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Article

Identification and Functional Analysis of KH Family Genes Associated with Salt Stress in Rice

by
Qinyu Xie
1,
Yutong Zhang
1,
Mingming Wu
2,
Youheng Chen
1,
Yingwei Wang
1,
Qinzong Zeng
1,
Yuliang Han
1,
Siqi Zhang
1,
Juncheng Zhang
1,
Tao Chen
1 and
Maohong Cai
1,*
1
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China
2
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(11), 5950; https://doi.org/10.3390/ijms25115950
Submission received: 17 March 2024 / Revised: 22 May 2024 / Accepted: 23 May 2024 / Published: 29 May 2024
(This article belongs to the Special Issue Advance in Plant Abiotic Stress)
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Abstract

Salinity stress has a great impact on crop growth and productivity and is one of the major factors responsible for crop yield losses. The K-homologous (KH) family proteins play vital roles in regulating plant development and responding to abiotic stress in plants. However, the systematic characterization of the KH family in rice is still lacking. In this study, we performed genome-wide identification and functional analysis of KH family genes and identified a total of 31 KH genes in rice. According to the homologs of KH genes in Arabidopsis thaliana, we constructed a phylogenetic tree with 61 KH genes containing 31 KH genes in Oryza sativa and 30 KH genes in Arabidopsis thaliana and separated them into three major groups. In silico tissue expression analysis showed that the OsKH genes are constitutively expressed. The qRT-PCR results revealed that eight OsKH genes responded strongly to salt stresses, and OsKH12 exhibited the strongest decrease in expression level, which was selected for further study. We generated the Oskh12-knockout mutant via the CRISPR/Cas9 genome-editing method. Further stress treatment and biochemical assays confirmed that Oskh12 mutant was more salt-sensitive than Nip and the expression of several key salt-tolerant genes in Oskh12 was significantly reduced. Taken together, our results shed light on the understanding of the KH family and provide a theoretical basis for future abiotic stress studies in rice.
Keywords: KH family; salt stress; rice; genome-wide identification KH family; salt stress; rice; genome-wide identification

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MDPI and ACS Style

Xie, Q.; Zhang, Y.; Wu, M.; Chen, Y.; Wang, Y.; Zeng, Q.; Han, Y.; Zhang, S.; Zhang, J.; Chen, T.; et al. Identification and Functional Analysis of KH Family Genes Associated with Salt Stress in Rice. Int. J. Mol. Sci. 2024, 25, 5950. https://doi.org/10.3390/ijms25115950

AMA Style

Xie Q, Zhang Y, Wu M, Chen Y, Wang Y, Zeng Q, Han Y, Zhang S, Zhang J, Chen T, et al. Identification and Functional Analysis of KH Family Genes Associated with Salt Stress in Rice. International Journal of Molecular Sciences. 2024; 25(11):5950. https://doi.org/10.3390/ijms25115950

Chicago/Turabian Style

Xie, Qinyu, Yutong Zhang, Mingming Wu, Youheng Chen, Yingwei Wang, Qinzong Zeng, Yuliang Han, Siqi Zhang, Juncheng Zhang, Tao Chen, and et al. 2024. "Identification and Functional Analysis of KH Family Genes Associated with Salt Stress in Rice" International Journal of Molecular Sciences 25, no. 11: 5950. https://doi.org/10.3390/ijms25115950

APA Style

Xie, Q., Zhang, Y., Wu, M., Chen, Y., Wang, Y., Zeng, Q., Han, Y., Zhang, S., Zhang, J., Chen, T., & Cai, M. (2024). Identification and Functional Analysis of KH Family Genes Associated with Salt Stress in Rice. International Journal of Molecular Sciences, 25(11), 5950. https://doi.org/10.3390/ijms25115950

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