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Article

Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil

1
Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, Italy
2
High Equipment Centre, Tuscia University, 01100 Viterbo, Italy
3
Department of Drug Chemistry and Technology, Sapienza University, 00185 Roma RM, Italy
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2020, 13(11), 352; https://doi.org/10.3390/ph13110352
Received: 23 September 2020 / Revised: 19 October 2020 / Accepted: 26 October 2020 / Published: 29 October 2020
Lavandin essential oil (LEO), a natural sterile hybrid obtained by crossbreeding L. angustifolia × L. latifolia, is mainly composed by active components belonging to the family of terpenes endowed with relevant anti-proliferative activity, which can be enhanced by proper application of nanotechnology. In particular, this study reports the chemical characterization and the screening of the anti-proliferative activity on different human cell lines of pure and nano-formulated lavandin essential oil (EO). LEO and its formulation (NanoLEO) were analyzed by HS/GC-MS (Headspace/Gas Chromatography-Mass Spectrometry) to describe and compare their chemical volatile composition. The most abundant compounds were linalool and 1,8-cineole (LEO: 28.6%; 27.4%) (NanoLEO: 60.4%; 12.6%) followed by α-pinene (LEO: 9.6%; NanoLEO: 4.5%), camphor (LEO: 6.5%; NanoLEO: 7.0%) and linalyl acetate (LEO: 6.5%; NanoLEO: 3.6%). The cytotoxic effects of LEO and NanoLEO were investigated on human neuroblastoma cells (SHSY5Y), human breast adenocarcinoma cells (MCF-7), human lymphoblastic leukemia cells (CCRF CEM), human colorectal adenocarcinoma cells (Caco-2) and one normal breast epithelial cell (MCF10A) by the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)-assay. Caco-2, MCF7 and MCF10A normal cells resulted more resistant to the treatment with LEO, while CCRF-CEM and SHSY5Y cells were more sensitive. The antiproliferative effect of LEO resulted amplified when the essential oil was supplied as nanoformulation, mainly in Caco-2 cells. Scanning and transmission electron microscopy investigations were carried out on Caco-2 cells to outline at ultrastructural level possible affections induced by LEO and NanoLEO treatments. View Full-Text
Keywords: lavandin essential oil; antiproliferative activity; HS-GC/MS analysis; nanoemulsion lavandin essential oil; antiproliferative activity; HS-GC/MS analysis; nanoemulsion
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MDPI and ACS Style

Ovidi, E.; Masci, V.L.; Taddei, A.R.; Paolicelli, P.; Petralito, S.; Trilli, J.; Mastrogiovanni, F.; Tiezzi, A.; Casadei, M.A.; Giacomello, P.; Garzoli, S. Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil. Pharmaceuticals 2020, 13, 352. https://doi.org/10.3390/ph13110352

AMA Style

Ovidi E, Masci VL, Taddei AR, Paolicelli P, Petralito S, Trilli J, Mastrogiovanni F, Tiezzi A, Casadei MA, Giacomello P, Garzoli S. Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil. Pharmaceuticals. 2020; 13(11):352. https://doi.org/10.3390/ph13110352

Chicago/Turabian Style

Ovidi, Elisa, Valentina L. Masci, Anna R. Taddei, Patrizia Paolicelli, Stefania Petralito, Jordan Trilli, Fabio Mastrogiovanni, Antonio Tiezzi, Maria A. Casadei, Pierluigi Giacomello, and Stefania Garzoli. 2020. "Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil" Pharmaceuticals 13, no. 11: 352. https://doi.org/10.3390/ph13110352

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