Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit
by
Gonzalo García-Martinez 1, Enrique Alonso Bustabad 1, Hubert Perrot 2,3,*, Claude Gabrielli 2, Bogdan Bucur 2,3, Mathieu Lazerges 2,3, Daniel Rose 2,3, Loreto Rodriguez-Pardo 1, Jose Fariña 1, Chantal Compère 4 and Antonio Arnau Vives 5
Gonzalo García-Martinez 1, Enrique Alonso Bustabad 1, Hubert Perrot 2,3,*, Claude Gabrielli 2, Bogdan Bucur 2,3, Mathieu Lazerges 2,3, Daniel Rose 2,3, Loreto Rodriguez-Pardo 1, Jose Fariña 1, Chantal Compère 4 and Antonio Arnau Vives 5
1
Dpto. Tecnología Electrónica, Universidad de Vigo, Campus Lagoas Marcosende, Vigo 36310, Spain
2
LISE (UPR15), CNRS/4, Place Jussieu, cp 133, Paris cedex 05 75252, France
3
Université P. et M. Curie/4, Place Jussieu, cp 133, Paris cedex 05 75252, France
4
IFREMER, Service Interfaces et Capteurs, Centre de Brest, BP 70, Plouzané 29280, France
5
Wave Phenomena Group, Electronic Engineering Department, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain
*
Author to whom correspondence should be addressed.
Sensors 2011, 11(8), 7656-7664; https://doi.org/10.3390/s110807656
Received: 8 July 2011 / Revised: 31 July 2011 / Accepted: 1 August 2011 / Published: 3 August 2011
(This article belongs to the Section Biosensors)
This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm2 in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
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Keywords:
DNA sequence detection; quartz crystal microbalance; electronic oscillator; sensitivity; frequency noise; resolution
This is an open access article distributed under the Creative Commons Attribution License
MDPI and ACS Style
García-Martinez, G.; Bustabad, E.A.; Perrot, H.; Gabrielli, C.; Bucur, B.; Lazerges, M.; Rose, D.; Rodriguez-Pardo, L.; Fariña, J.; Compère, C.; Vives, A.A. Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit. Sensors 2011, 11, 7656-7664.
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