Next Article in Journal
A Sensor Fusion Method Based on an Integrated Neural Network and Kalman Filter for Vehicle Roll Angle Estimation
Next Article in Special Issue
Highly Selective and Ultrasensitive Turn-on Luminescence Chemosensor for Mercury (II) Determination Based on the Rhodamine 6G Derivative FC1 and Au Nanoparticles
Previous Article in Journal
Resonance Frequency of Optical Microbubble Resonators: Direct Measurements and Mitigation of Fluctuations
Previous Article in Special Issue
Upconversion Nanoparticle-Based Förster Resonance Energy Transfer for Detecting DNA Methylation
Open AccessArticle

A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells

1
Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore
2
Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
*
Author to whom correspondence should be addressed.
Academic Editors: Jong Seung Kim and Min Hee Lee
Sensors 2016, 16(9), 1397; https://doi.org/10.3390/s16091397
Received: 2 August 2016 / Revised: 23 August 2016 / Accepted: 26 August 2016 / Published: 31 August 2016
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensor)
Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM). View Full-Text
Keywords: BODIPY; fluorescent probes; sensors; viscosity; FLIM; cell imaging BODIPY; fluorescent probes; sensors; viscosity; FLIM; cell imaging
Show Figures

Figure 1

MDPI and ACS Style

Su, D.; Teoh, C.L.; Gao, N.; Xu, Q.-H.; Chang, Y.-T. A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells. Sensors 2016, 16, 1397.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop