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Article

Phenotypic and Molecular Characterization of Yeast Diversity Associated to Postharvest Fermentation Process of Coffee Fruits in Southern Ecuador

by
David Apolo
1,*,
José Miguel Fernández
2,
Ángel Benítez
1,3,
Jorge G. Figueroa
3,
Karla Estrada
1,3 and
Darío Cruz
1,3,*
1
Carrera de Biología, Universidad Técnica Particular de Loja, San Cayetano Alto s/n C.P., Loja 1101608, Ecuador
2
Departamento de Química, Research Group Procesos Innovadores Para el Desarrollo y Calidad de Productos y Alimentos Saludables, Universidad Técnica Particular de Loja, San Cayetano Alto s/n C.P., Loja 1101608, Ecuador
3
MS2E Research Group, Departamento de Ciencias Biológicas y Agropecuarias, Universidad Técnica Particular de Loja, San Cayetano Alto s/n C.P., Loja 1101608, Ecuador
*
Authors to whom correspondence should be addressed.
Diversity 2023, 15(9), 984; https://doi.org/10.3390/d15090984
Submission received: 8 July 2023 / Revised: 14 August 2023 / Accepted: 21 August 2023 / Published: 31 August 2023
(This article belongs to the Special Issue Biodiversity and Ecology of Soil Fungal Communities)
Browse Figures
Versions Notes

Abstract

:
Coffee (Coffea arabica), produced and marketed in Ecuador and worldwide, can be organoleptically improved by means of microorganisms such as well-characterized yeasts. This study aimed to isolate and characterize yeasts from three postharvest fermentation processes (i.e., Natural aerobic at room temperature; Carbonic maceration with a CO2 atmosphere at room temperature; and Carbonic refrigerated maceration with a CO2 atmosphere to 10 °C) of coffee fruits in Ecuador. Phenotypic and molecular analyses were conducted on 329 yeast isolates obtained from coffee farms in Loja, Olmedo, and Gonzanamá. Three universal media were used for yeast isolation diversity, and phenotypic characterization included morphology, sugar fermentation, salt tolerance, and ethanol resistance. Molecular characterization involved DNA analysis. The isolated diversity was classified into 12 morphotypes, nine distinct biochemical groups and nine genetic species. Only six species (i.e., Kurtzmaniella quercitrusa, Hanseniaspora opuntiae, Pichia. kluyveri, Torulaspora delbrueckii, T. quercuum, and Wickerhamomyces anomalus) identified phylogenetically corresponded to the designated morphotypes. But surprisingly, nine genetic species matched with the nine biochemical groups determined phenotypically analyzed using principal component analysis (PCA). Most of this diversity was found in the coffee plantation located in Gonzanamá, in contrast to Olmedo and Loja, without statistical significance (p value: 0.08295). On the other hand, the richness is not similar statistically (p value: 0.02991) between postharvest fermentation treatments. The findings suggest that the application of biochemical tests is useful for species determination, although morphological data may be ambiguous. Notably, Pichia kluyveri, detected in this study, holds potential for biotechnological evaluation in coffee fermentation processes.

