Next Article in Journal
A π-Halogen Bond of Dibenzofuranones with the Gatekeeper Phe113 in Human Protein Kinase CK2 Leads to Potent Tight Binding Inhibitors
Next Article in Special Issue
Mitoxantrone is More Toxic than Doxorubicin in SH-SY5Y Human Cells: A ‘Chemobrain’ In Vitro Study
Previous Article in Journal
Pilocarpine-Induced Status Epilepticus Is Associated with P-Glycoprotein Induction in Cardiomyocytes, Electrocardiographic Changes, and Sudden Death
Previous Article in Special Issue
Drug Repurposing for Schistosomiasis: Combinations of Drugs or Biomolecules
Open AccessArticle

A Novel Interaction Between the TLR7 and a Colchicine Derivative Revealed Through a Computational and Experimental Study

Department of Physics, University of Alberta, AB T6G 2E1 Edmonton, Canada
Istituto Dalle Molle di Studi Sull’intelligenza Artificiale (IDSIA), Scuola Universitaria Professionale della Svizzera Italiana (SUPSI), Università della Svizzera Italiana (USI), CH-6928 Manno, Switzerland
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, AB T6G 2H1 Edmonton, Canada
Department of Mechanical and Aerospace Engineering, Politecnico di Torino, 10129 Torino, Italy
Department of Oncology, University of Alberta, AB T6G 1Z2 Edmonton, Canada
Author to whom correspondence should be addressed.
Pharmaceuticals 2018, 11(1), 22;
Received: 17 January 2018 / Revised: 1 February 2018 / Accepted: 13 February 2018 / Published: 16 February 2018
(This article belongs to the Special Issue Old Pharmaceuticals with New Applications)
The Toll-Like Receptor 7 (TLR7) is an endosomal membrane receptor involved in the innate immune system response. Its best-known small molecule activators are imidazoquinoline derivatives such as imiquimod (R-837) and resiquimod (R-848). Recently, an interaction between R-837 and the colchicine binding site of tubulin was reported. To investigate the possibility of an interaction between structural analogues of colchicine and the TLR7, a recent computational model for the dimeric form of the TLR7 receptor was used to determine a possible interaction with a colchicine derivative called CR42-24, active as a tubulin polymerization inhibitor. The estimated values of the binding energy of this molecule with respect to the TLR7 receptor were comparable to the energies of known binders as reported in a previous study. The binding to the TLR7 was further assessed by introducing genetic transformations in the TLR7 gene in cancer cell lines and exposing them to the compound. A negative shift of the IC50 value in terms of cell growth was observed in cell lines carrying the mutated TLR7 gene. The reported study suggests a possible interaction between TLR7 and a colchicine derivative, which can be explored for rational design of new drugs acting on this receptor by using a colchicine scaffold for additional modifications. View Full-Text
Keywords: TLR7; colchicine; imiquimod; innate immune system; off-target interaction TLR7; colchicine; imiquimod; innate immune system; off-target interaction
Show Figures

Figure 1

MDPI and ACS Style

Gentile, F.; Deriu, M.A.; Barakat, K.; Danani, A.; Tuszynski, J. A Novel Interaction Between the TLR7 and a Colchicine Derivative Revealed Through a Computational and Experimental Study. Pharmaceuticals 2018, 11, 22.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop