MC-PRPA-HLFIA Cascade Detection System for Point-of-Care Testing Pan-Drug-Resistant Genes in Urinary Tract Infection Samples
, Wenchong Shan, Leina Dou, Weishuai Zhai, Yang Wang, Jianzhong Shen * and Kai Wen *
Round 1
Reviewer 1 Report
Dear all,
I have read the manuscript thoroughly. I donot have major comments and I accept to publish the current fromof the manuscript.
Author Response
Thank you for your approval of this study and good luck with your career!
Reviewer 2 Report
Type of manuscript: Article
Title: MC-PRPA-HLFIA cascade detection system for point-of-care testing
pan-drug resistant genes in urinary tract infection samples
Journal: International Journal of Molecular Sciences
Review report: The authors present the design and application of a system for the determination of antibiotic resistance genes, NDM, KPC, mcr-1 and tetX, that confer resistance to carbapenems, colistine and tigeclycline. This resistance genes are reported as important in the determination of pandrogo-resistant bacteria isolated from urinary tract infection (ITU). It is a point-of-care test based on a combination of rapid lysis, polimerase recombinase gene amplification, and a lateral flow immunoassay.
The manuscript is clear, well structured, properly referenced, easy to read and the topic is relevant.
The authors presented a properly squeme to show the principle of the detection system. The results are presented in appropiated tables and figures and are supported by statistical test and supplementary material.
Specific comments: After reviewing the manuscript I suggest the following minor corrections:
Page 2 Line add the meaning of HRP and AEC
Page 5 line 139 include references related to previous studies
Line 146-150 rewrite the paragraph by adding the DNA concentration and volumen data used in the standardization for all genes tested
Page 9 Line 234-236 rewrite the paragraph
Line 241 change the frase because 102 UFC/mL is not higher than 360 UFC/mL
Page 10 line 282 change the word “bacteria” to the word “strains” and include space after ….. respectively.
Line 282-285 explain the reason of this paragraph
line 306 change the word “devised” to “designed”
line 318 add the incubation temperatura of the mixture
Line 320 change the word “spiked” to “added”
Page 11 line 324 remove the space in the word “absorb bent”
line 327 the frase is not understood replace by the phrase “ 1ul of each antibody”
line 329 change the phrase to “ individual strips LFIA of 3.0 mm thick were obtained
line 333 complete the sentence like this: “DNA extraction was carried out using….”
line 360 change “events” por “pan-drug resistance genes…”
Author Response
Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 3 Report
The chosen topic is in line with the real trend in UTIs.
As a note, the "Discussion" section is a little bit too short. It could be made longer by comparing it to other recent studies.
This study can also have a paragraph about strengths and limitations.
Author Response
Please see the attachment.
Author Response File: Author Response.docx