1. Introduction

Coffee is currently one of the most traded products worldwide, as it is one of the main agro-export products in Ecuador [1]. In total, 20 of the country’s 24 provinces cultivate commercial coffee species: 135,466.2 ha are occupied by arabica varieties (Coffea arabica L.) and 63,748.8 ha are occupied by robusta (Coffea canephora Pierre.) [2]. The Ecuadorian region produces quality coffee, but its chemical and organoleptic improvements are sought through fermentative processes (beneficiation) prior to drying and milling [3].
In beneficiation processes, microorganisms such as yeasts play important roles as facilitators in speed and conversion of organic compounds, helping coffee beans to improve their organoleptic properties (taste and aroma) [4]. Fermentation involves several catabolic processes where organic substances are oxidized, transforming sugars into energy and other simple compounds such as ethanol, acetic acid, lactic acid, and butyric acid [5]. Usually, the farmers apply a typical or natural fermentation method after the harvest where the coffee fruits are dried after being cleaned [4], and other farmers employ carbonic maceration, an analogous technique to the wine fermentation process, where the coffee grains undergo fermentation within a medium saturated with carbon dioxide (CO2), generally maintained at room temperature or below 10 °C [5,6].
Microorganisms such as yeasts, a group of unicellular fungi with or without the presence of hyphae or pseudohyphae, act strongly in that fermentation processes [7]. Ecologically, the yeasts are present in a diverse range of habitats, including soil, aquatic environments, plant surfaces, foods, and skin and mucosal surfaces of animal hosts, but the soil environments represent the major ecological niche for fungi including yeast [8], helping in metabolism and facilitating carbon assimilation of fruits [9].
However, the diversity of microorganisms, especially yeasts, has been reported using molecular techniques [10], especially next-generation sequencing methods (i.e., metagenomics), useful mainly for diversity estimates using operational taxonomic units [11]. Likewise, the nuclear ribosomal DNA region ITS-5.8S is one of the most widely used regions as a DNA barcode [12], as well as the D1/D2 region of the 26S gene [13]. Also, yeast species can be determined by biochemical tests (mandatory to describe new species) through their fermentative capacity, as well as resistance to ethanol, NaCl, or germ tube generation tests [9]. The diversity of yeasts in coffee still requires exploration and adequate taxonomic characterization, as well as their ecological or biotechnological functionality in fermentative processes of fruits such as coffee [1,14,15].
Worldwide, the use of yeasts to improve organoleptic properties is gaining popularity, modifying the flavor and potential of the coffee bean with the use of species such as Saccharomyces cerevisiae and Pichia kluyveri [16,17]. However, in Ecuador and in the province of Loja renowned for its quality coffee [18], studies on improvements in coffee varieties and coffee pathogenic microorganisms are recorded [19]. However, in other localities, biotransformation of coffee flavor is proposed with the use of yeasts such as Saccharomyces cerevisiae and Pichia kluyveri through inoculations [6].
Now, very little is known about the biodiversity of yeasts associated with coffee fermentation processes on Ecuador, with only the study by Jumbo and Martínez [20], which evaluates the capacity of the Saccharomyces cerevisiae species to improve the chemical and organoleptic properties of coffee.
Due to the lack of information on the culturable diversity of yeasts from coffee in this region, the present research sought to isolate, phenotypically and molecularly characterize yeasts from three fermentation processes of coffee fruits (i.e., Natural aerobic at room temperature; Carbonic maceration with CO2 atmosphere at room temperature; and Carbonic refrigerated maceration with CO2 atmosphere to 10 °C) in three different farms in the province of Loja applied by local farmers to improve the sensory properties of coffee. In addition, the conservation of pure strains can be evaluated in the future in controlled fermentation processes of coffee, and the effect on the chemical and sensory properties of the final beverage can be determined.

2. Materials and Methods

2.1. Sampling

Nine samples of coffee beans were randomly collected from coffee farms located in the cantons (Figure 1, Table 1): Loja, Olmedo and Gonzanamá, collecting beans of the Geisha variety of Coffea arabica, characterized mainly by its slender trunk, elongated leaves and branches pointing towards the sky [21]. It is a variety of coffee growing around the Andes Mountain range known as shade coffee, and it is considered to be the best-quality coffee in the region [18].

2.2. Postharvest Fermentation Process

Coffee fruits (Coffea arabica L.) of the Geisha variety were collected in three farms at different altitudes (1230, 1500, 2010 m a.s.l) located in Loja province of Ecuador. The harvest was conducted by hand, and only ripe fruits were used. The samples were cleaned under continuous flow of tap water [3]. Then, the coffee fruits were placed in 4 L polyethylene bottles and fermented for 144 h under three different conditions: (a) Natural or “blank sample” aerobic at room temperature (N); (b) Carbonic maceration with a CO2 atmosphere at room temperature (CM); and (c) Carbonic refrigerated maceration with a CO2 atmosphere up to 10 °C (CRM). The atmospheric air in the bottles was constantly monitored by a gas analyzer (Oxybaby M+, Dortmund, Germany). Immediately after the fermentation process was completed, the microbiological analyses were carried out [5].

2.3. Yeast Isolation

In total, 10 g of each sample was inoculated into a sterilized stock solution (200 mL) of Yeast Peptone Dextrose Broth (YPD-DIFCO) and incubated at 27 °C for 24 h with continuous motion at 50 rpm [22].
After the incubation, a volume of 30 µL of the stock culture was seeded by a depletion technique on three solid media: Yeast Mold Agar (YMA-DIFCO), Sabouroad Dextrose Agar (SDA-DIFCO) and Yeast Extract Glucose agar (YGC) using Floran (FAVETEX) as a broad-spectrum antibiotic. The cultures were also incubated at 27 °C for 1–2 days. Subsequently, a random number (between 10 and 20 colonies) was selected for replication and purification on Potato dextrose Agar (PDA-DIFCO) solid medium plus Floran antibiotic at a final concentration of 1%. This process was repeated at 48 and 72 h [23].

