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Medicina
Volume 58
Issue 2
10.3390/medicina58020280
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Communication

St. Thomas Modified Cardioplegia Effects on Myoblasts’ Viability and Morphology

by
Rafał Nowicki
1,*,
Katarzyna Bieżuńska-Kusiak
2,
Julita Kulbacka
2,
Anna Choromanska
2,
Małgorzata Daczewska
3,
Stanisław Potoczek
4,
Maciej Rachwalik
1 and
Jolanta Saczko
2
1
Department of Cardiac Surgery, Wroclaw Medical University, Curie-Skłodowskiej, 50-369 Wrocław, Poland
2
Department of Molecular and Cellular Biology, Wroclaw Medical University, Borowska 211A, 50-368 Wrocław, Poland
3
Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21 St., 50-335 Wrocław, Poland
4
Department of Hematology, Blood Neoplasms and Bone Marrow Transplantation, Wroclaw Medical University, Wybrzeże Pasteura 4, 50-367 Wrocław, Poland
*
Author to whom correspondence should be addressed.
Medicina 2022, 58(2), 280; https://doi.org/10.3390/medicina58020280
Submission received: 28 December 2021 / Revised: 2 February 2022 / Accepted: 11 February 2022 / Published: 13 February 2022
(This article belongs to the Special Issue Strategies and Concepts of Extracorporeal Life Support)
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Abstract

Background and Objectives: The cardioplegic arrest of the heart during cardiosurgical procedures is the crucial element of a cardioprotection strategy. Numerous clinical trials compare different cardioplegic solutions and cardioprotective protocols, but a relatively small number of papers apply to in vitro conditions using cultured cells. This work aimed to analyze whether it is possible to use the rat heart myocardium cells as an in vitro model to study the protective properties of St. Thomas cardioplegia (ST2C). Methods: The rat heart myocardium cells-H9C2 were incubated with cold cardioplegia for up to 24 h. After incubation, we determined: viability, confluency, and cell size, the thiol groups’ level by modifying Ellman’s method, Ki67, and Proliferating Cell Nuclear Antigen expression (PCNA). The impact on cells’ morphology was visualized by the ultrastructural (TEM) study and holotomograpic 3D imaging. Results: The viability and confluency analysis demonstrated that the safest exposure to ST2C, should not exceed 4h. An increased expression of Ki67 antigen and PCNA was observed. TEM and 3D imaging studies revealed vacuolization after the longest period of exposure (24). Conclusions: According to obtained results, we conclude that STC can play a protective role in cardiac surgery during heart arrest.
Keywords: rat heart myocardium cells; proliferating antigens; thiol groups; cold crystalloid cardioplegia rat heart myocardium cells; proliferating antigens; thiol groups; cold crystalloid cardioplegia

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MDPI and ACS Style

Nowicki, R.; Bieżuńska-Kusiak, K.; Kulbacka, J.; Choromanska, A.; Daczewska, M.; Potoczek, S.; Rachwalik, M.; Saczko, J. St. Thomas Modified Cardioplegia Effects on Myoblasts’ Viability and Morphology. Medicina 2022, 58, 280. https://doi.org/10.3390/medicina58020280

AMA Style

Nowicki R, Bieżuńska-Kusiak K, Kulbacka J, Choromanska A, Daczewska M, Potoczek S, Rachwalik M, Saczko J. St. Thomas Modified Cardioplegia Effects on Myoblasts’ Viability and Morphology. Medicina. 2022; 58(2):280. https://doi.org/10.3390/medicina58020280

Chicago/Turabian Style

Nowicki, Rafał, Katarzyna Bieżuńska-Kusiak, Julita Kulbacka, Anna Choromanska, Małgorzata Daczewska, Stanisław Potoczek, Maciej Rachwalik, and Jolanta Saczko. 2022. "St. Thomas Modified Cardioplegia Effects on Myoblasts’ Viability and Morphology" Medicina 58, no. 2: 280. https://doi.org/10.3390/medicina58020280

APA Style

Nowicki, R., Bieżuńska-Kusiak, K., Kulbacka, J., Choromanska, A., Daczewska, M., Potoczek, S., Rachwalik, M., & Saczko, J. (2022). St. Thomas Modified Cardioplegia Effects on Myoblasts’ Viability and Morphology. Medicina, 58(2), 280. https://doi.org/10.3390/medicina58020280

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