Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of Lactobacillus iners Derived from Korean Women’s Skin in Their Twenties
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. 16S rRNA Gene Sequence and Phylogenetic Analysis
2.3. Preparation of Lactobacillus Iners KOLBM20 Lysate
2.4. GC-MS Analysis of Extracellular Metabolites
2.5. Cell Cultures and Viability Assay
2.6. Ultraviolet Irradiation
2.7. Cytotoxicity
2.8. Enzyme-Linked Immunosorbent Assay (ELISA) for MMP-1 and Type I Collagen Expression
2.9. Western Blotting
2.10. Statistical Analysis
3. Results
3.1. 16S rRNA Gene Sequence and Phylogenetic Analysis
3.2. Metabolomics Analysis by GC-MS
3.3. Inhibitory Effect of Strain KOLBM20 Lysate on MMP-1 Secretion and Type I Procollagen Degradation in UVB-Stimulated Dermal Fibroblasts
3.4. The MMP-1 Inhibition by Strain KOLBM20 Lysate through the Suppression of Signalling Pathway
4. Discussion
5. Conclusions
- Strain KOLBM20 was identified as a member of the Lactobacillus genus and found to have a close genetic affinity with L. iners DSM13335(T), sharing a 99.20% similarity in 16S rRNA gene sequences.
- We also confirmed the anti-aging properties of strain KOLBM20 lysate, specifically its potential to inhibit MMP-1 expression.
- While previous research has shown that Lactobacillus species can stimulate dermal fibroblast proliferation and enhance extracellular matrix deposition in vitro, the specific impact of Lactobacillus iners strain KOLBM20 on MMP-1 expression had not been previously explored.
- Our research revealed that strain KOLBM20 lysate effectively reduced the expression of MMP-1 induced by UVB irradiation in in vitro experiments. This inhibitory effect persisted over time, possibly due to the presence of uracil in KOLBM20, a compound known to inhibit MMP-1 expression.
- We identified the involvement of the ERK and JNK signaling pathways in UVB-induced MMP-1 expression. Strain KOLBM20 lysate was found to suppress these pathways, suggesting a potential mechanism for its inhibition of MMP-1 expression.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lee, J.-S.; Min, J.-W.; Gye, S.-B.; Kim, Y.-W.; Kang, H.-C.; Choi, Y.-S.; Seo, W.-S.; Lee, B.-Y. Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of Lactobacillus iners Derived from Korean Women’s Skin in Their Twenties. Curr. Issues Mol. Biol. 2024, 46, 513-526. https://doi.org/10.3390/cimb46010033
Lee J-S, Min J-W, Gye S-B, Kim Y-W, Kang H-C, Choi Y-S, Seo W-S, Lee B-Y. Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of Lactobacillus iners Derived from Korean Women’s Skin in Their Twenties. Current Issues in Molecular Biology. 2024; 46(1):513-526. https://doi.org/10.3390/cimb46010033
Chicago/Turabian StyleLee, Jin-Sung, Jin-Woo Min, Seong-Bong Gye, Yong-Woo Kim, Hee-Cheol Kang, Yoon-Seo Choi, Won-Sang Seo, and Bun-Yeoul Lee. 2024. "Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of Lactobacillus iners Derived from Korean Women’s Skin in Their Twenties" Current Issues in Molecular Biology 46, no. 1: 513-526. https://doi.org/10.3390/cimb46010033
APA StyleLee, J.-S., Min, J.-W., Gye, S.-B., Kim, Y.-W., Kang, H.-C., Choi, Y.-S., Seo, W.-S., & Lee, B.-Y. (2024). Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of Lactobacillus iners Derived from Korean Women’s Skin in Their Twenties. Current Issues in Molecular Biology, 46(1), 513-526. https://doi.org/10.3390/cimb46010033