2.4. Phenotypic Characterization of Pure Strain Sampling

Morphology: Strains were classified by morphotypes (MT) according to their growth form based on outline, size, coloration (British Standard Specification for colors) and aroma [20]. In addition, yeast shape and size were microscopically checked using an Olympus CX41 optical microscope and a 100× magnification. Preparations were performed under direct staining with 1% Phloxine B and 10% KOH.
Biochemistry: Biochemical tests correspond to (a) Sugar fermentation (differential with positive red and negative yellow staining) on a YPD-DIFCO culture medium (5 mL of medium plus 1.6 mL of methyl red prepared at 100 ppm) [21]; (b) Tolerance to NaCl at 10 and 15% (positive when there is colony growth) in a YMA medium under depletion stress and incubation at 30 °C (overnight); (c) Resistance to ethanol in a YPD medium, two tubes for each strain, one with 4.5 mL plus 0.5 mL of 96% ethanol and another 4.25 mL plus 0.75 mL of 96% ethanol, incubated at 30 °C with continuous movement at 60 rpm [24].
Additionally, a yeast germ tube test was performed to determine whether any yeast species corresponded to Candida albicans. All pure strains were tested by inoculating them in human serum and incubating at 37 °C for 2 h [25].

2.5. Statistical Analysis of Ordination and Analysis of Isolates by Farms

To determine differences between yeast species richness by location and treatment, we used one-way analysis of variance (ANOVA). We tested the normality of distributions of richness using the Shapiro–Wilk test (p value > 0.05).
Principal component analysis was performed based on the presence of each yeast population recovered to visualize the grouping of biochemical characteristics by species according to the characteristics of fermentation tests based on the Jaccard similarity index. Principal component analysis was performed using freely available statistical analysis PAST software version 4.10 [26]. We also computed a non-metric MDS (multidimensional scaling) ordination from the species to reveal the degree of similarity among treatments. We used the Euclidean distance as a metric for species similarity.

2.6. DNA Extraction, Amplification and Sequencing

A total of 36 strains were selected to be molecularly worked up due to similar characteristics found in the phenotypic analysis of the strains. Three strains from the same morphotypes, MT, were chosen. One colony for each selected strain was used for DNA extraction and amplification using the Phire Plant Direct PCR Master Mix commercial kit (Thermo Scientific, Vilna, Lithuania) according to the manufacturer’s specifications. The ITS-5.8S region of DNArn and partial LSU (D1/D2) was amplified with the following primers: ITS1F 5′ CTGGTCATTTAGAGGAAGTAA 3′ [27] and NL4 5′ GGTCCGTGTTTCAAGACGG 3′ [28]. PCR conditions were as follows: an initial denaturation at 98 °C for 5 min, followed by 45 cycles of denaturation at 98 °C for 10 s; banding at 55 °C for 10 s and extension at 72 °C for 30 s, and a final extension at 72 °C for 5 min.
PCR results were evaluated by 1% agarose gel electrophoresis and 1X GelRED staining. Positive products were purified using the PureLinkTM Quick PCR Purification Kit (Invitrogen, Vilna, Lithuania) and sequenced with the same set of PCR primers at Macrogen (Seoul, Republic of Korea).

2.7. Phylogenetic Analysis

The sequences obtained were visualized and edited in CodonCode Aligner 9.0.2 software (CodonCode Corporation, Centerville, MA, USA). The concatenated sequences (forward and reverse) were compared in GenBank Blast (https://www.ncbi.nlm.nih.gov/genbank/ accessed on 22 June 2023) to download the most similar sequences, preferably with taxonomic value (assigned species names). All sequences (37 new and 35 from GenBank) were aligned in MAFFT Version 7.489 using the G-INS-i strategy [29].
Two phylogenetic trees were performed under Maximum Likelihood algorithms [30], Kimura-2 parameter model and G + I nucleotide substitution rate model, followed by 1000 Bootstrap replicates employing MEGA 11 software [31].

2.8. Strain Preservation

The pure strains obtained were inoculated in a solution of 1.64 mL YPD medium plus 20% glycerol (permeable cryoprotectant) and allowed to grow for 1 h at 27 °C. Subsequently, they were frozen at −80 °C in cryovials. Additionally, yeast suspensions were made in 2 mL of triple-sterilized distilled water and 2 mL of 100% sterile mineral oil, which were kept refrigerated at 4 °C [32,33].

