Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.1.1. Quantum Dots
2.1.2. Tb Complex
2.1.3. Antigens and Antibodies
2.1.4. Chemicals and Biochemicals
2.2. Methods
2.2.1. Tb-Antibody (Tb-AB) Conjugation
2.2.2. QD-Antibody (QD-AB) Conjugation
2.2.3. Photophysical Characterization
2.2.4. FRET Characterization
2.2.5. FRET Immunoassays
3. Results and Discussion
3.1. Tb and QD AB Conjugates and Tb-QD FRET-Pairs
3.2. Homogeneous Time-Resolved FRET Immunoassay
Donor-AB | Tb/AB | Acceptor-AB | AB/QD | R0 (nm) | LOD (nM) | LOD (ng/mL) |
---|---|---|---|---|---|---|
Tb-IgG | 6 ± 1 | QD605-F(ab) | 5 ± 2 | 8.8 ± 0.4 | 0.71 ± 0.07 | 23 ± 2 |
QD655-F(ab) | 8 ± 3 | 10.5 ± 0.5 | 0.12 ± 0.01 | 3.7 ± 0.4 | ||
QD705-F(ab) | 6 ± 2 | 11.2 ± 0.6 | 0.06 ± 0.01 | 2.0 ± 0.3 |
3.3. Duplexed Tb-to-QD FRET Immunoassay
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Bhuckory, S.; Lefebvre, O.; Qiu, X.; Wegner, K.D.; Hildebrandt, N. Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen. Sensors 2016, 16, 197. https://doi.org/10.3390/s16020197
Bhuckory S, Lefebvre O, Qiu X, Wegner KD, Hildebrandt N. Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen. Sensors. 2016; 16(2):197. https://doi.org/10.3390/s16020197
Chicago/Turabian StyleBhuckory, Shashi, Olivier Lefebvre, Xue Qiu, Karl David Wegner, and Niko Hildebrandt. 2016. "Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen" Sensors 16, no. 2: 197. https://doi.org/10.3390/s16020197