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Open AccessArticle

N-Acyl Homoserine Lactone Production by Klebsiella pneumoniae Isolated from Human Tongue Surface

1
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
2
School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
3
Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, 637616, Singapore
*
Author to whom correspondence should be addressed.
Sensors 2012, 12(3), 3472-3483; https://doi.org/10.3390/s120303472
Received: 12 January 2012 / Revised: 16 February 2012 / Accepted: 23 February 2012 / Published: 12 March 2012
(This article belongs to the Section Biosensors)
Bacteria communicate by producing quorum sensing molecules called autoinducers, which include autoinducer-1, an N-hexanoyl homoserine lactone (AHL), and autoinducer-2. Bacteria present in the human oral cavity have been shown to produce autoinducer-2, but not AHL. Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual. Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs. High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone. To the best of our knowledge, this is the first report of the isolation of K. pneumoniae from the posterior dorsal surface of the human tongue and the production of these AHLs by this bacterium. View Full-Text
Keywords: N-acylhomoserine lactone; N-3-dodecanoyl-L-homoserine lactone (C12-HSL); N-heptanoyl-L-homoserine lactone (C7-HSL); N-octanoyl-L-homoserine lactone (C8-HSL); oral cavity; posterior dorsal surface; quorum sensing; tongue N-acylhomoserine lactone; N-3-dodecanoyl-L-homoserine lactone (C12-HSL); N-heptanoyl-L-homoserine lactone (C7-HSL); N-octanoyl-L-homoserine lactone (C8-HSL); oral cavity; posterior dorsal surface; quorum sensing; tongue
MDPI and ACS Style

Yin, W.-F.; Purmal, K.; Chin, S.; Chan, X.-Y.; Koh, C.-L.; Sam, C.-K.; Chan, K.-G. N-Acyl Homoserine Lactone Production by Klebsiella pneumoniae Isolated from Human Tongue Surface. Sensors 2012, 12, 3472-3483.

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