3. Results

3.1. Phenotyping and Diversity

A total of 329 pure strains were obtained and classified into 12 morphotypes (MT) (Figure 2). Several MT correlated by shape: rounded (MT1, MT3 and MT5) differentiated by attachments such as protrusions (MT5), visualization of vacuoles (MT1) and visualization of cell walls and membranes typical of the genera Torulaspora and Wickerhamomyces. On the other hand, oval-type morphology was found in MT2, MT6 and MT10, typical of the genera Kurztmaniella, Hanseniaspora and Pichia, respectively. Furthermore, elongated morphotypes (MT4, MT7, MT8, MT9 and MT11) were classified as recurrent in the genus Pichia; finally, protrusions attached to a rectangular oval MT (MT12) were found. Sporulation of MT2 strain BMF4L2 was recorded (Figure 2), and four internal segments were differentiated in the yeast.
Most of the strains isolated and macroscopically checked showed similar characteristics in terms of colony shape and odor.
The morphotype designated for Kurtzmaniella quercitrusa clustered with similar biochemical properties to those of Pichia fermentans, Hanseniaspora opuntiae and Torulaspora delbrueckii (Figure 3), standing out from the others by the presence of a germ tube, as well as growth in lactose and 15% NaCl. P. kluyveri, H. uvarum and Wickerhamomyces anomalus showed fermentation affinity in glucose and maintained a higher resistance in 10% NaCl.
Most species diversity was found in the coffee plantation located in Gonzanamá (Figure 4), in contrast to Olmedo and Loja, but statistically not significant (p value: 0.08295) (Table 2, Figure 4). In the isolates from Olmedo and Loja, the species Hanseniaspora opontuiae was not identified (Table 3). On the other hand, the isolates from Loja “El aguacate” (Table 1) presented a lower number of species that did not include Kurtzmaniella quercitrusa and Torulaspora delbrueckii, but presented a higher abundance for the genus Pichia (Table 2, Figure 4).
The richness was not similar between treatments (p value: 0.02991), which was statistically different from the natural fermentation, with a dispersion like the mean F: 4.077 (Figure 5A,B).
Biochemically from 329 strains, nine groups corresponding to the species Hanseniaspora opuntiae, H. uvarum, Kurtzmaniella quercitrusa, Pichia fermentans, P. kluyveri, P. membranifaciens, Torulaspora delbrueckii, T. quercuum and Wickerhamomyces anomalus were determined based on the description of Kurtzman et al. (2011).
Kurtzmaniella quercitrusa, Hamniospora opuntiae, P. fermentans and Torulaspora delbrueckii showed higher fermentation in glucose and lactose. In addition, they grew on media with NaCl up to 15% and 15% ethanol; this differed from P. kluyveri, which maintained similar tolerance characteristics up to 10% of each reagent.

3.2. Molecular Species Definition

From 36 molecularly worked-up strains, 60 (ITS-5.8S plus D1/D2 partial LSU) sequences (forward and reverse) were obtained. Twelve sequences were discarded due to multiple peaks in the chromatograms. All concatenated sequences (37 in total, Table 3) corresponded to the Ascomycota division (Table 3). Nine genetic species were determined (Table 3, Figure 6). The species Pichia membranifaciens was considered the most recurrent with 15 sequences and strains clustered in one clade (Figure 6).
The nine genetic species (Figure 6) corresponded to the nine biochemically determined phenotypes (i.e., Hanseniaspora opuntiae, H. uvarum, Kurtzmaniella quercitrusa, Pichia fermentans, P. kluyveri, P. membranifaciens, Torulaspora delbrueckii, T. quercuum and Wickerhamomyces anomalus) (Figure 2 and Figure 3). On the other hand, the nine genetic species only corresponded to six MTs where MT1 matched with Torulaspora delbrueckii; MT2 to Kurtzmaniela quercitrusa; MT3 to Wickerhamomyces anomalus; MT5 to Torulaspora quercuum; MT9 to Hanseniaspora opuntiae and MT10 matched with Pichia kluyveri.

4. Discussion

The diversity of microorganisms in different ecosystems is high and still unknown [1]. Also, not all microorganisms are culturable. However, 329 strains were isolated in this study from coffee fruits corresponding to 12 morphotypes, suggesting 12 morphospecies. Moreover, nine biochemical groups corresponding to the species Hanseniaspora opuntiae, H. uvarum, Kurtzmaniella quercitrusa, Pichia fermentans, P. kluyveri, P. membranifaciens, Torulaspora delbrueckii, T. quercuum, and Wickerhamomyces anomalus (Figure 3 and Figure 5) were determined.
Surprisingly, all these species identified biochemically are of the same genetic species determined phylogenetically. Nonetheless, only six of the designated morphospecies (Kurtzmaniella quercitrusa, Hanseniaspora opuntiae, Pichia. kluyveri, Torulaspora delbrueckii, T. quercuum, and Wickerhamomyces anomalus) corresponded to the genetic species. This is an indication that morphological characters are not entirely conclusive for defining yeast species [48] or other fungal species [24]. This is due to the variability that microorganisms can generate depending on the environment in which they develop [50]. In this regard, rapid and accurate identification of yeasts can be more effective using molecular tools [11], such as metagenomics [10].
On the other hand, it is well known that biochemical tests are an essential tool for the description of microorganisms [51] and are widely used in the description of yeast species such as within the genus Candida [52]. Likewise, De Melo Pereira et al. [53] indicate that fermentative capacity are unique characteristics to define species within different genera like Saccharomyces into Saccharomycotina. Biochemically and morphologically, the MT2 was determined as the species Candida quercitrusa, actually synonymized with Krutzmaniella quercitrusa [34]. The genetic species determined as Pichia membranifaciens (98% Bootstrap) contains several sequences from strains M2L7, MF4L8, BMF1L6, AC1L5, BMF1L2, BMF1L5, BMF4L9, AC3L3, MF1L3, MF1L7, AC2L2, AF2L6, MC1L9, BMF1L9 and MF4L5, which were preliminarily classified into two morphotypes, MT7 and MT12, respectively (Figure 2). It is likely that the morphological variation corresponds to an adaptation of each strain to the culture medium, as suggested for other yeasts [54].
Of the species isolated in this study, the species Torulaspora delbruekii and T. quercuum have been previously reported in studies of fungal diversity associated with coffee in the species Coffea arabica L. in different locations of the planet such as USA, Europe and Asia, but no records of these species were found in Ecuador [54,55]. These species are considered to be sisters, as they are grouped within the same phylogenetic clade [15], accompanied by morphological similarity as indicated in this study (Figure 2 (MT1 and MT5)). Additionally, similar results in NaCl, ethanol resistance, malt and lactose fermentation were found for Torulaspora delbruekii and T. quercuum, contributing to the evidence of their taxonomic closeness.
Similarly, the species Krutzmaniella quercitrusa, Hanseniaspora opuntiae, Pichia kluyveri, and Torulaspora delbrueckii [56,57] have been reported from coffee fruits in nearby countries such as Brazil, Colombia and Chile. In addition, isolation of yeasts from coffee (Coffea arabica) belonging to the genera Pichia, Candida and Saccharomycopsis has been reported [58,59]. For the southern region of Ecuador, the nine genetic species described in this study and species such as Candida albicans and Saccharomyces cerevisiae have been reported in a study on the microbiome in the beverage called colada morada [60]. It is known that the diversity of microorganisms can vary due to environmental factors [55] such as fermentation process in closed tanks with airlocks used by the farmers to generate new sensory profiles.
Local farmers apply similar fermentation processes as evaluated here: natural fermentation or controlled atmosphere with a CO2-rich environment at room temperature and refrigeration to improve sensory profiles mainly conducted by development of different microorganisms like yeasts [56,57]. Biotechnologically, yeasts such as those determined in this study, for example, Pichia membranifaciens, are applicable in fermentation processes of fruits such as coffee [58,59,61], or as biocontrollers of fungal pathogens (e.g., Botrytis cinerea) [62]. Likewise, species such as Pichia fermentans and P. Kluyveri have been used in the wine and brewing industry including various fruits such as coffee [61]; likewise, Hanseniaspora uvarum and Wickerhamomyces anomalus have been used to date in the wine industry, increasing their organoleptic properties such as aroma and flavors [60].
On the other hand, the species Torulaspora delbrueckii, a yeast with remarkable resistance to osmotic and freezing stress [53], possesses flavor- and aroma-enhancing properties in wine, beer, or bread dough fermentation processes [58]. This yeast is considered a biotechnological model that can be used in food industries [53]. Kurtzmaniella quercitrusa and Hanseniaspora opuntiae species have been used and reported in the fermentation of cocoa beans in Malaysia [14]. In the case of Torulaspora quercuum, it has been reported in cider fermentation in association with other yeasts described in this study [15]. However, its metabolic potential is currently being studied, and it is defined as a potential biotechnological model in the production of ethanol associated with microalgae [63].
Future studies are required to determine the capabilities and behavior of these yeasts in coffee fermentation processes and the effect on the chemical and sensory properties of the final beverage.

