Predictive Factors for Cervical Intraepithelial Neoplasia in Women with Abnormal Cytology According to Human Papillomavirus Genotype: An Observational Study
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Population and Eligibility Criteria
2.3. Study Variables
- HPV genotype: categorized as negative, HPV16, HPV18, or other high-risk HPV genotypes.
- Cytology: classified as ASC-US, ASC-H, AGC, LSIL, or HSIL.
- Age: grouped into three categories: 20–29, 30–59, and ≥60 years.
2.4. Diagnostic Procedures
2.5. Statistical Analysis
2.6. Sample Size Calculation
2.7. Ethical Considerations
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
List of Abbreviations
HPV | Human papillomavirus |
hrHPV | High-risk human papillomavirus |
CIN | Cervical intraepithelial neoplasia |
CIN2–3 | Cervical intraepithelial neoplasia grades 2 and 3 |
ASC-US | Atypical squamous cells of undetermined significance |
ASC-H | Atypical squamous cells—cannot exclude HSIL |
AGC | Atypical glandular cells |
LSIL | Low-grade squamous intraepithelial lesion |
HSIL | High-grade squamous intraepithelial lesion |
aOR | Adjusted odds ratio |
OR | Odds ratio |
CI | Confidence interval |
GLM | Generalized linear model |
LLETZ | Large loop excision of the transformation zone |
STROBE | Strengthening the Reporting of Observational Studies in Epidemiology |
AIC | Akaike information criterion |
ASCCP | American Society for Colposcopy and Cervical Pathology |
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Variable | n (%) |
---|---|
Age group (years) | |
20–29 | 25 (13.2) |
30–59 | 145 (76.7) |
≥60 | 19 (10.1) |
Parity | |
Nulliparous | 42 (22.2) |
Multiparous | 147 (77.8) |
Cytology | |
ASC-H | 22 (11.6) |
ASC-US | 68 (36.0) |
AGC | 8 (4.2) |
HSIL | 38 (20.1) |
LSIL | 53 (28.0) |
HPV status | |
HPV16 | 46 (24.3) |
HPV18 | 4 (2.1) |
Other HR-HPV | 139 (73.6) |
Total | 189 (100) |
Category | HPV16, n (%) | HPV18, n (%) | Other HR-HPV, n (%) | Total, n (%) | * p-Value |
---|---|---|---|---|---|
Cytology | |||||
ASC-H | 5 (22.7) | 1 (4.5) | 16 (72.7) | 22 (100) | |
ASC-US | 13 (19.1) | 1 (1.5) | 54 (79.4) | 68 (100) | |
AGC | 0 (0.0) | 0 (0.0) | 8 (100.0) | 8 (100) | |
HSIL | 18 (47.4) | 2 (5.3) | 18 (47.4) | 38 (100) | |
LSIL | 10 (18.9) | 0 (0.0) | 43 (81.1) | 53 (100) | |
Subtotal | 46 (24.3) | 4 (2.1) | 139 (73.5) | 189 (100) | 0.009 |
Biopsy | |||||
Negative | 18 (16.2) | 2 (1.8) | 91 (82.0) | 111 (100) | |
CIN1 | 5 (20.0) | 0 (0.0) | 20 (80.0) | 25 (100) | |
CIN2 | 6 (40.0) | 0 (0.0) | 9 (60.0) | 15 (100) | |
CIN3 | 17 (44.7) | 2 (5.3) | 19 (50.0) | 38 (100) | |
Subtotal | 46 (24.3) | 4 (2.1) | 139 (73.5) | 189 (100) | 0.005 |
Age Group (years) | Negative, n (%) | CIN1, n (%) | CIN2, n (%) | CIN3, n (%) | Total, N (%) | * p-Value |
---|---|---|---|---|---|---|
20–29 | 18 (72.0) | 4 (16.0) | 2 (8.0) | 1 (4.0) | 25 (100) | |
30–59 | 84 (57.9) | 19 (13.1) | 11 (7.6) | 31 (21.4) | 145 (100) | |
≥60 | 9 (47.4) | 2 (10.5) | 2 (10.5) | 6 (31.6) | 19 (100) | |
Total | 111 (58.7) | 25 (13.2) | 15 (7.9) | 38 (20.1) | 189 (100) | 0.401 |
Cytology Category | Negative, n (%) | CIN1, n (%) | CIN2, n (%) | CIN3, n (%) | Total, n (%) | p-Value |
---|---|---|---|---|---|---|
ASC-H | 14 (63.6) | 1 (4.5) | 1 (4.5) | 6 (27.3) | 22 (100) | |
ASC-US | 55 (80.9) | 10 (14.7) | 1 (1.5) | 2 (2.9) | 68 (100) | |
AGC | 3 (37.5) | 4 (50.0) | 0 (0.0) | 1 (12.5) | 8 (100) | |
HSIL | 2 (5.3) | 0 (0.0) | 9 (23.7) | 27 (71.1) | 38 (100) | |
LSIL | 37 (69.8) | 10 (18.9) | 4 (7.5) | 2 (3.8) | 53 (100) | |
Total | 111 (58.7) | 25 (13.2) | 15 (7.9) | 38 (20.1) | 189 (100) | <0.001 |
Factor | Univariable OR (95% CI) | p-Value | Multivariable aOR (95% CI) | p-Value |
---|---|---|---|---|
Age ≥ 30 | 3.65 (1.66–8.01) | 0.001 | 4.50 (1.90–10.65) | 0.001 |
HPV16+ | 3.63 (1.76–7.50) | <0.001 | 4.19 (1.95–9.00) | <0.001 |
HPV18+ | 3.30 (0.45–24.1) | 0.239 | – | – |
Other HR-HPV+ | 0.28 (0.13–0.56) | <0.001 | – | – |
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Medina Bueno, G.A.; Jaramillo Saavedra, E.A.; Torres Rendón, N.; Huareccallo Suni, D.D. Predictive Factors for Cervical Intraepithelial Neoplasia in Women with Abnormal Cytology According to Human Papillomavirus Genotype: An Observational Study. Int. J. Mol. Sci. 2025, 26, 9612. https://doi.org/10.3390/ijms26199612
Medina Bueno GA, Jaramillo Saavedra EA, Torres Rendón N, Huareccallo Suni DD. Predictive Factors for Cervical Intraepithelial Neoplasia in Women with Abnormal Cytology According to Human Papillomavirus Genotype: An Observational Study. International Journal of Molecular Sciences. 2025; 26(19):9612. https://doi.org/10.3390/ijms26199612
Chicago/Turabian StyleMedina Bueno, Gonzalo Arturo, Enrique Adolfo Jaramillo Saavedra, Natalia Torres Rendón, and Damaris Diana Huareccallo Suni. 2025. "Predictive Factors for Cervical Intraepithelial Neoplasia in Women with Abnormal Cytology According to Human Papillomavirus Genotype: An Observational Study" International Journal of Molecular Sciences 26, no. 19: 9612. https://doi.org/10.3390/ijms26199612
APA StyleMedina Bueno, G. A., Jaramillo Saavedra, E. A., Torres Rendón, N., & Huareccallo Suni, D. D. (2025). Predictive Factors for Cervical Intraepithelial Neoplasia in Women with Abnormal Cytology According to Human Papillomavirus Genotype: An Observational Study. International Journal of Molecular Sciences, 26(19), 9612. https://doi.org/10.3390/ijms26199612