Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation
Abstract
:1. Introduction
2. Results
2.1. Construction of Transposon Vectors to Express Cerulean, Mcherry, and Mcitrine in Neurons, Astrocytes, and Oligodendrocytes
2.2. Generation of Triple-Tg Zebrafish
2.3. Assessment of DNT Using Triple-Tg Zebrafish
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Compounds
4.3. Zebrafish Husbandry
4.4. Generation of Tg (eno2:Cerulean, gfap:mCherry, mbp:mCitrine) Zebrafish
4.5. Exposure of Triple-Tg Zebrafish to Chemicals
4.6. In Vivo Imaging of Triple-Tg Zebrafish
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Koiwa, J.; Shiromizu, T.; Adachi, Y.; Ikejiri, M.; Nakatani, K.; Tanaka, T.; Nishimura, Y. Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation. Pharmaceuticals 2019, 12, 145. https://doi.org/10.3390/ph12040145
Koiwa J, Shiromizu T, Adachi Y, Ikejiri M, Nakatani K, Tanaka T, Nishimura Y. Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation. Pharmaceuticals. 2019; 12(4):145. https://doi.org/10.3390/ph12040145
Chicago/Turabian StyleKoiwa, Junko, Takashi Shiromizu, Yuka Adachi, Makoto Ikejiri, Kaname Nakatani, Toshio Tanaka, and Yuhei Nishimura. 2019. "Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation" Pharmaceuticals 12, no. 4: 145. https://doi.org/10.3390/ph12040145