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Sensors 2018, 18(4), 990; https://doi.org/10.3390/s18040990

Electronic Detection of DNA Hybridization by Coupling Organic Field-Effect Transistor-Based Sensors and Hairpin-Shaped Probes

1
Department of Electrical and Electronic Engineering, Università di Cagliari, 09123 Cagliari, Italy
2
Istituto di Fisica Applicata “Nello Carrara”, Consiglio Nazionale delle Ricerche, 50019 Sesto Fiorentino, Italy
*
Author to whom correspondence should be addressed.
Received: 23 February 2018 / Revised: 20 March 2018 / Accepted: 23 March 2018 / Published: 27 March 2018
(This article belongs to the Special Issue Label-Free Biosensors)
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Abstract

In this paper, the electronic transduction of DNA hybridization is presented by coupling organic charge-modulated field-effect transistors (OCMFETs) and hairpin-shaped probes. These probes have shown interesting properties in terms of sensitivity and selectivity in other kinds of assays, in the form of molecular beacons (MBs). Their integration with organic-transistor based sensors, never explored before, paves the way to a new class of low-cost, easy-to-use, and portable genetic sensors with enhanced performances. Thanks to the peculiar characteristics of the employed sensor, measurements can be performed at relatively high ionic strengths, thus optimizing the probes’ functionality without affecting the detection ability of the device. A complete electrical characterization of the sensor is reported, including calibration with different target concentrations in the measurement environment and selectivity evaluation. In particular, DNA hybridization detection for target concentration as low as 100 pM is demonstrated. View Full-Text
Keywords: organic field-effect transistors; DNA hybridization detection; hairpin-shaped oligonucleotides; electronic sensors organic field-effect transistors; DNA hybridization detection; hairpin-shaped oligonucleotides; electronic sensors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Napoli, C.; Lai, S.; Giannetti, A.; Tombelli, S.; Baldini, F.; Barbaro, M.; Bonfiglio, A. Electronic Detection of DNA Hybridization by Coupling Organic Field-Effect Transistor-Based Sensors and Hairpin-Shaped Probes. Sensors 2018, 18, 990.

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