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Open AccessArticle

Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure

1
Group UVASENS, Escuela de Ingenierías Industriales, Universidad de Valladolid, Paseo del Cauce, 59, 47011 Valladolid, Spain
2
Bioeco UVA Research Institute, Universidad de Valladolid, 47011 Valladolid, Spain
3
Dpt. of Materials Science, Universidad de Valladolid, Paseo del Cauce, 59, 47011 Valladolid, Spain
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(7), 2152; https://doi.org/10.3390/s20072152
Received: 16 March 2020 / Revised: 6 April 2020 / Accepted: 9 April 2020 / Published: 10 April 2020
(This article belongs to the Section Biosensors)
Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances. View Full-Text
Keywords: catechol; gold nanoparticles; sulfonated phthalocyanine; laccase; tyrosinase; layer-by-layer catechol; gold nanoparticles; sulfonated phthalocyanine; laccase; tyrosinase; layer-by-layer
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MDPI and ACS Style

Salvo-Comino, C.; González-Gil, A.; Rodriguez-Valentin, J.; Garcia-Hernandez, C.; Martin-Pedrosa, F.; Garcia-Cabezon, C.; Rodriguez-Mendez, M.L. Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure. Sensors 2020, 20, 2152.

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