Next Article in Journal
Platelets in Fetal Growth Restriction: Role of Reactive Oxygen Species, Oxygen Metabolism, and Aggregation
Next Article in Special Issue
An Intrinsic Host Defense against HSV-1 Relies on the Activation of Xenophagy with the Active Clearance of Autophagic Receptors
Previous Article in Journal
Response of Astrocyte Subpopulations Following Spinal Cord Injury
Previous Article in Special Issue
The Role of Mitophagy in Viral Infection
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Cells
Volume 11
Issue 4
10.3390/cells11040723
cells-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Cannabinoids Reduce Extracellular Vesicle Release from HIV-1 Infected Myeloid Cells and Inhibit Viral Transcription

by
Catherine DeMarino
1,†,
Maria Cowen
1,†,
Pooja Khatkar
1,†,
Bianca Cotto
2,
Heather Branscome
1,
Yuriy Kim
1,
Sarah Al Sharif
3,
Emmanuel T. Agbottah
4,
Weidong Zhou
5,
Cecilia T. Costiniuk
6,7,
Mohammad-Ali Jenabian
8,
Cohava Gelber
9,
Lance A. Liotta
5,
Dianne Langford
2 and
Fatah Kashanchi
1,*
1
Laboratory of Molecular Virology, School of Systems Biology, George Mason University, Manassas, VA 22030, USA
2
Department of Neuroscience, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA
3
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud Bin Abdulaziz, University for Health Sciences, Jeddah 22384, Saudi Arabia
4
Montgomery College, Takoma Park, MD 20912, USA
5
Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA 20110, USA
6
Infectious Diseases and Immunity in Global Health Program, Research Institute of McGill University Health Centre, Montreal, QC H4A 3J1, Canada
7
Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada
8
Department of Biological Sciences and CERMO-FC Research Centre, Université du Québec à Montréal, Montreal, QC H3C 3J7, Canada
9
Serpin Pharma, Manassas, VA 20109, USA
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2022, 11(4), 723; https://doi.org/10.3390/cells11040723
Submission received: 26 January 2022 / Revised: 12 February 2022 / Accepted: 16 February 2022 / Published: 18 February 2022
(This article belongs to the Collection Role of Autophagy in Viral Infection)
Browse Figures
Versions Notes

Abstract

Of the 37.9 million individuals infected with human immunodeficiency virus type 1 (HIV-1), approximately 50% exhibit HIV-associated neurocognitive disorders (HAND). We and others previously showed that HIV-1 viral RNAs, such as trans-activating response (TAR) RNA, are incorporated into extracellular vesicles (EVs) and elicit an inflammatory response in recipient naïve cells. Cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC), the primary cannabinoids present in cannabis, are effective in reducing inflammation. Studies show that cannabis use in people living with HIV-1 is associated with lower viral load, lower circulating CD16+ monocytes and high CD4+ T-cell counts, suggesting a potentially therapeutic application. Here, HIV-1 infected U1 monocytes and primary macrophages were used to assess the effects of CBD. Post-CBD treatment, EV concentrations were analyzed using nanoparticle tracking analysis. Changes in intracellular and EV-associated viral RNA were quantified using RT-qPCR, and changes in viral proteins, EV markers, and autophagy proteins were assessed by Western blot. Our data suggest that CBD significantly reduces the number of EVs released from infected cells and that this may be mediated by reducing viral transcription and autophagy activation. Therefore, CBD may exert a protective effect by alleviating the pathogenic effects of EVs in HIV-1 and CNS-related infections.
Keywords: human immunodeficiency virus (HIV); cannabinoid; autophagy; extracellular vesicles (EVs); transcription human immunodeficiency virus (HIV); cannabinoid; autophagy; extracellular vesicles (EVs); transcription

Share and Cite

MDPI and ACS Style

DeMarino, C.; Cowen, M.; Khatkar, P.; Cotto, B.; Branscome, H.; Kim, Y.; Sharif, S.A.; Agbottah, E.T.; Zhou, W.; Costiniuk, C.T.; et al. Cannabinoids Reduce Extracellular Vesicle Release from HIV-1 Infected Myeloid Cells and Inhibit Viral Transcription. Cells 2022, 11, 723. https://doi.org/10.3390/cells11040723

AMA Style

DeMarino C, Cowen M, Khatkar P, Cotto B, Branscome H, Kim Y, Sharif SA, Agbottah ET, Zhou W, Costiniuk CT, et al. Cannabinoids Reduce Extracellular Vesicle Release from HIV-1 Infected Myeloid Cells and Inhibit Viral Transcription. Cells. 2022; 11(4):723. https://doi.org/10.3390/cells11040723

Chicago/Turabian Style

DeMarino, Catherine, Maria Cowen, Pooja Khatkar, Bianca Cotto, Heather Branscome, Yuriy Kim, Sarah Al Sharif, Emmanuel T. Agbottah, Weidong Zhou, Cecilia T. Costiniuk, and et al. 2022. "Cannabinoids Reduce Extracellular Vesicle Release from HIV-1 Infected Myeloid Cells and Inhibit Viral Transcription" Cells 11, no. 4: 723. https://doi.org/10.3390/cells11040723

APA Style

DeMarino, C., Cowen, M., Khatkar, P., Cotto, B., Branscome, H., Kim, Y., Sharif, S. A., Agbottah, E. T., Zhou, W., Costiniuk, C. T., Jenabian, M.-A., Gelber, C., Liotta, L. A., Langford, D., & Kashanchi, F. (2022). Cannabinoids Reduce Extracellular Vesicle Release from HIV-1 Infected Myeloid Cells and Inhibit Viral Transcription. Cells, 11(4), 723. https://doi.org/10.3390/cells11040723

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop