAM

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MDPI and ACS Style

Cozma, G.V.; Apostu, A.; Macasoi, I.; Dehelean, C.A.; Cretu, O.M.; Dinu, S.; Gaiță, D.; Manea, A. In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin. Medicina 2022, 58, 705. https://doi.org/10.3390/medicina58060705

AMA Style

Cozma GV, Apostu A, Macasoi I, Dehelean CA, Cretu OM, Dinu S, Gaiță D, Manea A. In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin. Medicina. 2022; 58(6):705. https://doi.org/10.3390/medicina58060705

Chicago/Turabian Style

Cozma, Gabriel Veniamin, Alexandru Apostu, Ioana Macasoi, Cristina Adriana Dehelean, Octavian Marius Cretu, Stefania Dinu, Dan Gaiță, and Aniko Manea. 2022. "In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin" Medicina 58, no. 6: 705. https://doi.org/10.3390/medicina58060705

APA Style

Cozma, G. V., Apostu, A., Macasoi, I., Dehelean, C. A., Cretu, O. M., Dinu, S., Gaiță, D., & Manea, A. (2022). In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin. Medicina, 58(6), 705. https://doi.org/10.3390/medicina58060705

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