5. Conclusions

The diversity of yeast can vary due to environmental factors such as fermentation process, as is evaluated here (i.e., Natural aerobic at room temperature; Carbonic maceration with CO2 atmosphere at room temperature; and Carbonic refrigerated maceration with CO2 atmosphere to 10 °C), allowing the obtention of culturable yeast. This diversity after biochemical and molecular test is represented by nine species, Hanseniaspora opuntiae, H. uvarum, Kurtzmaniella quercitrusa, Pichia fermentans, P. kluyveri, P. membranifaciens, Torulaspora delbrueckii, T. quercuum, and Wickerhamomyces anomalus. These nine species determined biochemically and molecularly have congruent correspondence, contrary to morphological characters that can be ambiguous due to overlapping between species as, for example, within the genus Pichia.
The nine species determined here are considered new Saccharomycotina records for southern Ecuador according to the revised literature.

Author Contributions

D.C., D.A., Á.B. and J.M.F. contributed to the conceptualization and methodology of this study. J.M.F. and J.G.F. carried out sampling, and critical revision on manuscript, K.E., contributed with laboratory work and general comments on manuscript. All authors contributed with their experience to improve the whole manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded with five thousand American dollars (USD 5000) into the internal project PROY_INV_QUI_2021_2837 by the Vicerrectorado de Investigación of the Universidad Técnica Particular de Loja.

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Not Applicable.

