Next Article in Journal
Comparison of Creatinine-, Cystatin C-, and Combined Creatinine–Cystatin C-Based Equations for Estimating Glomerular Filtration Rate: A Real-World Analysis in Patients with Chronic Kidney Disease
Previous Article in Journal
Postbiotics Combination Synergises the Antiproliferative Effects of Doxorubicin in Gastric Cancer Cells: A Cellular and Molecular Deep Dive
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 27
Issue 1
10.3390/ijms27010363
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Characteristics and Expression of RBX1 Gene Involved in Ovarian Development of Scylla paramamosain

by
Fengying Zhang
1,
Ting Huang
1,2,
Yuanhao Ren
1,
Ming Zhao
1,
Wei Wang
1,
Zhiqiang Liu
1,
Keyi Ma
1,
Yin Fu
1,
Wei Chen
1,
Lingbo Ma
1 and
Chunyan Ma
1,*
1
Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
2
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2026, 27(1), 363; https://doi.org/10.3390/ijms27010363
Submission received: 24 November 2025 / Revised: 19 December 2025 / Accepted: 26 December 2025 / Published: 29 December 2025
(This article belongs to the Section Molecular Genetics and Genomics)
Browse Figures
Versions Notes

Abstract

Ring Box Protein-1 (RBX1) is an essential component of the Skp1-cullin-F-box protein (SCF) E3 ubiquitin ligase, which is involved in the regulation of oocyte maturation in the form of ubiquitination substrate modification. In this study, a sequence of RBX1 (Sp-RBX1) was identified and analyzed using bioinformatics methods from the transcriptome data of Scylla paramamosain. The length of Sp-RBX1 cDNA sequence was 1247 bp, consisting of a 336 bp open reading frame (ORF). Sequence analysis revealed that the protein contained a C-terminal modified RING-H2 finger domain, with two zinc binding sites and a Cullin binding site, classifying it as a member of the RBX1 superfamily. The results of real-time fluorescence quantitative PCR (RT-qPCR) showed that Sp-RBX1 expression in the ovary was low at stages I and II, then significantly increased from stage III to V (p < 0.05), which indicated that it might be closely related to the maturation of oocytes. It also peaked at stage II in the hepatopancreas, then sharply declined from stages III to V. The expression pattern might be related to the accumulation of fat in the early development of hepatopancreas. Furthermore, we characterized the expression of Sp-RBX1 induced by follicle-stimulating hormone (FSH) and estradiol (E2) hormones. The results showed that the expression in the ovary was up-regulated by FSH and significantly inhibited by E2. The expression in the hepatopancreas increased only at 0.5 µmol/L concentration of FSH, and decreased in other groups. Conversely, it was up-regulated by E2. Thus, the expression of Sp-RBX1 was influenced by FSH in a concentration-dependent manner. These findings could offer valuable insights for further research on ovarian maturation in crustaceans.
Keywords: Scylla paramamosain; RBX1; oocyte maturation; ovary; hepatopancreas; development Scylla paramamosain; RBX1; oocyte maturation; ovary; hepatopancreas; development

Share and Cite

MDPI and ACS Style

Zhang, F.; Huang, T.; Ren, Y.; Zhao, M.; Wang, W.; Liu, Z.; Ma, K.; Fu, Y.; Chen, W.; Ma, L.; et al. The Characteristics and Expression of RBX1 Gene Involved in Ovarian Development of Scylla paramamosain. Int. J. Mol. Sci. 2026, 27, 363. https://doi.org/10.3390/ijms27010363

AMA Style

Zhang F, Huang T, Ren Y, Zhao M, Wang W, Liu Z, Ma K, Fu Y, Chen W, Ma L, et al. The Characteristics and Expression of RBX1 Gene Involved in Ovarian Development of Scylla paramamosain. International Journal of Molecular Sciences. 2026; 27(1):363. https://doi.org/10.3390/ijms27010363

Chicago/Turabian Style

Zhang, Fengying, Ting Huang, Yuanhao Ren, Ming Zhao, Wei Wang, Zhiqiang Liu, Keyi Ma, Yin Fu, Wei Chen, Lingbo Ma, and et al. 2026. "The Characteristics and Expression of RBX1 Gene Involved in Ovarian Development of Scylla paramamosain" International Journal of Molecular Sciences 27, no. 1: 363. https://doi.org/10.3390/ijms27010363

APA Style

Zhang, F., Huang, T., Ren, Y., Zhao, M., Wang, W., Liu, Z., Ma, K., Fu, Y., Chen, W., Ma, L., & Ma, C. (2026). The Characteristics and Expression of RBX1 Gene Involved in Ovarian Development of Scylla paramamosain. International Journal of Molecular Sciences, 27(1), 363. https://doi.org/10.3390/ijms27010363

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop