Elmis syriaca (Kuwert, 1890) and E. zoufali (Reitter, 1910) (Coleoptera: Elmidae) confirmed as distinct species based on molecular data, morphology and geographical distribution
Abstract
:1. Introduction
2. Material and Methods
2.1. Morphological Examination of Specimens
2.2. DNA Extraction, Amplification and Sequencing
2.3. Sequence Data Analyses
3. Results
3.1. Morphology and Distribution
3.2. Identification, Delimitation and Divergence Time Calibration Based on Molecular Data
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Country | BIN | BOLD Process ID/GenBank acc. nr. for COI 1,2 | ITS1 | H3 | CAD |
---|---|---|---|---|---|---|
Elmis zoufali | Croatia Serbia Serbia Serbia Albania Bulgaria Bulgaria | BOLD:ADP5230 | ELMEU105-18/- ELMEU101-18/- CROEL003-20/OR138318 CROEL019-20/OR138316 NMWEL003-23/OR138311 NMWEL004-23/OR138310 ELMEU103-18/- | - - OR142274 OR142273 OR142271 OR142272 - | - - OR146742 OR146741 OR146739 OR146740 - | - - OR146727 OR146726 OR146724 OR146725 - |
Elmis syriaca | Israel Israel Israel Israel Israel Israel Georgia Georgia Georgia Armenia Azerbaijan Azerbaijan | BOLD:AFC9293 | -/OR187603 -/OR187604 -/OR187605 -/OR187606 NMWEL007-23/OR138313 NMWEL008-23/OR138308 NMWEL005-23/OR138305 NMWEL006-23/OR138309 NMWEL010-23/OR138306 NMWEL013-23/OR138312 NMWEL009-23/OR138307 NMWEL011-23/OR138315 | - - - - OR142269 OR142270 - OR142267 OR142268 - OR142266 OR142265 | - - - - OR146737 OR146738 OR146734 OR146735 OR146736 OR146733 OR146732 OR146731 | - - - - OR146722 OR146723 OR146719 OR146720 OR146721 OR146718 OR146717 OR146716 |
Elmis ? quadricollis | China China | BOLD:ADL0116 | NMWEL012-23/OR138314 XJDQD253-18/- | OR142275 - | - - | - - |
Elmis aenea | Croatia | BOLD:AAF0074 | CROBF005-19/OR138317 | OR142277 | OR146743 | OR146729 |
Elmis rioloides | Croatia | BOLD:ABY6700 | CROBF017-19/OR138319 | OR142278 | OR146744 | OR146730 |
Elmis bosnica | Croatia | BOLD:ADR7537 | CROBF008-19/OL874462 | OR142276 | - | OR146728 |
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Jäch, M.A.; Brojer, M.; Mičetić Stanković, V.; Bošnjak, M.; Luz, D.; Dorchin, N.; Hershkovitz, Y.; Novaković, B.; Živić, I.; Dorfer, W.; et al. Elmis syriaca (Kuwert, 1890) and E. zoufali (Reitter, 1910) (Coleoptera: Elmidae) confirmed as distinct species based on molecular data, morphology and geographical distribution. Diversity 2023, 15, 994. https://doi.org/10.3390/d15090994
Jäch MA, Brojer M, Mičetić Stanković V, Bošnjak M, Luz D, Dorchin N, Hershkovitz Y, Novaković B, Živić I, Dorfer W, et al. Elmis syriaca (Kuwert, 1890) and E. zoufali (Reitter, 1910) (Coleoptera: Elmidae) confirmed as distinct species based on molecular data, morphology and geographical distribution. Diversity. 2023; 15(9):994. https://doi.org/10.3390/d15090994
Chicago/Turabian StyleJäch, Manfred A., Michaela Brojer, Vlatka Mičetić Stanković, Marija Bošnjak, Dafna Luz, Netta Dorchin, Yaron Hershkovitz, Boris Novaković, Ivana Živić, Wolfgang Dorfer, and et al. 2023. "Elmis syriaca (Kuwert, 1890) and E. zoufali (Reitter, 1910) (Coleoptera: Elmidae) confirmed as distinct species based on molecular data, morphology and geographical distribution" Diversity 15, no. 9: 994. https://doi.org/10.3390/d15090994