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Sensors 2015, 15(5), 10059-10073;

Sensitive, Fast, and Specific Immunoassays for Methyltestosterone Detection

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 27 January 2015 / Revised: 21 March 2015 / Accepted: 27 April 2015 / Published: 29 April 2015
(This article belongs to the Special Issue Immunosensors 2014)
Full-Text   |   PDF [1372 KB, uploaded 29 April 2015]   |  


An indirect competitive enzyme-linked immunosorbent assay (icELISA) and an immunochromatographic strip assay using a highly specific monoclonal antibody, were developed to detect methyltestosterone (MT) residues in animal feed. The optimized icELISA had a half-inhibition concentration value of 0.26 ng/mL and a limit of detection value of 0.045 ng/mL. There was no cross-reactivity with eight analogues, revealing high specificity for MT. Based on icELISA results, the recovery rate of MT in animal feed was 82.4%–100.6%. The results were in accordance with those obtained by gas chromatography-mass spectrometry. The developed immunochromatographic strip assay, as the first report for MT detection, had a visual cut-off value of 1 ng/mL in PBS, 2.5 ng/g in fish feed, and 2.5 ng/g in pig feed. Therefore, these immunoassays are useful and fast tools for MT residue detection in animal feed. View Full-Text
Keywords: icELISA; immunochromatographic strip assay; monoclonal antibody; methyltestosterone; fast detection icELISA; immunochromatographic strip assay; monoclonal antibody; methyltestosterone; fast detection

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kong, N.; Song, S.; Peng, J.; Liu, L.; Kuang, H.; Xu, C. Sensitive, Fast, and Specific Immunoassays for Methyltestosterone Detection. Sensors 2015, 15, 10059-10073.

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