Review Reports - Recent Advances in Genome Editing and Bioinformatics: Addressing Challenges in Genome Editing Implementation and Genome Sequencing

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

I have the following comments;

The manuscript is about Bioinformatic Approach to Exploring Genome Editing Targets 2
through Pathway Design but the author did not focus on the title of the manuscript and describe some of the other aspects.
The author should focus of the bioinformatics tools and describe how it can help to identify the targets for genome editing and how off-target effects can be minimized.
Some of the sections need to be improved and expand and require more literature background and critical viewpoint.
The figures needs to be improved as some of the figures are very simple and not conveying any significant knowledge about the topic.
Also, there are some typos.

Author Response

Comments:

> I have the following comments;

> 1. The manuscript is about Bioinformatic Approach to Exploring Genome Editing Targets 2 through Pathway Design but the author did not focus on the title of the manuscript and describe some of the other aspects.

> 2. The author should focus of the bioinformatics tools and describe how it can help to identify the targets for genome editing and how off-target effects can be minimized.

Response:

Thanks for your helpful comment. As I wrote the review, my perspective changed from pathway design to the implementation of genome editing and genome sequencing that preceded that, I will change the title to “Recent Advances in Genome Editing and Bioinformatics: Addressing Challenges in Genome Editing Implementation and Genome Sequencing”. The references are revised to reduce the manuscript citations in Table 4 (currently Table 3).

Comments:

> 3. Some of the sections need to be improved and expand and require more literature background and critical viewpoint.

> 4. The figures needs to be improved as some of the figures are very simple and not conveying any significant knowledge about the topic.

Response:

Thanks for your comments. While the comments are so general that I cannot understand what part of the manuscript the reviewer is talking about, I reconsider the organization of figures and tables in the manuscript. According to reviewer’s comment, table 2 in the original version was removed to focus much on the main theme. I’m sure figures in the manuscript are original and informative enough for the reader of IJMS.

> 5. Also, there are some typos.

I recheck the manuscript and tried to fix the typos in the manuscript thoroughly.

Reviewer 2 Report

Comments and Suggestions for Authors

In this manuscript, the authors summarized genome sequencing and bioinformatics technologies. More about pathway design needed to be added.

As mentioned in the title, this is a review related to bioinformatic approach and pathway design. However, only a short paragraph is about pathway design. Can add more about this part, such as metabolic model and others.
AI technology have been applied in pathway design. Provide more samples.

Author Response

Comments 1:

> In this manuscript, the authors summarized genome sequencing and bioinformatics technologies. More about pathway design needed to be added.

> 1. As mentioned in the title, this is a review related to bioinformatic approach and pathway design. However, only a short paragraph is about pathway design. Can add more about this part, such as metabolic model and others.

Response 1:

Comments 2:

> 2. AI technology have been applied in pathway design. Provide more samples.

Response 2:

Thanks for your comment. I added one more sentence for the description of the use of AI in PFOCR in Line 574.

> PFOCR utilizes artificial intelligence (AI) for extracting gene names and chemical compounds from figure images in PMC.

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

The manuscript has been revised and improved significantly.