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Article

Functional Analysis of β-Carotene Oxygenase 2 (BCO2) Gene in Yesso Scallop (Patinopecten yessoensis)

by
Shiqi Liu
1,†,
Shuyue Wang
1,†,
Liang Zhao
1,
Tingting Li
1,
Yihan Zhang
1,
Huizhen Wang
1,2,*,
Zhenmin Bao
1,2,3 and
Xiaoli Hu
1,2,*
1
MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
2
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
3
Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(7), 3947; https://doi.org/10.3390/ijms25073947
Submission received: 22 February 2024 / Revised: 23 March 2024 / Accepted: 28 March 2024 / Published: 2 April 2024
(This article belongs to the Special Issue Aquatic Biotechnology and Its Application in Genetic Breeding)
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Abstract

Carotenoids are essential nutrients for humans and animals, and carotenoid coloration represents an important meat quality parameter for many farmed animals. Increasingly, studies have demonstrated that vertebrate carotenoid cleavage oxygenases (CCOs) are essential enzymes in carotenoid metabolism and are therefore potential candidate genes for improving carotenoid deposition. However, our understanding of carotenoid bioavailability and CCOs functions in invertebrates, particularly marine species, is currently quite limited. We previously identified that a CCO homolog, PyBCO-like 1, was the causal gene for carotenoid coloration in the ‘Haida golden scallop’, a variety of Yesso scallop (Patinopecten yessoensis) characterized by carotenoid enrichment. Here, we found that another CCO-encoding gene named PyBCO2 (β-carotene oxygenase 2) was widely expressed in P. yessoensis organs/tissues, with the highest expression in striated muscle. Inhibiting BCO2 expression in P. yessoensis through RNA interference led to increased carotenoid (pectenolone and pectenoxanthin) deposition in the striated muscle, and the color of the striated muscle changed from white to light orange. Our results indicate that PyBCO2 might be a candidate gene used for improving carotenoid content in normal Yesso scallops, and also in ‘Haida golden scallops’.
Keywords: Yesso scallop (Patinopecten yessoensis); BCO2; carotenoid; RNA interference; meat quality parameter Yesso scallop (Patinopecten yessoensis); BCO2; carotenoid; RNA interference; meat quality parameter

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MDPI and ACS Style

Liu, S.; Wang, S.; Zhao, L.; Li, T.; Zhang, Y.; Wang, H.; Bao, Z.; Hu, X. Functional Analysis of β-Carotene Oxygenase 2 (BCO2) Gene in Yesso Scallop (Patinopecten yessoensis). Int. J. Mol. Sci. 2024, 25, 3947. https://doi.org/10.3390/ijms25073947

AMA Style

Liu S, Wang S, Zhao L, Li T, Zhang Y, Wang H, Bao Z, Hu X. Functional Analysis of β-Carotene Oxygenase 2 (BCO2) Gene in Yesso Scallop (Patinopecten yessoensis). International Journal of Molecular Sciences. 2024; 25(7):3947. https://doi.org/10.3390/ijms25073947

Chicago/Turabian Style

Liu, Shiqi, Shuyue Wang, Liang Zhao, Tingting Li, Yihan Zhang, Huizhen Wang, Zhenmin Bao, and Xiaoli Hu. 2024. "Functional Analysis of β-Carotene Oxygenase 2 (BCO2) Gene in Yesso Scallop (Patinopecten yessoensis)" International Journal of Molecular Sciences 25, no. 7: 3947. https://doi.org/10.3390/ijms25073947

APA Style

Liu, S., Wang, S., Zhao, L., Li, T., Zhang, Y., Wang, H., Bao, Z., & Hu, X. (2024). Functional Analysis of β-Carotene Oxygenase 2 (BCO2) Gene in Yesso Scallop (Patinopecten yessoensis). International Journal of Molecular Sciences, 25(7), 3947. https://doi.org/10.3390/ijms25073947

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