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Article

Microcurrent-Mediated Modulation of Myofibroblasts for Cardiac Repair and Regeneration

by
Dipthi Bachamanda Somesh
1,*,
Karsten Jürchott
2,
Thomas Giesel
1,
Thomas Töllner
1,
Alexander Prehn
1,
Jan-Peter Richters
1,
Dragana Kosevic
3,
Jesus Eduardo Rame
4,
Peter Göttel
1 and
Johannes Müller
1
1
Berlin Heals, 59-61 Knesebeck Str, 10719 Berlin, Germany
2
BIH Center for Regenerative Therapies, Regenerative Immunology and Aging, BIH Immunomics, Berlin Institute of Health at Charité, Universitätsmedizin Berlin, 13353 Berlin, Germany
3
Dedinje Cardiovascular Institute, Dedinje University Hospital, 11040 Belgrade, Serbia
4
Jefferson Heart Institute, Thomas Jefferson University Hospital, Philadelphia, PA 19107, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(6), 3268; https://doi.org/10.3390/ijms25063268
Submission received: 1 February 2024 / Revised: 5 March 2024 / Accepted: 9 March 2024 / Published: 13 March 2024
(This article belongs to the Special Issue Research Progress on the Mechanism and Treatment of Cardiomyopathy)
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Abstract

Cardiovascular diseases are a significant cause of illness and death worldwide, often resulting in myofibroblast differentiation, pathological remodeling, and fibrosis, characterized by excessive extracellular matrix protein deposition. Treatment options for cardiac fibrosis that can effectively target myofibroblast activation and ECM deposition are limited, necessitating an unmet need for new therapeutic approaches. In recent years, microcurrent therapy has demonstrated promising therapeutic effects, showcasing its translational potential in cardiac care. This study therefore sought to investigate the effects of microcurrent therapy on cardiac myofibroblasts, aiming to unravel its potential as a treatment for cardiac fibrosis and heart failure. The experimental design involved the differentiation of primary rat cardiac fibroblasts into myofibroblasts. Subsequently, these cells were subjected to microcurrent (MC) treatment at 1 and 2 µA/cm2 DC with and without polarity reversal. We then investigated the impact of microcurrent treatment on myofibroblast cell behavior, including protein and gene expression, by performing various assays and analyses comparing them to untreated myofibroblasts and cardiac fibroblasts. The application of microcurrents resulted in distinct transcriptional signatures and improved cellular processes. Gene expression analysis showed alterations in myofibroblast markers, extracellular matrix components, and pro-inflammatory cytokines. These observations show signs of microcurrent-mediated reversal of myofibroblast phenotype, possibly reducing cardiac fibrosis, and providing insights for cardiac tissue repair.
Keywords: cardiovascular diseases; cardiac fibroblasts; fibrosis; myofibroblasts; microcurrent cardiovascular diseases; cardiac fibroblasts; fibrosis; myofibroblasts; microcurrent

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MDPI and ACS Style

Bachamanda Somesh, D.; Jürchott, K.; Giesel, T.; Töllner, T.; Prehn, A.; Richters, J.-P.; Kosevic, D.; Eduardo Rame, J.; Göttel, P.; Müller, J. Microcurrent-Mediated Modulation of Myofibroblasts for Cardiac Repair and Regeneration. Int. J. Mol. Sci. 2024, 25, 3268. https://doi.org/10.3390/ijms25063268

AMA Style

Bachamanda Somesh D, Jürchott K, Giesel T, Töllner T, Prehn A, Richters J-P, Kosevic D, Eduardo Rame J, Göttel P, Müller J. Microcurrent-Mediated Modulation of Myofibroblasts for Cardiac Repair and Regeneration. International Journal of Molecular Sciences. 2024; 25(6):3268. https://doi.org/10.3390/ijms25063268

Chicago/Turabian Style

Bachamanda Somesh, Dipthi, Karsten Jürchott, Thomas Giesel, Thomas Töllner, Alexander Prehn, Jan-Peter Richters, Dragana Kosevic, Jesus Eduardo Rame, Peter Göttel, and Johannes Müller. 2024. "Microcurrent-Mediated Modulation of Myofibroblasts for Cardiac Repair and Regeneration" International Journal of Molecular Sciences 25, no. 6: 3268. https://doi.org/10.3390/ijms25063268

APA Style

Bachamanda Somesh, D., Jürchott, K., Giesel, T., Töllner, T., Prehn, A., Richters, J.-P., Kosevic, D., Eduardo Rame, J., Göttel, P., & Müller, J. (2024). Microcurrent-Mediated Modulation of Myofibroblasts for Cardiac Repair and Regeneration. International Journal of Molecular Sciences, 25(6), 3268. https://doi.org/10.3390/ijms25063268

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