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Screening of Aptamers on Microfluidic Systems for Clinical Applications

Department of Microbiology and Immunology, National Cheng Kung University, Tainan 70101, Taiwan
Department of Mechanical Engineering, National Taiwan University, Taipei 10617, Taiwan
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan
Author to whom correspondence should be addressed.
Sensors 2012, 12(7), 9514-9529;
Received: 30 May 2012 / Revised: 2 July 2012 / Accepted: 6 July 2012 / Published: 11 July 2012
(This article belongs to the Special Issue Micro and Nano Technologies for Point-of-Care Diagnosis)
PDF [501 KB, uploaded 21 June 2014]


The use of microfluidic systems for screening of aptamers and their biomedical applications are reviewed in this paper. Aptamers with different nucleic acid sequences have been extensively studied and the results demonstrated a strong binding affinity to target molecules such that they can be used as promising candidate biomarkers for diagnosis and therapeutics. Recently, the aptamer screening protocol has been conducted with microfluidic-based devices. Furthermore, aptamer affinity screening by a microfluidic-based method has demonstrated remarkable advantages over competing traditional methods. In this paper, we first reviewed microfluidic systems which demonstrated efficient and rapid screening of a specific aptamer. Then, the clinical applications of screened aptamers, also performed by microfluidic systems, are further reviewed. These automated microfluidic systems can provide advantages over their conventional counterparts including more compactness, faster analysis, less sample/reagent consumption and automation. An aptamer-based compact microfluidic system for diagnosis may even lead to a point-of-care device. The use of microfluidic systems for aptamer screening and diagnosis is expected to continue growing in the near future and may make a substantial impact on biomedical applications. View Full-Text
Keywords: microfluidics; SELEX; aptamer; biosensor; MEMS microfluidics; SELEX; aptamer; biosensor; MEMS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Weng, C.-H.; Huang, C.-J.; Lee, G.-B. Screening of Aptamers on Microfluidic Systems for Clinical Applications. Sensors 2012, 12, 9514-9529.

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