Acknowledgments

The authors would like to thank some students at the Biology career for help during the laboratory work.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Geographical location of the coffee farms sampled in the province of Loja.
Figure 1. Geographical location of the coffee farms sampled in the province of Loja.
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Figure 2. Morphotypes determined for the yeast isolates in this study. Photographs indicate reddish staining by Phloxine 1%. Illustrations represent in detail the yeast forms.
Figure 2. Morphotypes determined for the yeast isolates in this study. Photographs indicate reddish staining by Phloxine 1%. Illustrations represent in detail the yeast forms.
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Figure 3. PCA for the nine species determined biochemically, according to the fermentation tests on (1) malt, (2) sucrose, (3) glucose, (4) lactose. Tolerance to (5) 10% NaCl, (6) 15% NaCl, (7) 10% ethanol, (8) 15% ethanol. (9) Germ Tube Test.
Figure 3. PCA for the nine species determined biochemically, according to the fermentation tests on (1) malt, (2) sucrose, (3) glucose, (4) lactose. Tolerance to (5) 10% NaCl, (6) 15% NaCl, (7) 10% ethanol, (8) 15% ethanol. (9) Germ Tube Test.
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Figure 4. Diversity analysis by sampling site. The numbers on abundance axis represents isolates by each of the nine species.
Figure 4. Diversity analysis by sampling site. The numbers on abundance axis represents isolates by each of the nine species.
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Figure 5. (A) NMDS of the species found according to the treatment: x = Natural or “blank sample” aerobic at room temperature (N); □ = Carbonic maceration with CO2 atmosphere at room temperature (CM); and • = Carbonic refrigerated maceration with CO2 atmosphere to 10 °C (CRM), (B) boxplot of the species according to the treatments.
Figure 5. (A) NMDS of the species found according to the treatment: x = Natural or “blank sample” aerobic at room temperature (N); □ = Carbonic maceration with CO2 atmosphere at room temperature (CM); and • = Carbonic refrigerated maceration with CO2 atmosphere to 10 °C (CRM), (B) boxplot of the species according to the treatments.
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Figure 6. Phylogenetic placement of ITS-5.8S plus D1/D2 LSU partial of yeast sequences. Maximum likelihood phylogenetic analysis, with Bootstrap values greater than 50, are presented above the nodes.
Figure 6. Phylogenetic placement of ITS-5.8S plus D1/D2 LSU partial of yeast sequences. Maximum likelihood phylogenetic analysis, with Bootstrap values greater than 50, are presented above the nodes.
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Table 1. Reference information of the samples evaluated for each treatment and farm.
Table 1. Reference information of the samples evaluated for each treatment and farm.
CantonFarms by SectorLatitude and LongitudeAltitude
(m.a.s.l.)		No Sample CodePostharvest Fermentation Process
LojaEl Aguacate3°55.848′ S 79°13.340′ W2010ANatural
ACCarbonic maceration with CO2 atmosphere at room temperature
AFCarbonic refrigerated maceration with CO2 atmosphere up to 10 °C
OlmedoPapá Café3°55.960′ S 79°38.729′ W1230BNatural
BMFCarbonic maceration with CO2 atmosphere at room temperature
BMCCarbonic refrigerated maceration with CO2 atmosphere up to 10 °C
GonzanamáEl Arenal4°06′02.0″ S 79°23′40.4″ W1500 mMNatural
MCCarbonic maceration with CO2 atmosphere at room temperature
MFCarbonic refrigerated maceration with CO2 atmosphere up to 10 °C
Table 2. ANOVA of the richness of yeasts found according to the fermentation treatment used and the origin of the sample.
Table 2. ANOVA of the richness of yeasts found according to the fermentation treatment used and the origin of the sample.
Sum of Squares DfMean Square FFp Value
Richness according to the type of fermentationBetween groups15.740727.870374.0770.02991
Within groups 46.3333241.93056
Total62.0741260.3307
Richness by locationBetween groups11.629625.814812.7670.08295
Within groups50.4444242.10185
Total62.0741260.08731
Table 3. Strains obtained and their BLAST reference information.
Table 3. Strains obtained and their BLAST reference information.
SpeciesCollection NumberMorphotype
Referential		GenBank Accession NumberCountryLiterature Reference
Kurztmaniella quercitrusaCM1L1MT7OR145791EcuadorThis study
Candida quercitrusaHA 1669-AM160627AustriaXiao [34]
Kurztmaniella quercitrusaBMF2L3MT7OR145790EcuadorThis study
Hannseniaspora opuntiaeMF3L2MT8OR145783EcuadorThis study
Hannseniaspora opuntiaeJEY258-KC111445TunisiaEddouzi et al. [35]
Hannseniaspora opuntiaeKOT1-KY849378TunisiaBen Taheur [36]
Hannseniaspora opuntiaeSM10UFAM-MN268780BrazilSilva et al. [37]
Hanseniaspora uvarumAC3L1MT9OR145784EcuadorThis study
Hanseniaspora uvarumP43C012-JX188164USABourret et al. [38]
Hanseniaspora uvarumMer383-MT734682SloveniaZabukovec et al. [39]
