Next Article in Journal
A Method for Increasing the Robustness of Stable Feature Selection for Biomarker Discovery in Molecular Medicine Developed Using Serum Small Extracellular Vesicle Associated miRNAs and the Barrett’s Oesophagus Disease Spectrum
Next Article in Special Issue
Genomic Characterization of Uropathogenic Escherichia coli Isolates from Tertiary Hospitals in Riyadh, Saudi Arabia
Previous Article in Journal
In Vitro and In Vivo Effects of the Combination of Polypurine Reverse Hoogsteen Hairpins against HER-2 and Trastuzumab in Breast Cancer Cells
Previous Article in Special Issue
Collateral Sensitivity to Fosfomycin of Tobramycin-Resistant Mutants of Pseudomonas aeruginosa Is Contingent on Bacterial Genomic Background
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 24
Issue 8
10.3390/ijms24087077
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Novel Hybrid Platform for Live/Dead Bacteria Accurate Sorting by On-Chip DEP Device

by
Annarita di Toma
1,
Giuseppe Brunetti
1,
Maria Serena Chiriacò
2,
Francesco Ferrara
2 and
Caterina Ciminelli
1,*
1
Optoelectronics Laboratory, Politecnico di Bari, Via E. Orabona 6, 70125 Bari, Italy
2
CNR NANOTEC—Institute of Nanotechnology, Via per Monteroni, 73100 Lecce, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(8), 7077; https://doi.org/10.3390/ijms24087077
Submission received: 10 March 2023 / Revised: 23 March 2023 / Accepted: 5 April 2023 / Published: 11 April 2023
(This article belongs to the Special Issue Antimicrobial Resistance—New Insights 2.0)
Browse Figures
Review Reports Versions Notes

Abstract

According to the World Health Organization (WHO) forecasts, Antimicrobial Resistance (AMR) will be the leading cause of death worldwide in the next decades. To prevent this phenomenon, rapid Antimicrobial Susceptibility Testing (AST) techniques are required to drive the selection of the most suitable antibiotic and its dosage. In this context, we propose an on-chip platform, based on a micromixer and a microfluidic channel, combined with a pattern of engineered electrodes to exploit the di-electrophoresis (DEP) effect. The role of the micromixer is to ensure the proper interaction of the antibiotic with the bacteria over a long time (≈1 h), and the DEP-based microfluidic channel enables the efficient sorting of live from dead bacteria. A sorting efficiency of more than 98%, with low power consumption (Vpp = 1 V) and time response of 5 s, within a chip footprint of ≈86 mm2, has been calculated, which makes the proposed system very attractive and innovative for efficient and rapid monitoring of the antimicrobial susceptibility at the single-bacterium level in next-generation medicine.
Keywords: di-electrophoresis; Escherichia coli; microfluidics; antimicrobial susceptibility testing; chip-scale platform di-electrophoresis; Escherichia coli; microfluidics; antimicrobial susceptibility testing; chip-scale platform

Share and Cite

MDPI and ACS Style

di Toma, A.; Brunetti, G.; Chiriacò, M.S.; Ferrara, F.; Ciminelli, C. A Novel Hybrid Platform for Live/Dead Bacteria Accurate Sorting by On-Chip DEP Device. Int. J. Mol. Sci. 2023, 24, 7077. https://doi.org/10.3390/ijms24087077

AMA Style

di Toma A, Brunetti G, Chiriacò MS, Ferrara F, Ciminelli C. A Novel Hybrid Platform for Live/Dead Bacteria Accurate Sorting by On-Chip DEP Device. International Journal of Molecular Sciences. 2023; 24(8):7077. https://doi.org/10.3390/ijms24087077

Chicago/Turabian Style

di Toma, Annarita, Giuseppe Brunetti, Maria Serena Chiriacò, Francesco Ferrara, and Caterina Ciminelli. 2023. "A Novel Hybrid Platform for Live/Dead Bacteria Accurate Sorting by On-Chip DEP Device" International Journal of Molecular Sciences 24, no. 8: 7077. https://doi.org/10.3390/ijms24087077

APA Style

di Toma, A., Brunetti, G., Chiriacò, M. S., Ferrara, F., & Ciminelli, C. (2023). A Novel Hybrid Platform for Live/Dead Bacteria Accurate Sorting by On-Chip DEP Device. International Journal of Molecular Sciences, 24(8), 7077. https://doi.org/10.3390/ijms24087077

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop