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Volume 25
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Article

Adar Regulates Drosophila melanogaster Spermatogenesis via Modulation of BMP Signaling

by
Qian Zhang
1,2,
Xinxin Fan
2,
Fang Fu
2,
Yuedan Zhu
2,
Guanzheng Luo
1 and
Haiyang Chen
2,*
1
MOE Key Laboratory of Gene Function and Regulation, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
2
Laboratory of Stem Cell and Aging Research, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Respiratory Health and Multimorbidity and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(11), 5643; https://doi.org/10.3390/ijms25115643
Submission received: 11 April 2024 / Revised: 16 May 2024 / Accepted: 20 May 2024 / Published: 22 May 2024
(This article belongs to the Section Molecular Biology)
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Abstract

The dynamic process of Drosophila spermatogenesis involves asymmetric division, mitosis, and meiosis, which ultimately results in the production of mature spermatozoa. Disorders of spermatogenesis can lead to infertility in males. ADAR (adenosine deaminase acting on RNA) mutations in Drosophila cause male infertility, yet the causative factors remain unclear. In this study, immunofluorescence staining was employed to visualize endogenous ADAR proteins and assess protein levels via fluorescence-intensity analysis. In addition, the early differentiation disorders and homeostatic alterations during early spermatogenesis in the testes were examined through quantification of transit-amplifying region length, counting the number of GSCs (germline stem cells), and fertility experiments. Our findings suggest that deletion of ADAR causes testicular tip transit-amplifying cells to accumulate and become infertile in older male Drosophila. By overexpressing ADAR in early germline cells, male infertility can be partially rescued. Transcriptome analysis showed that ADAR maintained early spermatogenesis homeostasis through the bone-morphogenetic-protein (BMP) signaling pathway. Taken together, these findings have the potential to help explore the role of ADAR in early spermatogenesis.
Keywords: Drosophila; early spermatogenesis; male infertility; ADAR; BMP signaling Drosophila; early spermatogenesis; male infertility; ADAR; BMP signaling

Share and Cite

MDPI and ACS Style

Zhang, Q.; Fan, X.; Fu, F.; Zhu, Y.; Luo, G.; Chen, H. Adar Regulates Drosophila melanogaster Spermatogenesis via Modulation of BMP Signaling. Int. J. Mol. Sci. 2024, 25, 5643. https://doi.org/10.3390/ijms25115643

AMA Style

Zhang Q, Fan X, Fu F, Zhu Y, Luo G, Chen H. Adar Regulates Drosophila melanogaster Spermatogenesis via Modulation of BMP Signaling. International Journal of Molecular Sciences. 2024; 25(11):5643. https://doi.org/10.3390/ijms25115643

Chicago/Turabian Style

Zhang, Qian, Xinxin Fan, Fang Fu, Yuedan Zhu, Guanzheng Luo, and Haiyang Chen. 2024. "Adar Regulates Drosophila melanogaster Spermatogenesis via Modulation of BMP Signaling" International Journal of Molecular Sciences 25, no. 11: 5643. https://doi.org/10.3390/ijms25115643

APA Style

Zhang, Q., Fan, X., Fu, F., Zhu, Y., Luo, G., & Chen, H. (2024). Adar Regulates Drosophila melanogaster Spermatogenesis via Modulation of BMP Signaling. International Journal of Molecular Sciences, 25(11), 5643. https://doi.org/10.3390/ijms25115643

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