Aptamer-Conjugated Polydiacetylene Colorimetric Paper Chip for the Detection of Bacillus thuringiensis Spores

Zhou, Chaoge; You, Taeyeong; Jang, Huisoo; Ryu, Hyunil; Lee, Eun-Seon; Oh, Mi-Hwa; Huh, Yun Suk; Kim, Sun Min; Jeon, Tae-Joon

doi:10.3390/s20113124

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Aptamer-Conjugated Polydiacetylene Colorimetric Paper Chip for the Detection of Bacillus thuringiensis Spores

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Tae-Joon Jeon

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Department of Biological Engineering and Biohybrid Systems Research Center (BSRC), Inha University, 100 Inha-ro, Michuhol-gu, Incheon 22212, Korea

National Institute of Animal Science, Rural Development Administration, Jeollabuk-do 55365, Korea

Department of Mechanical Engineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon 22212, Korea

Authors to whom correspondence should be addressed.

Sensors 2020, 20(11), 3124; https://doi.org/10.3390/s20113124

Received: 14 April 2020 / Revised: 27 May 2020 / Accepted: 29 May 2020 / Published: 1 June 2020

(This article belongs to the Special Issue DNA-Based Sensors for Single-Molecule Biology)

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Abstract

A colorimetric polydiacetylene (PDA) paper strip sensor that can specifically recognize Bacillus thuringiensis (BT) HD-73 spores is described in this work. The target-specific aptamer was combined with PDA, and the aptamer-conjugated PDA vesicles were then coated on polyvinylidene fluoride (PVDF) paper strips by a simple solvent evaporation method. The PDA-aptamer paper strips can be used to detect the target without any pre-treatment. Using the paper strip, the presence of BT spores is directly observable by the naked eye based on the unique blue-to-red color transition of the PDA. Quantitative studies using the paper strip were also carried out by analyzing the color transitions of the PDA. The specificity of this PDA sensor was verified with a high concentration of Escherichia coli, and no discernable change was observed. The observable color change in the paper strip occurs in less than 1 h, and the limit of detection is 3 × 10⁷ CFU/mL, much below the level harmful to humans. The PDA-based paper sensor, developed in this work, does not require a separate power or detection device, making the sensor strip highly transportable and suitable for spore analysis anytime and anywhere. Moreover, this paper sensor platform is easily fabricated, can be adapted to other targets, is highly portable, and is highly specific for the detection of BT spores. View Full-Text

Keywords: polydiacetylene (PDA); Bacillus thuringiensis spores; aptamer; paper chip; chromatic sensor

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MDPI and ACS Style

Zhou, C.; You, T.; Jang, H.; Ryu, H.; Lee, E.-S.; Oh, M.-H.; Huh, Y.S.; Kim, S.M.; Jeon, T.-J. Aptamer-Conjugated Polydiacetylene Colorimetric Paper Chip for the Detection of Bacillus thuringiensis Spores. Sensors 2020, 20, 3124. https://doi.org/10.3390/s20113124

AMA Style

Zhou C, You T, Jang H, Ryu H, Lee E-S, Oh M-H, Huh YS, Kim SM, Jeon T-J. Aptamer-Conjugated Polydiacetylene Colorimetric Paper Chip for the Detection of Bacillus thuringiensis Spores. Sensors. 2020; 20(11):3124. https://doi.org/10.3390/s20113124

Chicago/Turabian Style

Zhou, Chaoge, Taeyeong You, Huisoo Jang, Hyunil Ryu, Eun-Seon Lee, Mi-Hwa Oh, Yun S. Huh, Sun M. Kim, and Tae-Joon Jeon. 2020. "Aptamer-Conjugated Polydiacetylene Colorimetric Paper Chip for the Detection of Bacillus thuringiensis Spores" Sensors 20, no. 11: 3124. https://doi.org/10.3390/s20113124

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Aptamer-Conjugated Polydiacetylene Colorimetric Paper Chip for the Detection of Bacillus thuringiensis Spores

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