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Article

Antiseizure Effects of Fully Characterized Non-Psychoactive Cannabis sativa L. Extracts in the Repeated 6-Hz Corneal Stimulation Test

1
Department of Biomedical, Metabolic, and Neural Sciences, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy
2
PhD School of Clinical and Experimental Medicine (CEM), University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy
3
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
*
Authors to whom correspondence should be addressed.
Academic Editors: Simona Pisanti and Maurizio Bifulco
Pharmaceuticals 2021, 14(12), 1259; https://doi.org/10.3390/ph14121259
Received: 11 November 2021 / Revised: 27 November 2021 / Accepted: 30 November 2021 / Published: 3 December 2021
Compounds present in Cannabis sativa L. preparations have recently attracted much attention in the treatment of drug-resistant epilepsy. Here, we screened two olive oil extracts from a non-psychoactive C. sativa variety, fully characterized by high-performance liquid chromatography and gas chromatography. Particularly, hemp oils with different concentrations of terpenes were administered at the same dose of cannabidiol (25 mg/kg/day orally), 1 h before the 6-Hz corneal stimulation test (44 mA). Mice were stimulated once a day for 5 days and evaluated by video-electrocorticographic recordings and behavioral analysis. Neuronal activation was assessed by FosB/ΔFosB immunoreactivity. Both oils significantly reduced the percentage of mice experiencing convulsive seizures in comparison to olive oil-treated mice (p < 0.050; Fisher’s exact test), but only the oil enriched with terpenes (K2) significantly accelerated full recovery from the seizure. These effects occurred in the presence of reduced power of delta rhythm, and, instead, increased power of theta rhythm, along with a lower FosB/ΔFosB expression in the subiculum (p < 0.050; Duncan’s method). The overall findings suggest that both cannabinoids and terpenes in oil extracts should be considered as potential therapeutic agents against epileptic seizures and epilepsy. View Full-Text
Keywords: Cannabis sativa L.; hemp; cannabidiol; terpenes; epilepsy; 6-Hz corneal stimulation; antiseizure activity; power band spectrum; FosB/ΔFosB Cannabis sativa L.; hemp; cannabidiol; terpenes; epilepsy; 6-Hz corneal stimulation; antiseizure activity; power band spectrum; FosB/ΔFosB
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MDPI and ACS Style

Costa, A.-M.; Senn, L.; Anceschi, L.; Brighenti, V.; Pellati, F.; Biagini, G. Antiseizure Effects of Fully Characterized Non-Psychoactive Cannabis sativa L. Extracts in the Repeated 6-Hz Corneal Stimulation Test. Pharmaceuticals 2021, 14, 1259. https://doi.org/10.3390/ph14121259

AMA Style

Costa A-M, Senn L, Anceschi L, Brighenti V, Pellati F, Biagini G. Antiseizure Effects of Fully Characterized Non-Psychoactive Cannabis sativa L. Extracts in the Repeated 6-Hz Corneal Stimulation Test. Pharmaceuticals. 2021; 14(12):1259. https://doi.org/10.3390/ph14121259

Chicago/Turabian Style

Costa, Anna-Maria, Lara Senn, Lisa Anceschi, Virginia Brighenti, Federica Pellati, and Giuseppe Biagini. 2021. "Antiseizure Effects of Fully Characterized Non-Psychoactive Cannabis sativa L. Extracts in the Repeated 6-Hz Corneal Stimulation Test" Pharmaceuticals 14, no. 12: 1259. https://doi.org/10.3390/ph14121259

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