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Article

A High-Content Screen for the Identification of Plant Extracts with Insulin Secretion-Modulating Activity

1
School of Engineering, University of Applied Sciences Upper Austria, 4600 Wels, Austria
2
FFoQSI—Austrian Competence Center for Feed and Food Quality, 3430 Tulln, Austria
3
PM International AG, 5445 Schengen, Luxembourg
4
Food Chemistry and Molecular Sensory Science, Technical University of Munich, 85354 Freising, Germany
*
Authors to whom correspondence should be addressed.
Academic Editors: Dejan Stojković and Marina Sokovic
Pharmaceuticals 2021, 14(8), 809; https://doi.org/10.3390/ph14080809
Received: 20 May 2021 / Revised: 10 August 2021 / Accepted: 10 August 2021 / Published: 17 August 2021
(This article belongs to the Special Issue Natural Pharmacons: Biologically Active Plant Based Pharmaceuticals)
Bioactive plant compounds and extracts are of special interest for the development of pharmaceuticals. Here, we describe the screening of more than 1100 aqueous plant extracts and synthetic reference compounds for their ability to stimulate or inhibit insulin secretion. To quantify insulin secretion in living MIN6 β cells, an insulin–Gaussia luciferase (Ins-GLuc) biosensor was used. Positive hits included extracts from Quillaja saponaria, Anagallis arvensis, Sapindus mukorossi, Gleditsia sinensis and Albizia julibrissin, which were identified as insulin secretion stimulators, whereas extracts of Acacia catechu, Myrtus communis, Actaea spicata L., Vaccinium vitis-idaea and Calendula officinalis were found to exhibit insulin secretion inhibitory properties. Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were used to characterize several bioactive compounds in the selected plant extracts, and these bioactives were retested for their insulin-modulating properties. Overall, we identified several plant extracts and some of their bioactive compounds that may be used to manipulate pancreatic insulin secretion. View Full-Text
Keywords: insulin; luciferase; natural plant extracts; bioactives; diabetes; β cells; screening; GC-MS; LC-MS; Western blotting; natural compounds insulin; luciferase; natural plant extracts; bioactives; diabetes; β cells; screening; GC-MS; LC-MS; Western blotting; natural compounds
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MDPI and ACS Style

Hager, R.; Pitsch, J.; Kerbl-Knapp, J.; Neuhauser, C.; Ollinger, N.; Iken, M.; Ranner, J.; Mittermeier-Kleßinger, V.; Dawid, C.; Lanzerstorfer, P.; Weghuber, J. A High-Content Screen for the Identification of Plant Extracts with Insulin Secretion-Modulating Activity. Pharmaceuticals 2021, 14, 809. https://doi.org/10.3390/ph14080809

AMA Style

Hager R, Pitsch J, Kerbl-Knapp J, Neuhauser C, Ollinger N, Iken M, Ranner J, Mittermeier-Kleßinger V, Dawid C, Lanzerstorfer P, Weghuber J. A High-Content Screen for the Identification of Plant Extracts with Insulin Secretion-Modulating Activity. Pharmaceuticals. 2021; 14(8):809. https://doi.org/10.3390/ph14080809

Chicago/Turabian Style

Hager, Roland, Johannes Pitsch, Jakob Kerbl-Knapp, Cathrina Neuhauser, Nicole Ollinger, Marcus Iken, Josef Ranner, Verena Mittermeier-Kleßinger, Corinna Dawid, Peter Lanzerstorfer, and Julian Weghuber. 2021. "A High-Content Screen for the Identification of Plant Extracts with Insulin Secretion-Modulating Activity" Pharmaceuticals 14, no. 8: 809. https://doi.org/10.3390/ph14080809

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