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A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes

Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico
Coordinación Académica, Universidad Autónoma de la Ciudad de México, México D.F., 09620, Mexico
Centro de Investigación en Micro y Nanotecnología (MICRONA), Universidad Veracruzana, Boca del Rio, Veracruz, 94294, Mexico
Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT), Texcoco, Estado de Mexico, 56237, Mexico
Department of Physics, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico
Laboratorio Estatal de Salud Pública de Oaxaca, Servicios de Salud de Oaxaca, Oaxaca, 71257, Mexico
Escuela de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juarez de Oaxaca, 68120, Oaxaca, Mexico
División de Epidemiologia, Coordinación de Programas Integrados de Salud, Instituto Mexicano del Seguro Social, Mexico D.F., 06700, Mexico
Author to whom correspondence should be addressed.
Sensors 2014, 14(5), 7580-7601;
Received: 17 January 2014 / Revised: 10 March 2014 / Accepted: 26 March 2014 / Published: 25 April 2014
(This article belongs to the Special Issue Microarray Sensors)
PDF [770 KB, uploaded 21 June 2014]


Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples. View Full-Text
Keywords: dengue virus; humans; Aedes; microarrays dengue virus; humans; Aedes; microarrays

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Díaz-Badillo, A.; De Lourdes Muñoz, M.; Perez-Ramirez, G.; Altuzar, V.; Burgueño, J.; Mendoza-Alvarez, J.G.; Martínez-Muñoz, J.P.; Cisneros, A.; Navarrete-Espinosa, J.; Sanchez-Sinencio, F. A DNA Microarray-Based Assay to Detect Dual Infection with Two Dengue Virus Serotypes. Sensors 2014, 14, 7580-7601.

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