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Sensors 2009, 9(6), 4164-4177;

Fluorescence Detection and Discrimination of ss- and ds-DNA with a Water Soluble Oligopyrene Derivative

Key Lab of Bio-organic Phosphorous Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China
Author to whom correspondence should be addressed.
Received: 9 April 2009 / Revised: 1 May 2009 / Accepted: 21 May 2009 / Published: 2 June 2009
(This article belongs to the Section Biosensors)
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A novel water-soluble cationic conjugated oligopyrene derivative, oligo(N1,N1,N1,N4,N4,N4-hexamethyl-2-(4-(pyren-1-yl) butane-1,4-diaminium bromide) (OHPBDB), was synthesized by a combination of chemical and electrochemical synthesis techniques. Each oligomer chain has five pyrene derivative repeating units and brings 10 positive charges. OHPBDB showed high and rapid fluorescence quenching in aqueous media upon addition of trace amounts of single-stranded (ss) and double-stranded (ds) DNA. The Stern-Volmer constants for ss- and ds-DNA were measured to be as high as 1.3 × 108 mol-1·L and 1.2 × 108 mol-1·L, respectively. On the other hand, distinct fluorescence enhancement of OHPBDB upon addition of large amount of ss-DNA or ds-DNA was observed. Furthermore, ss-DNA showed much stronger fluorescence enhancement than that of ds-DNA, thus yielding a clear and simple signal useful for the discrimination between ss- and ds-DNA in aqueous media. View Full-Text
Keywords: water-soluble oligopyrene derivative; DNA; fluorescence quenching water-soluble oligopyrene derivative; DNA; fluorescence quenching
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Chen, Y.; Shi, G. Fluorescence Detection and Discrimination of ss- and ds-DNA with a Water Soluble Oligopyrene Derivative. Sensors 2009, 9, 4164-4177.

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