Krutzmaniella quercitrusaCBS 4412-MK394107NetherlandsStavrou et al. [40]
Krutzmaniella quercitrusaLHCT1-KX961193.ChinaLi et al. [41]
Nuerospora crassaAR14R-OK148118IndiaDirect submission
Nuerospora crassaI1-3-MH507014ChinaDirect submission
Nuerospora crassaI1-3-MK064500ChinaDirect submission
Pichia fermentansAF2L7MT4OR145756EcuadorThis study
Pichia fermentansCM1L6MT4OR145758EcuadorThis study
Pichia fermentansAC3L10MT4OR145755EcuadorThis study
Pichia fermentansAC4L2MT4OR145757EcuadorThis study
Pichia fermentansHR-1 DS1308-KM029994USADirect submission
Pichia fermentansHA-1-KU820951Republic of KoreaDirect submission
Pichia fermentansATCC 10651-GQ458040CanadáArteau et al. [42]
Pichia fermentansCBS 187-MK394169NetherlandsStavrou et al. [40]
Pichia kluyveriBMC4L9MT1OR145759EcuadorThis study
Pichia kluyveriAF2L3MT10OR145761EcuadorThis study
Pichia kluyveriBMF4L1MT10OR145762EcuadorThis study
Pichia kluyveriAF2L1MT10OR145760EcuadorThis study
Pichia kluyveriP25B004-JX188197USABourret et al. [38]
Pichia kluyveriH12-MN266784JapónVasques et al. [43]
Pichia kluyveriKBP:Y-4954-MG367291MoscowDirect Submission
Pichia kluyveriPMM10-1033666L-KP132502AustraliaIrinyi et al. [44]
Pichia kluyveriCBS 188-MK394165NetherlandsStavrou et al. [40]
Pichia membranifaciensM2L7MT6OR145770EcuadorThis study
Pichia membranifaciensMF4L8MT6OR145772EcuadorThis study
Pichia membranifaciensBMF1L6MT6OR145774EcuadorThis study
Pichia membranifaciensAC1L5MT10OR145767EcuadorThis study
Pichia membranifaciensBMF1L2MT6OR145769EcuadorThis study
Pichia membranifaciensBMF1L5MT6OR145775EcuadorThis study
Pichia membranifaciensBMF4L9MT12OR145773EcuadorThis study
Pichia membranifaciensAC3L3MT6OR145766EcuadorThis study
Pichia membranifaciensMF1L3MT6OR145776EcuadorThis study
Pichia membranifaciensMF1L7MT6OR145777EcuadorThis study
Pichia membranifaciensAC2L2MT11OR145764EcuadorThis study
Pichia membranifaciensAF2L6MT6OR145765EcuadorThis study
Pichia membranifaciensP43C010-JX188208USABourret et al. [38]
Pichia membranifaciensCBS 82-DQ198951ChinaWu et al. [45]
Pichia membranifaciensCBS 107-MK394163NetherlandsStavrou et al. [40]
Pichia membranifaciensMC1L9MT6OR145771EcuadorThis study
Pichia membranifaciensBMF1L9MT6OR145768EcuadorThis study
Pichia membranifaciensMF4L5MT12OR145763EcuadorThis study
Torulaspora delbrueckiiMC4L2MT2OR145788EcuadorThis study
Torulaspora delbrueckiiMC1L1MT2OR145787EcuadorThis study
Torulaspora delbrueckiiMC2L1MT2OR145789EcuadorThis study
Torulaspora delbrueckiiB2/III/17-HE799671SloveniaGolic et al. [46]
Torulaspora delbrueckiiCBS 1146-MK394138NetherlandsStavrou et al. [40]
Torulaspora quercuumBMF4L7MT3OR145786EcuadorThis study
Torulaspora quercuumAF2L8MT3OR145785EcuadorThis study
Torulaspora quercuumCBS:11403-KY105673NetherlandsVu et al. [47]
Torulaspora quercuumCGMCC AS 2-NR137029ChinaWang et al. [48]
Torulaspora quercuumXZ-46A-FJ888525ChinaWang et al. [48]
Torulaspora quercuumXZ19100-5-MW710147ChinaDirect Submission
Wickerhamomyces anomalusBMC2L7MT5OR145778EcuadorThis study
Wickerhamomyces anomalusAC4L9MT5OR145782EcuadorThis study
Wickerhamomyces anomalusBMF2L7MT5OR145779EcuadorThis study
Wickerhamomyces anomalusMF1L9MT5OR145780EcuadorThis study
Wickerhamomyces anomalusMC3L1MT5OR145781EcuadorThis study
Wickerhamomyces anomalusCBS 5759-MK394130NetherlandsStavrou et al. [40]
Wickerhamomyces anomalusI 29-HF952836NetherlandsZha et al. [49]
Wickerhamomyces anomalusVIT-ASN01-KX253664IndiaDirect Submission
Wickerhamomyces anomalusDBL01s1-LC120363JapanTanahashi and Hawes [50]
Wickerhamomyces anomalusiL-51-2-FN868149GermanyGlushakova et al. [51]
Wickerhamomyces anomalusCBS 5759-MH545921NetherlandsStavrou et al. [40]
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Apolo, D.; Fernández, J.M.; Benítez, Á.; Figueroa, J.G.; Estrada, K.; Cruz, D. Phenotypic and Molecular Characterization of Yeast Diversity Associated to Postharvest Fermentation Process of Coffee Fruits in Southern Ecuador. Diversity 2023, 15, 984. https://doi.org/10.3390/d15090984

AMA Style

Apolo D, Fernández JM, Benítez Á, Figueroa JG, Estrada K, Cruz D. Phenotypic and Molecular Characterization of Yeast Diversity Associated to Postharvest Fermentation Process of Coffee Fruits in Southern Ecuador. Diversity. 2023; 15(9):984. https://doi.org/10.3390/d15090984

Chicago/Turabian Style

Apolo, David, José Miguel Fernández, Ángel Benítez, Jorge G. Figueroa, Karla Estrada, and Darío Cruz. 2023. "Phenotypic and Molecular Characterization of Yeast Diversity Associated to Postharvest Fermentation Process of Coffee Fruits in Southern Ecuador" Diversity 15, no. 9: 984. https://doi.org/10.3390/d15090984

APA Style

Apolo, D., Fernández, J. M., Benítez, Á., Figueroa, J. G., Estrada, K., & Cruz, D. (2023). Phenotypic and Molecular Characterization of Yeast Diversity Associated to Postharvest Fermentation Process of Coffee Fruits in Southern Ecuador. Diversity, 15(9), 984. https://doi.org/10.3390/d15090984

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