HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil
Abstract
:1. Introduction
2. Materials and Methods
2.1. DNA Extraction and PCR Amplification
2.2. Sanger Sequencing
2.3. Data Analysis
3. Results
3.1. Association of HLA-G and LILRB1/2 Polymorphisms in Similar/Dissimilar Groups
3.2. Association of HLA-G and LILRB1/2 Polymorphisms in the ZIKV-Transmitting and Non-Transmitting Mothers and ZIKV-Infected and Non-Infected Children
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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MOTHERS | |||||
---|---|---|---|---|---|
ALLELES/GENOTYPES | M-ZIKV− | M-ZIKV+ | OR (CI) | p-Value | Pc |
N = 6 | N = 13 † | ||||
LILRB1_rs1061684 C > T | |||||
n = 6 (%) | n = 12 (%) | ||||
C/T | 5 (83) | 3 (25) | 12.4 (1.91–391) | 0.03 | 0.06 |
T/T | 1 (17) | 9 (75) | |||
LILRB1_rs16985478 G > A | |||||
n = 6 (%) | n = 12 (%) | ||||
G | 5 (42) | 2 (8) | 7.3 (1.18–64.9) | 0.03 | 0.06 |
A | 7 (58) | 22 (92) | |||
G/A | 5 (83) | 2 (17) | 19.2 (1.71–643) | 0.01 | 0.02 |
A/A | 1 (17) | 10 (83) | |||
LILRB2_rs386056 A > G | |||||
n = 6 (%) | n = 11 (%) | ||||
A/A | 0 | 1 (9) | 0.07 (0.0–0.95) 1 | 0.02 | 0.08 |
G/A | 5 (83) | 3 (27) | |||
G/G | 1 (17) | 7 (64) | |||
HLAG_rs1710 C > G | |||||
n = 6 (%) | n = 13 (%) | ||||
C | 8 (67) | 8 (31) | 4.30 (1.005–20.9) | 0.04 | 0.52 |
G | 4 (33) | 18 (69) | |||
HLAG_rs9380142 T > C | |||||
n = 6 (%) | n = 13 (%) | ||||
T/T | 1 (17) | 7 (54) | 0.14 (0.01–1.32) 2 | 0.04 | 0.52 |
T/C | 4 (67) | 6 (46) | |||
C/C | 1 (17) | 0 |
CHILDREN | |||||
---|---|---|---|---|---|
ALLELES/GENOTYPES | C-ZIKV− | C-ZIKV+ | OR (CI) | p-Value | Pc |
N = 6 | N = 14 **† | ||||
LILRB1_rs1061684 C > T | |||||
n = 6 (%) | n = 13 (%) | ||||
T | 6 (50) | 23 (88) | 7.16 (1.38–44.6) | 0.01 | 0.02 |
C | 6 (50) | 3 (12) | |||
C/T | 6 (100) | 3 (23) | 16.4 (1.8–471) | 0.008 | 0.016 |
T/T | 0 | 10 (77) | |||
LILRB1_rs16985478 G > A | |||||
n = 6 (%) | n = 13 (%) | ||||
G | 6 (50) | 3 (12) | 7.16 (1.38–44.6) | 0.01 | 0.02 |
A | 6 (50) | 23 (88) | |||
G/A | 6 (100) | 3 (23) | 16.4 (1.8–471) | 0.008 | 0.016 |
A/A | 0 | 10 (77) | |||
LILRB2_rs7247451 G > C | |||||
n = 6 (%) | n = 13 (%) | ||||
G | 7 (58) | 24 (92) | 0.12 (0.01–0.77) | 0.02 | 0.08 |
C | 5 (42) | 2 (8) | |||
G/G | 2 (33) | 12 (92) | 0.04 (0.0–0.71) 1 | 0.01 | 0.04 |
G/C | 3 (50) | 0 | |||
C/C | 1 (17) | 1 (8) |
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Moraes, A.G.d.; Ayo, C.M.; Elpídio, L.N.S.; Souza, V.H.d.; Yamanaka, A.H.U.; Nogueira, M.L.; Passos, S.D.; Brandão, C.C.; Mattos, L.C.d.; Amaral, G.C.d.; et al. HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil. Curr. Issues Mol. Biol. 2022, 44, 2783-2793. https://doi.org/10.3390/cimb44070191
Moraes AGd, Ayo CM, Elpídio LNS, Souza VHd, Yamanaka AHU, Nogueira ML, Passos SD, Brandão CC, Mattos LCd, Amaral GCd, et al. HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil. Current Issues in Molecular Biology. 2022; 44(7):2783-2793. https://doi.org/10.3390/cimb44070191
Chicago/Turabian StyleMoraes, Amarilis Giaretta de, Christiane Maria Ayo, Laise Nayana Sala Elpídio, Victor Hugo de Souza, Aléia Harumi Uchibaba Yamanaka, Maurício Lacerda Nogueira, Saulo Duarte Passos, Cinara Cássia Brandão, Luiz Carlos de Mattos, Greicy Cezar do Amaral, and et al. 2022. "HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil" Current Issues in Molecular Biology 44, no. 7: 2783-2793. https://doi.org/10.3390/cimb44070191