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Toward Sub-Diffraction Imaging of Single-DNA Molecule Sensors Based on Stochastic Switching Localization Microscopy

Department of Applied Chemistry and Institute of Natural Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2020, 20(22), 6667; https://doi.org/10.3390/s20226667
Received: 15 October 2020 / Revised: 14 November 2020 / Accepted: 20 November 2020 / Published: 21 November 2020
(This article belongs to the Special Issue DNA-Based Sensors for Single-Molecule Biology)
The natural characteristics of deoxyribonucleic acid (DNA) enable its advanced applications in nanotechnology as a special tool that can be detected by high-resolution imaging with precise localization. Super-resolution (SR) microscopy enables the examination of nanoscale molecules beyond the diffraction limit. With the development of SR microscopy methods, DNA nanostructures can now be optically assessed. Using the specific binding of fluorophores with their target molecules, advanced single-molecule localization microscopy (SMLM) has been expanded into different fields, allowing wide-range detection at the single-molecule level. This review discusses the recent progress in the SR imaging of DNA nano-objects using SMLM techniques, such as direct stochastic optical reconstruction microscopy, binding-activated localization microscopy, and point accumulation for imaging nanoscale topography. Furthermore, we discuss their advantages and limitations, present applications, and future perspectives. View Full-Text
Keywords: single-DNA molecule sensor; DNA nanoarchitectures; direct stochastic optical reconstruction microscopy; sub-diffraction imaging; super-resolution microscopy single-DNA molecule sensor; DNA nanoarchitectures; direct stochastic optical reconstruction microscopy; sub-diffraction imaging; super-resolution microscopy
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MDPI and ACS Style

Lee, S.; Batjikh, I.; Kang, S.H. Toward Sub-Diffraction Imaging of Single-DNA Molecule Sensors Based on Stochastic Switching Localization Microscopy. Sensors 2020, 20, 6667.

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