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Open AccessArticle

Sensitive, Fast, and Specific Immunoassays for Methyltestosterone Detection

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
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Academic Editor: Alexander Star
Sensors 2015, 15(5), 10059-10073; https://doi.org/10.3390/s150510059
Received: 27 January 2015 / Revised: 21 March 2015 / Accepted: 27 April 2015 / Published: 29 April 2015
(This article belongs to the Special Issue Immunosensors 2014)
An indirect competitive enzyme-linked immunosorbent assay (icELISA) and an immunochromatographic strip assay using a highly specific monoclonal antibody, were developed to detect methyltestosterone (MT) residues in animal feed. The optimized icELISA had a half-inhibition concentration value of 0.26 ng/mL and a limit of detection value of 0.045 ng/mL. There was no cross-reactivity with eight analogues, revealing high specificity for MT. Based on icELISA results, the recovery rate of MT in animal feed was 82.4%–100.6%. The results were in accordance with those obtained by gas chromatography-mass spectrometry. The developed immunochromatographic strip assay, as the first report for MT detection, had a visual cut-off value of 1 ng/mL in PBS, 2.5 ng/g in fish feed, and 2.5 ng/g in pig feed. Therefore, these immunoassays are useful and fast tools for MT residue detection in animal feed. View Full-Text
Keywords: icELISA; immunochromatographic strip assay; monoclonal antibody; methyltestosterone; fast detection icELISA; immunochromatographic strip assay; monoclonal antibody; methyltestosterone; fast detection
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MDPI and ACS Style

Kong, N.; Song, S.; Peng, J.; Liu, L.; Kuang, H.; Xu, C. Sensitive, Fast, and Specific Immunoassays for Methyltestosterone Detection. Sensors 2015, 15, 10059-10073.

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