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Volume 26
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10.3390/ijms262311410
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

ESR1 Regulates Fecundity and Functions in Sheep Endometrial Stromal Cells

by
Kang Li
1,2,3,
Xiaoxiao Gao
4,
Zhibo Wang
1,3,
Dongxu Li
1,3,
Jiahe Guo
1,3,
Feng Wang
1,3,* and
Tianlong Guo
2,*
1
Sanya Research Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya 572000, China
2
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010030, China
3
Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China
4
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(23), 11410; https://doi.org/10.3390/ijms262311410
Submission received: 8 September 2025 / Revised: 20 November 2025 / Accepted: 22 November 2025 / Published: 25 November 2025
(This article belongs to the Special Issue Molecular Research on Reproductive Physiology and Endocrinology)
Versions Notes

Abstract

Endometrial stromal cell (ESC) plasticity is critical to embryo survival and conceptus elongation; however, their genetic regulation remains unclear. To elucidate the molecular mechanisms by which ESR1 regulates sheep fecundity through endometrial function, we investigated the effects of ESR1 on ovine ESCs in vitro. Tissue distribution analysis revealed elevated ESR1 expression in the reproductive organs, particularly the endometrium, compared with that in non-reproductive tissues. Both ESR1 mRNA and its encoded ERα protein were significantly upregulated in the endometrium of high-fecundity Hu sheep compared to those in the endometrium of low-fecundity Hu sheep. Given the specific localisation of ERα in ESCs, we examined the functional roles of ESR1 in these cells in vitro. ESR1 knockdown in ESCs inhibited cell proliferation and induced apoptosis, concomitant with increased Caspase3 and Caspase9 mRNA expression. Furthermore, ESR1 interference triggered G0/G1-phase cell cycle arrest via the upregulation of P53 and P21 and significantly impaired cell migration capacity. Collectively, these results demonstrate that ESR1 critically regulates ESC proliferation, apoptosis, cell cycle progression, and migration. Our findings establish ESR1 as a key determinant of sheep fecundity and provide mechanistic insights into the high reproductive efficiency of Hu sheep.
Keywords: ESR1; proliferation; apoptosis; migration; endometrial stromal cells ESR1; proliferation; apoptosis; migration; endometrial stromal cells

Share and Cite

MDPI and ACS Style

Li, K.; Gao, X.; Wang, Z.; Li, D.; Guo, J.; Wang, F.; Guo, T. ESR1 Regulates Fecundity and Functions in Sheep Endometrial Stromal Cells. Int. J. Mol. Sci. 2025, 26, 11410. https://doi.org/10.3390/ijms262311410

AMA Style

Li K, Gao X, Wang Z, Li D, Guo J, Wang F, Guo T. ESR1 Regulates Fecundity and Functions in Sheep Endometrial Stromal Cells. International Journal of Molecular Sciences. 2025; 26(23):11410. https://doi.org/10.3390/ijms262311410

Chicago/Turabian Style

Li, Kang, Xiaoxiao Gao, Zhibo Wang, Dongxu Li, Jiahe Guo, Feng Wang, and Tianlong Guo. 2025. "ESR1 Regulates Fecundity and Functions in Sheep Endometrial Stromal Cells" International Journal of Molecular Sciences 26, no. 23: 11410. https://doi.org/10.3390/ijms262311410

APA Style

Li, K., Gao, X., Wang, Z., Li, D., Guo, J., Wang, F., & Guo, T. (2025). ESR1 Regulates Fecundity and Functions in Sheep Endometrial Stromal Cells. International Journal of Molecular Sciences, 26(23), 11410. https://doi.org/10.3390/ijms262311410

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