Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells

Abramicheva, Polina A.; Sokolov, Ilya A.; Druzhinina, Arina A.; Potashnikova, Daria M.; Andrianova, Nadezda V.; Semenovich, Dmitry S.; Manskikh, Vasily N.; Zorova, Ljubava D.; Yakupova, Elmira I.; Vikhlyantsev, Ivan M.; Tarasova, Olga S.; Zorov, Dmitry B.; Plotnikov, Egor Y.

doi:10.3390/ijms26168100

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Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells

by

Polina A. Abramicheva

^1,*

,

Ilya A. Sokolov

^1,2,

Arina A. Druzhinina

²,

Daria M. Potashnikova

²

,

Nadezda V. Andrianova

¹

,

Dmitry S. Semenovich

^1,3

,

Vasily N. Manskikh

¹

,

Ljubava D. Zorova

^1,3,

Elmira I. Yakupova

⁴,

Ivan M. Vikhlyantsev

^5,6

,

Olga S. Tarasova

^2,7

,

Dmitry B. Zorov

^1,3

and

Egor Y. Plotnikov

^1,3,*

¹

A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia

²

School of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia

³

V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, 117997 Moscow, Russia

⁴

Institute of Biomedical Problems of the Russian Academy of Sciences, 123007 Moscow, Russia

⁵

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia

⁶

Pushchino Branch of the Federal State Budgetary Educational Institution of Higher Education “Russian Biotechnological University (BIOTECH University)”, 142290 Pushchino, Russia

⁷

Department of Physiology and Pathology, Faculty of Basic Medicine, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2025, 26(16), 8100; https://doi.org/10.3390/ijms26168100 (registering DOI)

Submission received: 29 July 2025 / Revised: 17 August 2025 / Accepted: 19 August 2025 / Published: 21 August 2025

(This article belongs to the Special Issue Role and Application of Stem Cells in Regenerative Medicine (Volume 5))

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Abstract

Renocardiac syndrome type 4 (RCS4) is a common comorbid pathology, but the mechanisms of kidney dysfunction-induced cardiac remodeling and the involvement of cardiac progenitor cells (CPCs) in this process remain unclear. The aim of this study was to investigate the structural and functional changes in the cardiac muscle in RCS4 induced by unilateral ureteral obstruction (UUO) and the role of nestin⁺ CPCs in these. Heart function and localization of nestin⁺ cells in the myocardium were assessed using nestin-GFP transgenic mice subjected to UUO for 14 and 28 days. UUO resulted in cardiac hypertrophy, accompanied by an elongation of the QRS wave on the ECG, decreased expression of Cxcl1, Cxcl9, and Il1b, reduced the number of CD11b⁺ cells, and increased in titin isoform parameters, such as T1/MHC and TT/MHC ratios, without changes in fibrosis markers. The number of nestin⁺ cells increased in the myocardium with increased duration of UUO and displayed an SCA-1⁺TBX5⁺ phenotype, consistent with CPCs. Thus, cardiac pathology in RCS4 was manifested by cardiomyocyte hypertrophy with changes in the electrophysiological phenotype of the heart, not accompanied by fibrosis or inflammation. Nestin⁺ cardiac cells retained the CPC phenotype during UUO, and their number increased, which suggests their participation in regenerative processes in the heart.

Keywords: heart; renocardiac syndrome; obstructive nephropathy; nestin-positive cells; cardiac progenitor cells; regeneration

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MDPI and ACS Style

Abramicheva, P.A.; Sokolov, I.A.; Druzhinina, A.A.; Potashnikova, D.M.; Andrianova, N.V.; Semenovich, D.S.; Manskikh, V.N.; Zorova, L.D.; Yakupova, E.I.; Vikhlyantsev, I.M.; et al. Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells. Int. J. Mol. Sci. 2025, 26, 8100. https://doi.org/10.3390/ijms26168100

AMA Style

Abramicheva PA, Sokolov IA, Druzhinina AA, Potashnikova DM, Andrianova NV, Semenovich DS, Manskikh VN, Zorova LD, Yakupova EI, Vikhlyantsev IM, et al. Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells. International Journal of Molecular Sciences. 2025; 26(16):8100. https://doi.org/10.3390/ijms26168100

Chicago/Turabian Style

Abramicheva, Polina A., Ilya A. Sokolov, Arina A. Druzhinina, Daria M. Potashnikova, Nadezda V. Andrianova, Dmitry S. Semenovich, Vasily N. Manskikh, Ljubava D. Zorova, Elmira I. Yakupova, Ivan M. Vikhlyantsev, and et al. 2025. "Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells" International Journal of Molecular Sciences 26, no. 16: 8100. https://doi.org/10.3390/ijms26168100

APA Style

Abramicheva, P. A., Sokolov, I. A., Druzhinina, A. A., Potashnikova, D. M., Andrianova, N. V., Semenovich, D. S., Manskikh, V. N., Zorova, L. D., Yakupova, E. I., Vikhlyantsev, I. M., Tarasova, O. S., Zorov, D. B., & Plotnikov, E. Y. (2025). Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells. International Journal of Molecular Sciences, 26(16), 8100. https://doi.org/10.3390/ijms26168100

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Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells

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