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Volume 25
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10.3390/ijms25147591
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Article

A Single Injection of ADRCs Does Not Prevent AAA Formation in Rats in a Randomized Blinded Design

by
Egle Kavaliunaite
1,2,
Pratibha Dhumale
3,4,
Charlotte Harken Jensen
3,4,
Søren P. Sheikh
5,
Jes S. Lindholt
2 and
Jane Stubbe
1,*
1
Cardiovascular and Renal Research Unit, Institute for Molecular Medicine, University of Southern Denmark, 5230 Odense M, Denmark
2
Department of Cardiac, Thoracic and Vascular Surgery, Odense University Hospital (OUH), 5000 Odense C, Denmark
3
Department of Clinical Research, University of Southern Denmark (SDU), 5230 Odense M, Denmark
4
Department of Clinical Biochemistry, Odense University Hospital (OUH), 5000 Odense C, Denmark
5
Open Patient Data Explorative Network, Institute of Clinical Research, Odense University Hospital (OUH), 5000 Odense C, Denmark
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(14), 7591; https://doi.org/10.3390/ijms25147591
Submission received: 14 May 2024 / Revised: 28 June 2024 / Accepted: 1 July 2024 / Published: 10 July 2024
(This article belongs to the Special Issue Adipose Tissue and Adipose Tissue-Derived Stem Cells in Regenerative Medicine)
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Abstract

There is a pressing need for alternative medical treatments for abdominal aortic aneurysms (AAAs). Mesenchymal regenerative cells derived from adipose tissue (ADRCs) have shown potential in modulating the inflammation and immune responses that drive AAA progression. We hypothesized that ADRCs could reduce inflammation and preserve vascular integrity, potentially slowing the progression of AAA. In our study, subcutaneous adipose tissue was harvested from male Sprague Dawley rats, from which ADRCs were isolated. AAA was induced in these rats using intraluminal porcine pancreatic elastase, followed by intravenous administration of either ADRCs (106 cells) or saline (0.1 mL). We monitored the progression of AAA through weekly ultrasound, and the rats were sacrificed on day 28 for histological analysis. Our results showed no significant difference in the inner abdominal aortic diameter at day 28 between the control group (172% ± 73%, n = 17) and the ADRC-treated group (181% ± 75%, n = 15). Histological analyses of AAA cross-sections also revealed no significant difference in the infiltration of neutrophils or macrophages between the two groups. Furthermore, the integrity and content of elastin in the tunica media were similar between groups. These findings indicate that a single injection of ADRCs does not inhibit the development of AAA in rats in a randomized blinded study.
Keywords: stem cells; abdominal aortic aneurysm; regenerative medicine stem cells; abdominal aortic aneurysm; regenerative medicine

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MDPI and ACS Style

Kavaliunaite, E.; Dhumale, P.; Jensen, C.H.; Sheikh, S.P.; Lindholt, J.S.; Stubbe, J. A Single Injection of ADRCs Does Not Prevent AAA Formation in Rats in a Randomized Blinded Design. Int. J. Mol. Sci. 2024, 25, 7591. https://doi.org/10.3390/ijms25147591

AMA Style

Kavaliunaite E, Dhumale P, Jensen CH, Sheikh SP, Lindholt JS, Stubbe J. A Single Injection of ADRCs Does Not Prevent AAA Formation in Rats in a Randomized Blinded Design. International Journal of Molecular Sciences. 2024; 25(14):7591. https://doi.org/10.3390/ijms25147591

Chicago/Turabian Style

Kavaliunaite, Egle, Pratibha Dhumale, Charlotte Harken Jensen, Søren P. Sheikh, Jes S. Lindholt, and Jane Stubbe. 2024. "A Single Injection of ADRCs Does Not Prevent AAA Formation in Rats in a Randomized Blinded Design" International Journal of Molecular Sciences 25, no. 14: 7591. https://doi.org/10.3390/ijms25147591

APA Style

Kavaliunaite, E., Dhumale, P., Jensen, C. H., Sheikh, S. P., Lindholt, J. S., & Stubbe, J. (2024). A Single Injection of ADRCs Does Not Prevent AAA Formation in Rats in a Randomized Blinded Design. International Journal of Molecular Sciences, 25(14), 7591. https://doi.org/10.3390/ijms25147591

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